Membrane lipid perturbation modifies the set point of the temperature of heat shock response in yeast.

Carratù, Luisella; Franceschelli, Silvia; Pardini, Celia; Kobayashi, George S.; Horváth, Ibolya; Vı́gh, László; Maresca, Bruno

doi:10.1073/pnas.93.9.3870

Cited by 180 publications

(168 citation statements)

References 49 publications

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“…Because elevated temperatures are a decisive environmental cue to trigger hyphal morphogenesis in C. albicans, we speculated that the state of its membrane fluidity could act as a cellular 'thermometer' signalling directly to morphogenetic pathways. It has indeed been reported that stress responses and in particular the heat-shock responses in yeasts are activated by membrane perturbations (Carratu et al, 1996;Moskvina et al, 1999). Lowering OLE1 expression in C. albicans indeed led to decreased membrane fluidity, as expected for a direct role of membrane fluidity in adjusting the set point of temperature induction of hyphal growth.…”

Section: Discussionmentioning

confidence: 86%

“…During a heat shock such membrane-induced events could contribute to the complete set of stress responses, which alternatively are activated by protein unfolding (Ananthan et al, 1986;Torok et al, 1997). In yeast an increase in levels of saturated fatty acids within membrane lipids lowered the response to heat shock (Carratu et al, 1996). Similarly, artificially increasing or lowering membrane fluidity in cyanobacteria lowered and, respectively, increased the set point of the heat-shock response (Horvath et al, 1998;Wada et al, 1990;Vigh et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

“…High levels of unsaturated fatty acids, low amounts of sterols in eukaryotic membranes and high temperatures increase fluidity (Carratu et al, 1996;Chatterjee et al, 1997;Horvath et al, 1998). It has been proposed that changes in the physical state of membranes are directly sensed and transmitted by specific signalling pathways triggering protective stress responses (Moskvina et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

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Dosage-dependent functions of fatty acid desaturase Ole1p in growth and morphogenesis of Candida albicans

et al. 2004

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Conditions in the infected human host trigger virulence attributes of the fungal pathogen Candida albicans. Specific inducers and elevated temperatures lead to hyphal development or regulate chlamydospore development. To explore if these processes are affected by membrane lipids, an investigation of the functions of the Ole1 fatty acid desaturase (stearoyl-CoA desaturase) in C. albicans, which synthesizes oleic acid, was undertaken. A conditional strain expressing OLE1 from the regulatable MET3 promoter was unable to grow in repressing conditions, indicating that OLE1 is an essential gene. In contrast, a mutant lacking both alleles of OLE2, encoding a Ole1p homologue, was viable and had no apparent phenotypes. Partial repression of MET3p-OLE1 slightly lowered oleic acid levels and decreased membrane fluidity; these conditions permitted growth in the yeast form, but prevented hyphal development in aerobic conditions and blocked the formation of chlamydospores. In contrast, in hypoxic conditions, which trigger an alternative morphogenetic pathway, hyphal morphogenesis was unaffected. Because aerobic morphogenetic signalling and oleic acid biosynthesis require oxygen, it is proposed that oleic acid may function as a sensor activating specific morphogenetic pathways in normoxic conditions.

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Section: Discussionmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Dosage-dependent functions of fatty acid desaturase Ole1p in growth and morphogenesis of Candida albicans

et al. 2004

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“…By contrast, mild heat stress is not coupled with protein denaturation, and a hitherto unidentified change in the plasma membrane organization and/or composition has been suggested to act as a cellular thermosensor (4,5,25). BA has been shown to up-regulate heat shock genes in Escherichia coli (11), cyanobacteria (3), yeast cells (26), plant cells (27), and mammalian cells (12). Opposite changes in membrane fluidity mimic cold and heat stress activation of distinct MAPK pathways (28).…”

Section: Discussionmentioning

confidence: 99%

Hyperfluidization-coupled membrane microdomain reorganization is linked to activation of the heat shock response in a murine melanoma cell line

Nagy

Balogi

Gombos

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

104

126

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Targeting of the Hsp function in tumor cells is currently being assessed as potential anticancer therapy. An improved understanding of the molecular signals that trigger or attenuate the stress protein response is essential for advances to be made in this field. The present study provides evidence that the membrane fluidizer benzyl alcohol (BA), a documented nondenaturant, acts as a chaperone inducer in B16(F10) melanoma cells. It is demonstrated that this effect relies basically on heat shock transcription factor 1 (HSF1) activation. Under the conditions tested, the BA-induced Hsp response involves the up-regulation of a subset of hsp genes. It is shown that the same level of membrane fluidization (estimated in the core membrane region) attained with the closely analogous phenethyl alcohol (PhA) does not generate a stress protein signal. BA, at a concentration that activates heat shock genes, exerts a profound effect on the melting of raft-like cholesterolsphingomyelin domains in vitro, whereas PhA, at a concentration equipotent with BA in membrane fluidization, has no such effect. Furthermore, through the in vivo labeling of melanoma cells with a fluorescein labeled probe that inserts into the cholesterol-rich membrane domains [fluorescein ester of polyethylene glycol-derivatized cholesterol (fPEG-Chol)], we found that, similarly to heat stress per se, BA, but not PhA, initiates profound alterations in the plasma membrane microdomain structure. We suggest that, apart from membrane hyperfluidization in the deep hydrophobic region, a distinct reorganization of cholesterol-rich microdomains may also be required for the generation and transmission of stress signals to activate hsp genes. molecular chaperones ͉ stress signaling ͉ membrane defects ͉ rafts ͉ cancer therapy

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“…It seems likely that this CER is due to a common effect on cells when exposed to almost any change that affects permeability of the cell wall or integrity of protein structure. The permeability of the cell wall increases in response to heat, spheroplasting, and ethanol, and the consequences of increased permeability may contribute to a common response (Adams and Gross, 1991;Carratù et al, 1996). Diverse changes in environmental conditions may generally induce molecular chaperones because of their effects on the structural integrity of proteins, and this, in turn, may require substantial increases in energy production because many molecular chaperones use the energy of ATP hydrolysis (Lindquist, 1992).…”

Section: Cermentioning

confidence: 99%

Remodeling of Yeast Genome Expression in Response to Environmental Changes

Causton

Ren

Koh

et al. 2001

MBoC

1,211

1,257

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We used genome-wide expression analysis to explore how gene expression in Saccharomyces cerevisiae is remodeled in response to various changes in extracellular environment, including changes in temperature, oxidation, nutrients, pH, and osmolarity. The results demonstrate that more than half of the genome is involved in various responses to environmental change and identify the global set of genes induced and repressed by each condition. These data implicate a substantial number of previously uncharacterized genes in these responses and reveal a signature common to environmental responses that involves ϳ10% of yeast genes. The results of expression analysis with MSN2/MSN4 mutants support the model that the Msn2/Msn4 activators induce the common response to environmental change. These results provide a global description of the transcriptional response to environmental change and extend our understanding of the role of activators in effecting this response.

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Membrane lipid perturbation modifies the set point of the temperature of heat shock response in yeast.

Cited by 180 publications

References 49 publications

Dosage-dependent functions of fatty acid desaturase Ole1p in growth and morphogenesis of Candida albicans

Dosage-dependent functions of fatty acid desaturase Ole1p in growth and morphogenesis of Candida albicans

Hyperfluidization-coupled membrane microdomain reorganization is linked to activation of the heat shock response in a murine melanoma cell line

Remodeling of Yeast Genome Expression in Response to Environmental Changes

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