Membrane perturbation of fullerene and graphene oxide distinguished by pore-forming peptide melittin

Zhang, Che; Ge, Yuke; Chen, Zhonglan; Liu, Jiaojiao; Zhang, Mengling; Yang, Kai; Yuan, Bing

doi:10.1016/j.carbon.2021.04.081

Cited by 12 publications

(12 citation statements)

References 61 publications

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“…In addition, the inherent hydrophobic structure of fullerenes can cause mechanical disruption of the cell membrane. Even at low concentrations, fullerenes can adhere to and insert into the cell membrane, leading to the change in membrane permeability and leakage of intracellular substances, eventually causing bacterial death ( Zhang et al, 2021 ). Furthermore, fullerenes can cause protein denaturation under irradiation ( Radic et al, 2014 ).…”

Section: Antibacterial Mechanisms Of Fullerene-based Antimicrobial Ph...mentioning

confidence: 99%

Application of Fullerenes as Photosensitizers for Antimicrobial Photodynamic Inactivation: A Review

Hou

Shi

et al. 2022

Front. Microbiol.

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Antimicrobial photodynamic inactivation (aPDI) is a newly emerged treatment approach that can effectively address the issue of multidrug resistance resulting from the overuse of antibiotics. Fullerenes can be used as promising photosensitizers (PSs) for aPDI due to the advantages of high triplet state yields, good photostability, wide antibacterial spectrum, and permissibility of versatile functionalization. This review introduces the photodynamic activities of fullerenes and the up-to-date understanding of the antibacterial mechanisms of fullerene-based aPDI. The most recent works on the functionalization of fullerenes and the application of fullerene derivatives as PSs for aPDI are also summarized. Finally, certain remaining challenges are emphasized to provide guidance on future research directions for achieving clinical application of fullerene-based aPDI.

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Section: Antibacterial Mechanisms Of Fullerene-based Antimicrobial Ph...mentioning

confidence: 99%

Application of Fullerenes as Photosensitizers for Antimicrobial Photodynamic Inactivation: A Review

Hou

Shi

et al. 2022

Front. Microbiol.

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“…The minimal inhibitory concentration (MIC) of the agent was determined using the broth microdilution method [ 16 , 22 ]. A volume of 100 µL broth, containing 10 5 CFU mL −1 of E. coli , was added to each well of the 96-well plates.…”

Section: Methodsmentioning

confidence: 99%

“…Bacteria were treated with GO, PMB, and PMB@GO (at 5× MIC concentrations) and fixed with 2.5% glutaraldehyde overnight at 4 °C. After washing with PBS buffer, the bacterial cells were dehydrated through sequential treatments of 20%, 40%, 60%, 80%, and 100% ethanol for 30 min [ 16 , 22 ], dropped onto a cleaned silicon wafer to lyophilize, and imaged using a scanning electron microscope (SEM; SU8010, HITACHI, Tokyo, Japan) after gold coating.…”

Section: Methodsmentioning

confidence: 99%

“…Nanoparticles (NPs) with engineered structure provide a promising route to aid the membrane interaction of many antibacterial agents [ 15 , 16 , 17 , 18 , 19 , 20 ]. Golden, silver, and polymetric NPs are surficial-decorated with antibiotics or antimicrobial peptides, which show strengthened membrane-disrupting and bacterial-killing ability [ 18 , 19 , 21 , 22 , 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

“…This enhancement is generally ascribed to the increased local density of positive charges and antimicrobial molecules [ 17 , 18 , 19 , 22 ]. Among NPs, graphene oxide nanosheets (GO) demonstrate great potential in biomedical applications due to advantages including good water dispersion, low biological toxicity, easy modification, and mass production [ 15 , 16 , 17 , 25 , 26 ]. Specifically, the unique two-dimensional structure and amphiphilic surface endow GO to penetrate the cell membranes like a “nano-knife” and even extract lipids from the outer leaflet, inducing physical disturbance to the membrane structure [ 15 , 17 , 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

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Synergistic Membrane Disturbance Improves the Antibacterial Performance of Polymyxin B

Zhang

Sun

et al. 2022

Polymers

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Drug-resistant Gram-negative bacteria pose a serious threat to public health, and polymyxin B (PMB) is clinically used as a last-line therapy for the treatment of infections caused by these pathogens. However, the appearance of PMB resistance calls for an effort to develop new approaches to improve its antibacterial performance. In this work, a new type of nanocomposite, composed of PMB molecules being chemically decorated on the surface of graphene oxide (GO) nanosheets, was designed, which showed potent antibacterial ability through synergistically and physically disturbing the bacterial membrane. The as-fabricated PMB@GO nanocomposites demonstrated an enhanced bacterial-killing efficiency, with a minimum inhibitory concentration (MIC) value half of that of free PMB (with an MIC value as low as 0.5 μg mL−1 over Escherichia coli), and a bacterial viability less than one fourth of that of PMB (with a bacterial reduction of 60% after 3 h treatment, and 90% after 6 h incubation). Furthermore, the nanocomposite displayed moderate cytotoxicity or hemolysis effect, with cellular viabilities over 85% at concentrations up to 16 times the MIC value. Studies on antibacterial mechanism revealed that the synergy between PMB molecules and GO nanosheets greatly facilitated the vertical insertion of the nanocomposite into the lipid membrane, leading to membrane disturbance and permeabilization. Our results demonstrate a physical mechanism for improving the antibacterial performance of PMB and developing advanced antibacterial agents for better clinic uses.

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Transition between Different Diffusion Modes of Individual Lipids during the Membrane‐Specific Action of As‐CATH4 Peptides

Cheng

et al. 2023

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The cell membrane permeabilization ability of immune defense antimicrobial peptides (AMPs) is widely applied in biomedicine. Although the mechanisms of peptide–membrane interactions have been widely investigated, analyses at the molecular level are still lacking. Herein, the membrane‐specific action of a native AMP, As‐CATH4, is investigated using a single‐lipid tracking method in combination with live cell and model membrane assays conducted at different scales. The peptide–membrane interaction process is characterized by analyzing single‐lipid diffusion behaviors. As‐CATH4 exhibits potent antimicrobial activity through bacterial membrane permeabilization, with moderate cytotoxicity against mammalian cells. In‐plane diffusion analyses of individual lipids show that the lipid molecules exhibit non‐Gaussian and heterogeneous diffusion behaviors in both pristine and peptide‐treated membranes, which can be decomposed into two Gaussian subgroups corresponding to normal‐ and slow‐diffusive lipids. Assessment of the temporal evolution of these two diffusion modes of lipids reveal that the peptide action states of As‐CATH4 include surface binding, transmembrane defect formation, and dynamic equilibrium. The action mechanisms of As‐CATH4 at varying concentrations and against different membranes are distinguished. This work resolves the simultaneous mixed diffusion mechanisms of single lipids in biomimetic cell membranes, especially during dynamic membrane permeabilization by AMPs.

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Membrane perturbation of fullerene and graphene oxide distinguished by pore-forming peptide melittin

Cited by 12 publications

References 61 publications

Application of Fullerenes as Photosensitizers for Antimicrobial Photodynamic Inactivation: A Review

Application of Fullerenes as Photosensitizers for Antimicrobial Photodynamic Inactivation: A Review

Synergistic Membrane Disturbance Improves the Antibacterial Performance of Polymyxin B

Transition between Different Diffusion Modes of Individual Lipids during the Membrane‐Specific Action of As‐CATH4 Peptides

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