Membrane Interactions of the Mason-Pfizer Monkey Virus Matrix Protein and Its Budding Deficient Mutants

Kroupa, Tomáš; Langerová, Hana; Doležal, Michal; Prchal, Jan; Spiwok, Vojtěch; Hunter, Eric; Rumlová, Michaela; Hrabal, Richard; Ruml, Tomáš

doi:10.1016/j.jmb.2016.10.010

Cited by 4 publications

(9 citation statements)

References 61 publications

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“…Our results are in good agreement with the study performed by Kroupa et al (41), in which the membrane interaction and the affinity of M-PMV and HIV-1 MAs to differently composed liposomes was also inspected. Via a liposome pelleting assay, Kroupa et al (41) found that HIV-1 MA efficiently interacted with PC/PS liposomes (;20% of the HIV-1 MA was bound to PC/PS liposomes) with almost half-effectivity as compared with PI4,5P 2 -containing liposomes (more than 40% of the HIV-1 MA was bound to PI4,5P 2 liposomes). The more significant difference between the HIV-1 MA binding to PC/PS and PI4,5P 2 in their data is probably caused by the higher proportion of PS in the PI4,5P 2 -containing liposomes used by them (30 mol % in contrast to our 5 mol %).…”

Section: Liposome Binding Of Hiv-1 and Mmtv Massupporting

confidence: 93%

“…In our previous study, we found that ;50% of the M-PMV MA was bound to PI4,5P 2 -containing liposomes (39). However, the concentration of the phospholipids used in the mixture of M-PMV MA and liposomes was almost five times higher than that used by Kroupa et al (41) and twice as high as in our current study to obtain a sufficient amount of protein bound to the liposomes for subsequent mapping experiments.…”

Section: Liposome Binding Of Hiv-1 and Mmtv Mascontrasting

confidence: 62%

“…Additionally, only 26% of the MMTV MA remained bound to PC/PS liposomes. The lower affinity of the M-PMV MA, a D-type retrovirus, to liposomes has previously been observed by Kroupa et al (41), where only 5% of the M-PMV MA was bound to PI4,5P 2 -containing liposomes in contrast to more than 40% of the HIV-1 MA bound to the same liposomes. In our previous study, we found that ;50% of the M-PMV MA was bound to PI4,5P 2 -containing liposomes (39).…”

Section: Liposome Binding Of Hiv-1 and Mmtv Massupporting

confidence: 61%

“…In fact, the modification of this residue was detected only once in three independent experiments (see Table S2 for detailed results). On the other hand, residues Tyr 36 and Lys 41 were not modified in the free state of the MMTV MA, and residue Lys 65 was not detected at all. Residue Tyr 36 is buried in the protein core, where it is oriented toward the cavity at the N terminus of the MMTV MA, which is enriched in hydrophobic residues and expected to mediate hydrophobic interactions with myristoyl.…”

Section: The Surface Mapping Of Hiv-1 and Mmtv Masmentioning

confidence: 80%

“…Residue Tyr 36 is buried in the protein core, where it is oriented toward the cavity at the N terminus of the MMTV MA, which is enriched in hydrophobic residues and expected to mediate hydrophobic interactions with myristoyl. Additionally, we suppose that the reasons why residues Lys 41 and Lys 65 were not modified or even detected are related to the primary structure of the MMTV MA. Residue Lys 41 is located in the part of the sequence lacking other lysines or arginines.…”

Section: The Surface Mapping Of Hiv-1 and Mmtv Masmentioning

confidence: 99%

See 4 more Smart Citations

Differences and commonalities in plasma membrane recruitment of the two morphogenetically distinct retroviruses HIV-1 and MMTV

Junková

Pleskot

Prchal

et al. 2020

Journal of Biological Chemistry

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Retroviral Gag polyproteins are targeted to the inner leaflet of the plasma membrane through their N-terminal matrix (MA) domain. Because retroviruses of different morphogenetic types assemble their immature particles in distinct regions of the host cell, the mechanism of MA-mediated plasma membrane targeting differs among distinct retroviral morphogenetic types. Here, we focused on possible mechanistic differences of the MA-mediated plasma membrane targeting of the B-type mouse mammary tumor virus (MMTV) and C-type HIV-1, which assemble in the cytoplasm and at the plasma membrane, respectively. Molecular dynamics simulations, together with surface mapping, indicated that, similarly to HIV-1, MMTV uses a myristic switch to anchor the MA to the membrane and electrostatically interacts with phosphatidylinositol 4,5-bisphosphate to stabilize MA orientation. We observed that the affinity of MMTV MA to the membrane is lower than that of HIV-1 MA, possibly related to their different topologies and the number of basic residues in the highly basic MA region. The latter probably reflects the requirement of C-type retroviruses for tighter membrane binding, essential for assembly, unlike for D/B-type retroviruses, which assemble in the cytoplasm. A comparison of the membrane topology of the HIV-1 MA, using the surface-mapping method and molecular dynamics simulations, revealed that the residues at the HIV-1 MA C terminus help stabilize protein–protein interactions within the HIV-1 MA lattice at the plasma membrane. In summary, HIV-1 and MMTV share common features such as membrane binding of the MA via hydrophobic interactions and exhibit several differences, including lower membrane affinity of MMTV MA.

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Section: Liposome Binding Of Hiv-1 and Mmtv Massupporting

confidence: 93%

Section: Liposome Binding Of Hiv-1 and Mmtv Mascontrasting

confidence: 62%

Section: Liposome Binding Of Hiv-1 and Mmtv Massupporting

confidence: 61%

Section: The Surface Mapping Of Hiv-1 and Mmtv Masmentioning

confidence: 80%

Section: The Surface Mapping Of Hiv-1 and Mmtv Masmentioning

confidence: 99%

See 3 more Smart Citations

Differences and commonalities in plasma membrane recruitment of the two morphogenetically distinct retroviruses HIV-1 and MMTV

Junková

Pleskot

Prchal

et al. 2020

Journal of Biological Chemistry

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Expression, purification, and characterization of biologically active full-length Mason-Pfizer monkey virus (MPMV) Pr78Gag

Pitchai¹,

Ali²,

Pillai³

et al. 2018

Sci Rep

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MPMV precursor polypeptide Pr78Gag orchestrates assembly and packaging of genomic RNA (gRNA) into virus particles. Therefore, we have expressed recombinant full-length Pr78Gag either with or without His6-tag in bacterial as well as eukaryotic cultures and purified the recombinant protein from soluble fractions of the bacterial cultures. The recombinant Pr78Gag protein has the intrinsic ability to assemble in vitro to form virus like particles (VLPs). Consistent with this observation, the recombinant protein could form VLPs in both prokaryotes and eukaryotes. VLPs formed in eukaryotic cells by recombinant Pr78Gag with or without His6-tag can encapsidate MPMV transfer vector RNA, suggesting that the inclusion of the His6-tag to the full-length Pr78Gag did not interfere with its expression or biological function. This study demonstrates the expression and purification of a biologically active, recombinant Pr78Gag, which should pave the way to study RNA-protein interactions involved in the MPMV gRNA packaging process.

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A myristoyl switch at the plasma membrane triggers cleavage and oligomerization of Mason-Pfizer monkey virus matrix protein

Častorálová,

Sýs,

Prchal

et al. 2023

Preprint

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For most retroviruses, including HIV, association with the plasma membrane (PM) promotes the assembly of immature particles, which occurs simultaneously with budding and maturation. In these viruses, maturation is initiated by oligomerization of polyprotein precursors. In contrast, several retroviruses, such as Mason-Pfizer monkey virus (M-PMV), assemble in the cytoplasm into immature particles that are transported across the PM. Therefore, protease activation and specific cleavage must not occur until the preassembled particle interacts with the PM. This interaction is triggered by a bipartite signal consisting of a cluster of basic residues in the matrix (MA) domain of Gag polyprotein and a myristoyl moiety N-terminally attached to MA. Here, we provide evidence that myristoyl exposure from the MA core and its insertion into the PM occurs in M-PMV. By a combination of experimental methods, we show that this results in a structural change at the C-terminus of MA allowing efficient cleavage of MA from the downstream region of Gag. This suggests that, in addition to the known effect of the myristoyl switch of HIV-1 MA on the multimerization state of Gag and particle assembly, the myristoyl switch may have a regulatory role in initiating sequential cleavage of M-PMV Gag in immature particles.

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Membrane Interactions of the Mason-Pfizer Monkey Virus Matrix Protein and Its Budding Deficient Mutants

Cited by 4 publications

References 61 publications

Differences and commonalities in plasma membrane recruitment of the two morphogenetically distinct retroviruses HIV-1 and MMTV

Differences and commonalities in plasma membrane recruitment of the two morphogenetically distinct retroviruses HIV-1 and MMTV

Expression, purification, and characterization of biologically active full-length Mason-Pfizer monkey virus (MPMV) Pr78Gag

A myristoyl switch at the plasma membrane triggers cleavage and oligomerization of Mason-Pfizer monkey virus matrix protein

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