Membrane ion-exchange chromatography for process-scale antibody purification

Knudsen, Heather L.; Fahrner, Robert; Xu, Yuan; Norling, Lenore A.; Blank, G.

doi:10.1016/s0021-9673(00)01041-4

Cited by 216 publications

(118 citation statements)

References 22 publications

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“…For membrane chromatography, binding sites are located along the through pores rather than nestled within long diffusive pores. 78 Accordingly, mass transfer of biomolecules to binding sites depends on convection instead of diffusion and binding capacities of membrane chromatography are largely independent of flow rate.…”

Section: Membrane and Filtration Technologymentioning

confidence: 99%

“…79,80 Among the adsorptive membranes commercially available, the Q membrane adsorber has attracted a lot of industry interest, especially in mAb purification processes. 78,81,82 Commercially available Q membranes include Intercept TM (Millipore), Mustang ® (Pall) and Sartobind ® (Sartorius). Similar to conventional bed chromatography, Q membranes are normally exploited as polishing steps in flow-through mode to remove trace amounts of impurities.…”

Section: Membrane and Filtration Technologymentioning

confidence: 99%

“…It results a lower filtration throughput with the Lot 3 in-process pool. 78,83 Flocculation is a method that has been widely used in other fields and is gathering interest in the recovery of proteins from mammalian cell culture. One of its most tangible advantages is the reduction of membrane surface area in harvest depth filtration.…”

Section: Perspectives On the Futurementioning

confidence: 99%

See 2 more Smart Citations

Recovery and purification process development for monoclonal antibody production

Liu

Winter

et al. 2010

mAbs

452

411

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Section: Membrane and Filtration Technologymentioning

confidence: 99%

Section: Membrane and Filtration Technologymentioning

confidence: 99%

Section: Perspectives On the Futurementioning

confidence: 99%

See 1 more Smart Citation

Recovery and purification process development for monoclonal antibody production

Liu

Winter

et al. 2010

mAbs

452

411

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“…Lee et al (1995) have described the biochemical engineering fundamentals of rotating disk dynamic filtration systems as a basis for scale-up. Membranes as a basis for adsorbents have also been the subject of scale-up studies (Knudsen et al, 2001) and a scaledown model for a membrane-based adsorbent as an alternative to chromatography has recently been described (Zhou et al, 2006). Costa et al (2000) have conducted a study of scale-up of a recombinant enzymic protein by whole broth extraction with a polyethyleneglycol-phosphate aqueous two phase system.…”

Section: Recent Scale-down Studiesmentioning

confidence: 99%

Micro biochemical engineering to accelerate the design of industrial‐scale downstream processes for biopharmaceutical proteins

Titchener‐Hooker

Dunnill

Hoare

2008

Biotech & Bioengineering

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The article examines how a small set of easily implemented micro biochemical engineering procedures combined with regime analysis and bioprocess models can be used to predict industrial scale performance of biopharmaceutical protein downstream processing. This approach has been worked on in many of our studies of individual operations over the last 10 years and allows preliminary evaluation to be conducted much earlier in the development pathway because of lower costs. It then permits the later large scale trials to be more highly focused. This means that the risk of delays during bioprocess development and of product launch are reduced. Here we draw the outcomes of this research together and illustrate its use in a set of typical operations; cell rupture, centrifugation, filtration, precipitation, expanded bed adsorption, chromatography and for common sources, E. coli, two yeasts and mammalian cells (GS-NSO). The general approach to establishing this method for other operations is summarized and new developments outlined. The technique is placed against the background of the scale-down methods that preceded it and complementary ones that are being examined in parallel. The article concludes with a discussion of the advantages and limitations of the micro biochemical engineering approach versus other methods.

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“…matrices. It has been used for the purification of monoclonal antibody [24], serum antibody [25], 2 .2. Fish serum albumin [26], enzymes [27], etc.…”

mentioning

confidence: 99%

Membrane chromatographic method for the rapid purification of vitellogenin from fish plasma

Shi

Shao

Jiang

et al. 2003

Journal of Chromatography B

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A membrane chromatographic method was developed for the rapid purification of vitellogenin (Vtg) from the plasma of 17b-estradiol induced loach (Misgurnus angaillicaud atus) and carp (Cyprinus carpio). The time required for the proposed procedure is less then 10 min at a flow-rate of 5 ml / min of the mobile phase, and 0.5 ml of fish plasma could be separated in one cycle. Multistep gradient elution was more suitable for the separation than linear gradient elution. Under optimized conditions, a single Vtg peak can be obtained and its identity was confirmed by SDS-PAGE and gel-permeation chromatography assessment. This method is rapid and easy to operate compared to conventional HPLC and FPLC columns for Vtg separation. 

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Membrane ion-exchange chromatography for process-scale antibody purification

Cited by 216 publications

References 22 publications

Recovery and purification process development for monoclonal antibody production

Recovery and purification process development for monoclonal antibody production

Micro biochemical engineering to accelerate the design of industrial‐scale downstream processes for biopharmaceutical proteins

Membrane chromatographic method for the rapid purification of vitellogenin from fish plasma

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