Membrane interactions of mesoporous silica nanoparticles as carriers of antimicrobial peptides

Braun, Katharina; Pochert, Alexander; Lindén, Mika; Davoudi, Mina; Schmidtchen, Artur; Nordström, Randi; Malmsten, Martin

doi:10.1016/j.jcis.2016.05.002

Cited by 148 publications

(107 citation statements)

References 35 publications

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“…Their surface functionalities can be modified to target both planktonic bacteria and biofilms [39]. In recent studies, the utility of MSN for efficient antibiotic delivery [36,40,41] and hybrid antibacterial materials preparation by incorporating antibacterial enzymes [42], peptides [43], metal ions/ particles [44], and polymers (surface modifiers) [45] to MSNs has been reported.…”

Section: Nanomaterials As Antibacterial Delivery Systemsmentioning

confidence: 99%

Current Approaches for Exploration of Nanoparticles as Antibacterial Agents

Karaman¹,

Manner²,

Fallarero³

et al. 2017

Antibacterial Agents

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The ascending anxiety regarding antimicrobial resistance as well as the recalcitrant nature of biofilm-associated infections call for the development of alternative strategies to treat bacterial diseases. Nanoparticles have been considered as one of the emerging and promising platforms in this respect. Their unique physical and chemical properties may lead to fine-tuned interactions between them and bacteria. In this chapter, we aim to provide an overview on the use of nanoparticles as antimicrobial agents. Both antibacterial and anti-biofilm activities of nanoparticles and their current approaches will be reviewed. The in vitro methods that are used to evaluate the potency of nanoparticles as antimicrobial agents will be discussed in detail.

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Section: Nanomaterials As Antibacterial Delivery Systemsmentioning

confidence: 99%

Current Approaches for Exploration of Nanoparticles as Antibacterial Agents

Karaman¹,

Manner²,

Fallarero³

et al. 2017

Antibacterial Agents

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“…[39] The result of this One of the most used procedures to obtain colloidal mesoporous silica is the Stöber method. [40][41][42][43] This method used several bases (KOH, NaOH, ammonia, triethanolamine) and a silica source (tetraetylortosilicate, TEOS) to prepare mesoporous silica nanoparticles with diameters in the 20-150 nm interval.…”

Section: Scheme 12mentioning

confidence: 99%

“…[41] They tested nine dipeptide systems with different amino acids and showed that three of them were stable at physiological pH. Then these self-assembled FMOC-dipeptides were used for proliferation and retention of phenotype bovine chondrocytes, and they showed good ability to support proliferation of these cells.…”

Section: Biocompatibility-cytotoxicitymentioning

confidence: 99%

“…[36,37} In this scenario, MSNs have been used to encapsulate and deliver proteins [38][39][40] but its use as inorganic carriers for the controlled release of small peptides has been barely explored. [41] Scheme 1. Representation of the design and mechanism of action of MSNs S4 loaded with C9h peptide in the presence of intracellular proteases (up) and mechanism of action of C9h blocking PP2Acα-caspase 9 interaction (down).…”

Section: Chemicalsmentioning

confidence: 99%

“…[39,40] Despite most B-NHL patients initially respond to chemotherapy, with about 40-50% complete responses, many of them undergo relapse. [41] Drug resistance in relapsed lymphomas is partly acquired by homing lymphoma cells to bone marrow microenvironment where interaction with stromal cells stimulates their survival and confers chemoresistance. [42] This interaction is mainly regulated by chemokine receptor CXCR4, which is expressed in the membrane of lymphoma cells and is activated by the chemokine CXCL12.…”

Section: Introductionmentioning

confidence: 99%

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Nanotechnology and supramolecular chemistry in controlled release and molecular recognition proceses for biomedical applications"

Paredes¹

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CERTIFY:That the work "Nanotechnology and supramolecular chemistry in controlled release and molecular recognition processes for biomedical applications" has been AgradecimientosComo en cualquier carrera de fondo, lo importante de llegar a meta es todo lo que aprendes en el camino. Llegados a este punto, donde me encuentro en la recta final para cruzar la línea de meta, es importante echar la vista atrás y agradecer a todas esas personas que dia a dia te ayudan a continuar caminando, a no detenerte y a afrontar los distintos obstáculos que te encuentras.En primer lugar, agradecer a mi director, el Profesor Ramón Matinez Mañez por darme la oportunidad de realizar la tesis doctoral en su grupo de investigación. Gracias por confiar en mi desde el primer momento, siempre recordaré la entrevista en la que me comentabas que realizaría ensayos en células porque la doctora que se ocupa de ellos se marchaba en breve y yo te contesté, con total sinceridad, que no tenía ningún conocimiento de cultivos celulares pero que aprendería sin problemas (en ese momento en mi cabeza pensaba : "y yo que estudié dibujo técnico en lugar de biología en Bachillerato…mare meua!"). Gracias por darme la oportunidad de crecer "químicamente", por la libertad e independencia que me has dado durante este periodo ya que gracias a ello he aprendido a superar los obstáculos y problemas que surgen, a "buscarme las castañas".Como no, agradecer a Félix Sancenón por ser mi segundo director, por ser el filtro de todos los papers, la persona que siempre está disponible para responder tus dudas experimentales, para corregirte lo que necesites y siempre con una eficiencia difícil de mejorar. Considero que todo el trabajo realizado ha sido posible gracias a tu ayuda y a tus consejos y positividad. Haces las cosas mucho más fáciles, al principio, durante y ahora en la recta final, ayudándome con todos los trámites de la tesis.También agradecer la ayuda prestada a Loles, Jose Vicente, Jose Ramón Murguía y Luis Villaescusa (y sus clases y conversaciones en la sala del Rayos X). AgradecimientosGracias a Quique y Eva, por todos los trámites que nos facilitais, por solucionar los lios de compras en los que nos metemos a veces y por vuestra eficiencia.Muchisimas gracias a Mar Orzaez y Enrique Pérez Payá, por todo lo que me habéis enseñado y por la paciencia que tuvisteis conmigo en los inicios de la tesis.Gracias por aceptarme en vuestro laboratorio y hacer que fuera una más en él.Gracias a Maria José Alcaraz y a su laboratorio, en especial a Carmen Terencio.Gracias por lo que me has enseñado, por como me has tratado, de tú a tú y por la confianza que has puesto en mi. Gracias a Reynaldo por las colaboraciones que hemos realizado y el trabajo en equipo que tan bien organiza.Inifinitas gracias a Eduardo Ruiz, por recibirme con los brazos abiertos en su laboratorio de Dublin. "Una actitud positiva causa una reacción en cadena de pensamientos, sucesos y resultados positivos". Esta frase te define a la perfección.Durante el tiempo que estuvimos trabajando juntos des...

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Controlled Release of LL‐37‐Derived Synthetic Antimicrobial and Anti‐Biofilm Peptides SAAP‐145 and SAAP‐276 Prevents Experimental Biomaterial‐Associated Staphylococcus aureus Infection

Riool

Breij

Boer

et al. 2017

Adv Funct Materials

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The present study aims to develop an implant coating releasing novel antimicrobial agents to prevent biomaterial-associated infections. The LL-37-derived synthetic antimicrobial and anti-biofilm peptides (SAAP)-145 and SAAP-276 exhibit potent bactericidal and anti-biofilm activities against clinical and multidrug-resistant Staphylococcus aureus strains by rapid membrane permeabilization, without inducing resistance. Injection of SAAP-145, but not SAAP-276, along subcutaneous implants in mice reduces S. aureus implant colonization by approximately 2 log, but does not reduce bacterial numbers in surrounding tissue. To improve their efficacy, SAAP-145 and SAAP-276 are incorporated in a polymer-lipid encapsulation matrix (PLEX) coating, providing a constant release of 0.6% daily up to 30 d after an initial burst release of >50%. In a murine model for biomaterial-associated infection, SAAP-145-PLEX and SAAP-276-PLEX coatings significantly reduce the number of culture positive implants and show ≥3.5 and ≥1.5 log lower S. aureus implant and tissue colonization, respectively. Interestingly, these peptide coatings are also highly effective against multidrug-resistant S. aureus, both reducing implant colonization by ≥2 log. SAAP-276-PLEX additionally reduces tissue colonization by 1 log. Together, the peptide-releasing PLEX coatings hold promise for further development as an alternative to coatings releasing conventional antibiotics to prevent biomaterial-associated infections.

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Membrane interactions of mesoporous silica nanoparticles as carriers of antimicrobial peptides

Cited by 148 publications

References 35 publications

Current Approaches for Exploration of Nanoparticles as Antibacterial Agents

Current Approaches for Exploration of Nanoparticles as Antibacterial Agents

Nanotechnology and supramolecular chemistry in controlled release and molecular recognition proceses for biomedical applications"

Controlled Release of LL‐37‐Derived Synthetic Antimicrobial and Anti‐Biofilm Peptides SAAP‐145 and SAAP‐276 Prevents Experimental Biomaterial‐Associated Staphylococcus aureus Infection

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