Membrane Localization of β-Catenin in Prostate Cancer PC3 Cells Treated with <i>Teucrium persicum</i> Boiss. Extract

Barati, Narges; Tafrihi, Majid; Najafi, Seyed Mahmoud Arab

doi:10.1080/01635581.2021.1961829

Cited by 2 publications

(1 citation statement)

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“…MiR-552 is a newly discovered miRNA; its function and mechanism of action in biological processes and diseases are not entirely understood 24 . Previous studies showed that miR-552 promotes colorectal cancer cell proliferation and migration by directly targeting DACH1 via the Wnt/β-catenin signaling pathway 18 , 25 . The up-regulation of miR-552 was reported in ovarian cancer, especially in metastasis and recurrence 24 .…”

Section: Introductionmentioning

confidence: 99%

Study of DACH1 Expression and its Epigenetic Regulators as Possible Breast Cancer-Related Biomarkers

Nasirpour¹,

Salimi²,

Majidi³

et al. 2023

AJMB

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Background: Breast carcinogenesis involves both genetic and epigenetic changes. DNA methylation, as well as micro-RNA regulations, are the significant epigenetic phenomena dysregulated in breast cancer. Herein, the expression of DACH1 as a tumor suppressor gene and its promoter methylation status was analyzed in breast cancer tumors. Also, the expression of three micro RNAs (miR-217, miR-6807-3p, and miR-552), which had been previously reported to target DACH1, was assessed. Methods: The SYBR green-based Real-Time reverse transcription-PCR was used to determine DACH1 and micro-RNAs (miR-217, miR-6807-3p, and miR-552) expression in 120 ductal breast cancer tumors compared with standard control. Also, the promoter methylation pattern of DACH1 was investigated using the Methylation-specific PCR technique. Results: DACH1 expression was significantly down-regulated in breast tumors (p< 0.05). About 33.5% of tumors showed DACH1 promoter hyper-methylation. The studied micro-RNAs, expression was negatively correlated with DACH1 expression. The highest expressions of miRNAs and higher DACH1 promoter methylation were observed in advanced cancer situations. The Kaplan-Meier survival curves indicated that the overall survival was significantly poor in higher miRNAs and lower DACH1 expression in breast cancer patients (p<0.002). Conclusion: DACH1 down-regulation may be associated with a poor breast cancer prognosis. The DACH1 down-regulation may be due to epigenetic regulations such as promoter methylation, especially in triple-negative cases. Other factors, such as micro-RNAs (miR-217, miR-6807-3p, and miR-552), may also have an impact. The elevated expression of miR-217, miR-6807-3p, and miR-552, maybe candidates as possible poor prognostic biomarkers in breast cancer management for further consideration.

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