Membrane inlet ion trap mass spectrometry for the direct measurement of dissolved gases in ecological samples

Cowie, G. A.; Lloyd, David

doi:10.1016/s0167-7012(98)00090-6

Cited by 20 publications

(9 citation statements)

References 12 publications

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“…This clearly shows that measurements of respiration in such waters are still problematic since, at the present time, there is no technique with sensitivity sufficient enough to detect these very low in situ respiration rates. Gattuso et al (2002) have proposed the applications of alternative techniques, which would offer higher oxygen sensitivity and therefore potentially considerably reduce the incubation time such as the use of membrane inlet ion trap mass spectrometry (Cowie and Lloyd 1999), to estimate rates of respiration.…”

Section: Discussionmentioning

confidence: 99%

The use of oxygen microprobes to measure bacterial respiration for determining bacterioplankton growth efficiency

Briand

Pringault

Jacquet

et al. 2004

Limnology & Ocean Methods

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Heterotrophic bacterial growth efficiency (BGE), the ratio between the carbon consumed and the bacterial biomass produced, is a key factor in understanding flows of organic matter in aquatic ecosystems. Methods generally used to estimate bacterial respiration require long incubations (24 to 36 h) to measure significant rates during which nonlinear patterns of oxygen decrease may bias BGE computation. These respiration estimates are generally compared to bacterial production rates determined from radiotracer incorporation from much shorter incubations. The aim of this study was to improve the determination of bacterial respiration to better estimate BGE. For this purpose, we employed oxygen microprobes in predator free (0.6 µm filtered) seawater samples and determined in parallel bacterial abundance. The use of oxygen microprobes allowed us to continuously monitor oxygen concentration during the incubation. Hence, the length of incubation can be adjusted as soon as a significant decrease of oxygen is observed. At the most productive sites, respiration was measurable from the beginning of the incubation and varied with time. In contrast, at the oligotrophic sites, respiration was often detectable only after a lag-phase of 5 to 10 h and remained constant thereafter. BGE was computed from the changes in bacterial abundance observed during the respiration measurements. This way, both processes were determined in similar incubation conditions. In comparison, the use of radiotracer derived bacterial production systematically resulted in an underestimation of BGE.

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Section: Discussionmentioning

confidence: 99%

The use of oxygen microprobes to measure bacterial respiration for determining bacterioplankton growth efficiency

Briand

Pringault

Jacquet

et al. 2004

Limnology & Ocean Methods

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“…denitrifier‐N 2 56), may provide a means of determining the isotope ratio of N 2 O within the soil matrix, with the advantages of rapid sample throughput, small sample size and long‐term continuous monitoring 57. Although this approach has been used to determine N 2 O production in sediments,58, 59 and for CH 4 , CO 2 , NO x , O 2 , Ar and N 2 production in soils,60 it has yet to be developed and utilised for N 2 O source partitioning. Herrmann et al 61.…”

Section: Issues Of Scalementioning

confidence: 99%

A review of stable isotope techniques for N₂O source partitioning in soils: recent progress, remaining challenges and future considerations

Baggs

2008

Rapid Comm Mass Spectrometry

243

208

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Nitrous oxide is produced in soil during several processes, which may occur simultaneously within different micro-sites of the same soil. Stable isotope techniques have a crucial role to play in the attribution of N 2 O emissions to different microbial processes, through estimation (natural abundance, site preference) or quantification (enrichment) of processes based on the 15 N and 18 O signatures of N 2 O determined by isotope ratio mass spectrometry. These approaches have the potential to become even more powerful when linked with recent developments in secondary isotope mass spectrometry, with microbial ecology, and with modelling approaches, enabling sources of N 2 O to be considered at a wide range of scales and related to the underlying microbiology. Such source partitioning of N 2 O is inherently challenging, but is vital to close the N 2 O budget and to better understand controls on the different processes, with a view to developing appropriate management practices for mitigation of N 2 O. In this respect, it is essential that as many of the contributing processes as possible are considered in any study aimed at source attribution, as mitigation strategies for one process may not be appropriate for another. To aid such an approach, here the current state of the art is critically examined, remaining challenges are highlighted, and recommendations are made for future direction.

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“…Although not designed for CP‐MIMS, the pioneering work of the Lloyd group developing miniature probes for ' in situ ' gaseous acceptor phase MIMS helped to inspire the CP‐MIMS probe design presented in this work. Additional influences were the miniature sampling probe designs utilized for in vivo micro‐dialysis .…”

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confidence: 99%

A miniature condensed‐phase membrane introduction mass spectrometry (CP‐MIMS) probe for direct and on‐line measurements of pharmaceuticals and contaminants in small, complex samples

Duncan

Willis

Krogh

et al. 2013

Rapid Comm Mass Spectrometry

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Membrane inlet ion trap mass spectrometry for the direct measurement of dissolved gases in ecological samples

Cited by 20 publications

References 12 publications

The use of oxygen microprobes to measure bacterial respiration for determining bacterioplankton growth efficiency

The use of oxygen microprobes to measure bacterial respiration for determining bacterioplankton growth efficiency

A review of stable isotope techniques for N₂O source partitioning in soils: recent progress, remaining challenges and future considerations

A miniature condensed‐phase membrane introduction mass spectrometry (CP‐MIMS) probe for direct and on‐line measurements of pharmaceuticals and contaminants in small, complex samples

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Membrane inlet ion trap mass spectrometry for the direct measurement of dissolved gases in ecological samples

Cited by 20 publications

References 12 publications

The use of oxygen microprobes to measure bacterial respiration for determining bacterioplankton growth efficiency

The use of oxygen microprobes to measure bacterial respiration for determining bacterioplankton growth efficiency

A review of stable isotope techniques for N2O source partitioning in soils: recent progress, remaining challenges and future considerations

A miniature condensed‐phase membrane introduction mass spectrometry (CP‐MIMS) probe for direct and on‐line measurements of pharmaceuticals and contaminants in small, complex samples

Contact Info

Product

Resources

About

A review of stable isotope techniques for N₂O source partitioning in soils: recent progress, remaining challenges and future considerations