Membrane Mimetic Chemistry in Artificial Cells

Vance, Jacob A; Devaraj, Neal K.

doi:10.1021/jacs.1c03436

Cited by 63 publications

(46 citation statements)

References 77 publications

(168 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, there is considerable interest in exploring new artificial lipid architectures capable of recreating the physiological environment of natural organelles. The complex topology observed in lipid mesophases could allow them to serve as a generic model system of the convoluted membrane architecture in membrane‐bound organelles that would help to elucidate the functional roles of non‐lamellar structures in biology [14] . Lipid mesophases consist of a highly curved continuous bilayer that encompasses an interconnected aqueous channel network [15,16] .…”

Section: Introductionmentioning

confidence: 99%

“…The complex topology observed in lipid mesophases could allow them to serve as a generic model system of the convoluted membrane architecture in membrane-bound organelles that would help to elucidate the functional roles of non-lamellar structures in biology. [14] Lipid mesophases consist of a highly curved continuous bilayer that encompasses an interconnected aqueous channel network. [15,16] Given the utility of such bi-continuous architectures for protein crystallization, [17][18][19] drug delivery, [20][21][22] and as food emulsifiers, [23] the applications of lipid mesophases in mimicking organelle function is expanding.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Controlling Protein Enrichment in Lipid Sponge Phase Droplets using SNAP‐Tag Bioconjugation

et al. 2022

Self Cite

View full text Add to dashboard Cite

All cells use organized lipid compartments to facilitate specific biological functions. Membrane‐bound organelles create defined spatial environments that favor unique chemical reactions while isolating incompatible biological processes. Despite the fundamental role of cellular organelles, there is a scarcity of methods for preparing functional artificial lipid‐based compartments. Here, we demonstrate a robust bioconjugation system for sequestering proteins into zwitterionic lipid sponge phase droplets. Incorporation of benzylguanine (BG)‐modified phospholipids that form stable covalent linkages with an O6‐methylguanine DNA methyltransferase (SNAP‐tag) fusion protein enables programmable control of protein capture. We show that this methodology can be used to anchor hydrophilic proteins at the lipid‐aqueous interface, concentrating them within an accessible but protected chemical environment. SNAP‐tag technology enables the integration of proteins that regulate complex biological functions in lipid sponge phase droplets, and should facilitate the development of advanced lipid‐based artificial organelles.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Controlling Protein Enrichment in Lipid Sponge Phase Droplets using SNAP‐Tag Bioconjugation

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…This leads to the completion of cytokinesis [5]. Membrane growth comes up as an essential problem to take note of in the study of cell division models [48,49].…”

Section: Future Directionsmentioning

confidence: 99%

Thermo-Statistical Effects of Inclusions on Vesicles: Division into Multispheres and Polyhedral Deformation

Natsume

2022

Membranes

View full text Add to dashboard Cite

The construction of simple cellular models has attracted much attention as a way to explore the origin of life or elucidate the mechanisms of cell division. In the absence of complex regulatory systems, some bacteria spontaneously divide through thermostatistically elucidated mechanisms, and incorporating these simple physical principles could help to construct primitive or artificial cells. Because thermodynamic interactions play an essential role in such mechanisms, this review discusses the thermodynamic aspects of spontaneous division models of vesicles that contain a high density of inclusions, with their membrane serving as a boundary. Vesicles with highly dense inclusions are deformed according to the volume-to-area ratio. The phase separation of beads at specific intermediate volume fractions and the associated polyhedral deformation of the membrane are considered in relation to the Alder transition. Current advances in the development of a membrane-growth vesicular model are summarized. The thermostatistical understanding of these mechanisms could become a cornerstone for the construction of vesicular models that display spontaneous cell division.

show abstract

“…A homogeneous population can, nevertheless, be selected through post-synthetic procedures, such as extrusion or chromatographic separation [ 30 , 31 , 32 , 33 , 34 ]. A proper design of the composition of a GV membrane is important for studying the processes of shape deformation, which eventually leads to division [ 35 , 36 ], and this aspect contributed to boosting the research of more sophisticated stimuli-responsive membranes, which is having a great impact on technological applications, especially those based on lipid chemistry [ 37 , 38 , 39 ]. In addition to GVs, unilamellar small vesicles (SUVs, d ∼ 20–100 nm), large (LUVs, d ∼ 100 nm–1

m) and multilamellar large vesicles (MLVs) are widely employed as models for studying the shape deformations of cells.…”

Section: Introductionmentioning

confidence: 99%

Shape Deformation, Budding and Division of Giant Vesicles and Artificial Cells: A Review

Miele

Holló

Lagzi

et al. 2022

Life

View full text Add to dashboard Cite

The understanding of the shape-change dynamics leading to the budding and division of artificial cells has gained much attention in the past few decades due to an increased interest in designing stimuli-responsive synthetic systems and minimal models of biological self-reproduction. In this respect, membranes and their composition play a fundamental role in many aspects related to the stability of the vesicles: permeability, elasticity, rigidity, tunability and response to external changes. In this review, we summarise recent experimental and theoretical work dealing with shape deformation and division of (giant) vesicles made of phospholipids and/or fatty acids membranes. Following a classic approach, we divide the strategies used to destabilise the membranes into two different types, physical (osmotic stress, temperature and light) and chemical (addition of amphiphiles, the addition of reactive molecules and pH changes) even though they often act in synergy when leading to a complete division process. Finally, we review the most important theoretical methods employed to describe the equilibrium shapes of giant vesicles and how they provide ways to explain and control the morphological changes leading from one equilibrium structure to another.

show abstract

Membrane Mimetic Chemistry in Artificial Cells

Cited by 63 publications

References 77 publications

Controlling Protein Enrichment in Lipid Sponge Phase Droplets using SNAP‐Tag Bioconjugation

Controlling Protein Enrichment in Lipid Sponge Phase Droplets using SNAP‐Tag Bioconjugation

Thermo-Statistical Effects of Inclusions on Vesicles: Division into Multispheres and Polyhedral Deformation

Shape Deformation, Budding and Division of Giant Vesicles and Artificial Cells: A Review

Contact Info

Product

Resources

About