Membrane lipids from gut microbiome-associated bacteria as structural and signalling molecules

Ryan, Eileen; Joyce, Susan A.; Clarke, David J.

doi:10.1099/mic.0.001315

Cited by 11 publications

(10 citation statements)

References 105 publications

(224 reference statements)

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“…Bacteria synthesize lipids necessary for key cell functions including formation of the cellular envelope [38, 41]. Production of these lipids is mediated by a cascade of enzymes [42].…”

Section: Resultsmentioning

confidence: 99%

“…It is widely accepted that bacterial lipids can be recognized by adaptive T and NK cells during antigen binding on the CD1 receptor [61]. Additionally, studies indicate they can be recognized by other eukaryotic and mammalian receptors including GPCRs [41, 62]. In the gut, short chain fatty acids (SCFAs) generated by bacterial fermentation can bind GPCRs to induce chemokine and cytokine production [63, 64].…”

Section: Discussionmentioning

confidence: 99%

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Staphylococcus aureus lipid factors modulate melanoma clustering and invasion in zebrafish

Giese,

Ramakrishnan,

Steenberge

et al. 2024

Preprint

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The microbiome can influence cancer development and progression. However, less is known about the role of the skin microbiota in melanoma. Here, we take advantage of a zebrafish melanoma model to probe the effects of Staphylococcus aureus on melanoma invasion. We find that S. aureus produces factors that enhance melanoma invasion and dissemination in zebrafish larvae. We used a published in vitro 3D cluster formation assay which correlates increased clustering with tumor invasion. S. aureus supernatant increased clustering of melanoma cells which was abrogated by use of a Rho-Kinase inhibitor, implicating a role for Rho-GTPases. The melanoma clustering response was specific to S. aureus and not other staphylococcal species, including S. epidermidis. Our findings suggest that S. aureus may promote melanoma clustering and invasion via lipids generated by the lipase SAL2 (gehB). Taken together, these findings suggest that specific bacterial products mediate melanoma invasive migration in zebrafish.

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“…Bacteria synthesize lipids necessary for key cell functions including formation of the cellular envelope [38, 41]. Production of these lipids is mediated by a cascade of enzymes [42].…”

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Staphylococcus aureus lipid factors modulate melanoma clustering and invasion in zebrafish

Giese,

Ramakrishnan,

Steenberge

et al. 2024

Preprint

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“…Sulfonolipids are characterized by the replacement of serine in the sphingolipid base by the sulfonic acid capnine generating sulfobacins (monohydroxy) and sulfocristamides (di-hydroxy) [ 118 ]. These lipids are required for gliding motility and demonstrate pro-inflammatory and cytotoxic activities [ 118 ]. Both C. glutamicum and M. bovis were found to possess these highly charged sphingolipids ( Supplementary Table S1 ).…”

Section: Resultsmentioning

confidence: 99%

Construction of a Bacterial Lipidomics Analytical Platform: Pilot Validation with Bovine Paratuberculosis Serum

Wood

Erol

2023

Metabolites

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Lipidomics analyses of bacteria offer the potential to detect and monitor infections in a host since many bacterial lipids are not present in mammals. To evaluate this omics approach, we first built a database of bacterial lipids for representative Gram-positive and Gram-negative bacteria. Our lipidomics analysis of the reference bacteria involved high-resolution mass spectrometry and electrospray ionization with less than a 1.0 ppm mass error. The lipidomics profiles of bacterial cultures clearly distinguished between Gram-positive and Gram-negative bacteria. In the case of bovine paratuberculosis (PTB) serum, we monitored two unique bacterial lipids that we also monitored in Mycobacterium avian subspecies PTB. These were PDIM-B C82, a phthiodiolone dimycocerosate, and the trehalose monomycolate hTMM 28:1, constituents of the bacterial cell envelope in mycolic-containing bacteria. The next step will be to determine if lipidomics can detect subclinical PTB infections which can last 2-to-4 years in bovine PTB. Our data further suggest that it will be worthwhile to continue building our bacterial lipidomics database and investigate the further utility of this approach in other infections of veterinary and human clinical interest.

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“…38 Several membrane lipids from gut microbiome-associated bacteria serve as structural and signaling molecules. 39 Bacterial products (e.g., lipopolysaccharides) are recognized by and activate mast cells. 40 In a microbiota-humanized mouse model, transplantation of microbiota from IBS patients but not from healthy controls was accompanied by attraction and activation of mast cells and increased colocalization of mast cells with nerves.…”

Section: Discussionmentioning

confidence: 99%

Small intestinal bacterial overgrowth in diabetic gastroenteropathy

Varma,

Chakraborty,

Burton

et al. 2024

Neurogastroenterology Motil

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BackgroundAlthough diabetic gastroenteropathy (DGE) is associated with small intestinal bacterial overgrowth (SIBO), most studies have evaluated SIBO with a hydrogen breath test, which may be affected by altered transit in DGE. The risk factors for the consequences of SIBO in DGE are poorly understood. We aimed to evaluate the prevalence of, risk factors for, and gastrointestinal symptoms associated with SIBO in patients with DGE.MethodsIn 75 patients with DGE and dyspepsia, we tested for SIBO (≥105 colony forming units /mL of aerobic and/or anaerobic bacteria in a duodenal aspirate) and assessed gastric emptying (GE) of solids, symptoms during a GE study and during an enteral lipid challenge (300 kcal/2 h), and daily symptoms with a Gastroparesis Cardinal Symptom Index diary for 2 weeks. Symptoms and GE were compared in patients with versus without SIBO.Key ResultsOf 75 patients, 34 (45%) had SIBO, which was not associated with the use of proton pump inhibitors, daily symptoms, GE, or symptoms during a GE study. During enteral lipid challenge, severe nausea (p = 0.006), fullness (p = 0.02) and bloating (p = 0.009) were each associated with SIBO. Twenty patients (59%) with versus 13 (32%) without SIBO had at least one severe symptom during the lipid challenge (p = 0.006).Conclusions & InferencesAmong patients with DGE 45% had SIBO, which was associated with symptoms during enteral lipid challenge but not with delayed GE, symptoms during a GE study, or daily symptoms. Perhaps bacterial products and even fatty acids are recognized by and activate mast cells that drive the increased lipid sensitivity in SIBO.

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Membrane lipids from gut microbiome-associated bacteria as structural and signalling molecules

Cited by 11 publications

References 105 publications

Staphylococcus aureus lipid factors modulate melanoma clustering and invasion in zebrafish

Staphylococcus aureus lipid factors modulate melanoma clustering and invasion in zebrafish

Construction of a Bacterial Lipidomics Analytical Platform: Pilot Validation with Bovine Paratuberculosis Serum

Small intestinal bacterial overgrowth in diabetic gastroenteropathy

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