Membrane Initiated Effects of 1α,25-Dihydroxyvitamin D3 in Prostate Cancer Cells: Effects on AP1 and CREB Mediated Transcription

Larsson, D. G. Joakim; Jonas, Adele; Bergsten, Niklas; Ståhl, Fredrik; Karlsson, Sandra

doi:10.5772/32908

Current Frontiers and Perspectives in Cell Biology 2012

DOI: 10.5772/32908

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Membrane Initiated Effects of 1α,25-Dihydroxyvitamin D3 in Prostate Cancer Cells: Effects on AP1 and CREB Mediated Transcription

D. G. Joakim Larsson¹,

Adele Jonas²,

Niklas Bergsten³

et al.

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2021

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“…Although prostate cancer etiology is still largely unknown, there is growing evidence that vitamin D 3 exerts anticancer effects (7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17). Most of the vitamin D is obtained from exposure to sunlight.…”

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confidence: 99%

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Inhibition of CYP27B1 and CYP24 Increases the Anti-proliferative Effects of 25-Hydroxyvitamin D₃ in LNCaP Cells

et al. 2021

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Background/Aim: Growing evidence suggests that vitamin D 3 exerts anticancer effects. The present study aimed to evaluate 25-hydroxyvitamin D 3 (25(OH)D 3 ) as a potential endocrine factor regulating proliferation and vitamin D receptor expression in LNCaP prostate cancer cells. Materials and Methods: Cell counting after treatment was utilized to assess the effect of 25(OH)D 3 on cell proliferation. Changes in mRNA expression of the vitamin D receptors, VDR and PDIA3, were evaluated using droplet digital polymerase chain reaction (ddPCR). Results: 25(OH)D 3 inhibited cell proliferation in a dose-and time-dependent manner. The inhibitory effect of 25(OH)D 3 on cell proliferation was potentiated after inhibition of CYP17B1 and CYP24 by genistein, preventing further metabolism of 25(OH)D 3 to 1,25-dihydroxyvitamin D 3 (1,25(OH) 2 D 3 ) and 24,25-dihydroxyvitamin D 3 (24,25(OH) 2 D 3 ). Expression of PDIA3 and VDR mRNA increased after treatment with 25(OH)D 3 , whereas the ratio between PDIA3 and VDR mRNA remained unchanged. Conclusion: 25(OH)D 3 has a direct inhibitory effect on cell proliferation, which is enhanced and accelerated when the metabolism of 25(OH)D 3 to 1,25(OH) 2 D 3 and 24,25(OH) 2 D 3 was inhibited by the CYP17B1 and CYP24 inhibitor genistein. Furthermore, treatment with 25(OH)D 3 increased receptor transcript expression, suggesting an increased VDR stability and sensibility of the treated cells.

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“…24,25(OH) 2 D 3 causes milder hypercalcemia than 1,25(OH) 2 D 3 and therefore appears as a more attractive therapeutic agent. Consequently, it has been suggested that 24hydroxylase may function as an oncogene (15)(16)(17)(21)(22)(23).…”

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confidence: 99%

Inhibition of CYP27B1 and CYP24 Increases the Anti-proliferative Effects of 25-Hydroxyvitamin D₃ in LNCaP Cells

et al. 2021

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Membrane Initiated Effects of 1α,25-Dihydroxyvitamin D3 in Prostate Cancer Cells: Effects on AP1 and CREB Mediated Transcription

Cited by 1 publication

References 28 publications

Inhibition of CYP27B1 and CYP24 Increases the Anti-proliferative Effects of 25-Hydroxyvitamin D₃ in LNCaP Cells

Inhibition of CYP27B1 and CYP24 Increases the Anti-proliferative Effects of 25-Hydroxyvitamin D₃ in LNCaP Cells

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Membrane Initiated Effects of 1α,25-Dihydroxyvitamin D3 in Prostate Cancer Cells: Effects on AP1 and CREB Mediated Transcription

Cited by 1 publication

References 28 publications

Inhibition of CYP27B1 and CYP24 Increases the Anti-proliferative Effects of 25-Hydroxyvitamin D3 in LNCaP Cells

Inhibition of CYP27B1 and CYP24 Increases the Anti-proliferative Effects of 25-Hydroxyvitamin D3 in LNCaP Cells

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Product

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About

Inhibition of CYP27B1 and CYP24 Increases the Anti-proliferative Effects of 25-Hydroxyvitamin D₃ in LNCaP Cells

Inhibition of CYP27B1 and CYP24 Increases the Anti-proliferative Effects of 25-Hydroxyvitamin D₃ in LNCaP Cells