Membrane-initiated actions of thyroid hormones on the male reproductive system

Zamoner, Ariane; Pessoa-Pureur, Regina; Silva, Fátima Regina Mena Barreto

doi:10.1016/j.lfs.2011.04.006

Cited by 21 publications

(18 citation statements)

References 110 publications

(177 reference statements)

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“…Admittedly, in this study we used a short incubation period of testicular slices (8 h) in the presence of T3 in order to observe early, extra nuclear effects of T3 action on steroidogenesis in heat-acclimated rats (Wagner et al 2008, Zamoner et al 2011). However, we were not able to determine any extra nuclear effects of T3 in testes of the studied rats.…”

Section: Discussionmentioning

confidence: 99%

“…In males with decreased plasma thyroid hormone concentration, adverse changes in the morphology of the reproductive tract, suppressed activity of Leydig and Sertoli cells, and in consequence hypospermatogenesis have been demonstrated (Ariyaratne et al 2000, Oner et al 2006, Wagner et al 2008, Auharek et al 2010, Tousson et al 2012). In the testes, T3 may act through genomic or non-genomic mechanisms (Bassett et al 2003, Zamoner et al 2011. The expression of nuclear thyroid hormone receptors (THR) in testes diminishes during the development of males from neonatal up to pubertal stage (Buzzard et al 2000, Jannini et al 2000, leading to their lower expression in adult rats.…”

Section: Introductionmentioning

confidence: 99%

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Effect of neonatal or adult heat acclimation on plasma fT3 level, testicular thyroid receptors expression in male rats and testicular steroidogenesis in vitro in response to triiodothyronine treatment

Kurowicka¹,

Chruściel²,

Zmijewska³

et al. 2016

Polish Journal of Veterinary Sciences

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This study aimed to evaluate the effect of heat acclimation of neonatal and adult rats on their testes response to in vitro treatment with triiodothyronine (T3). Four groups of rats were housed from birth as: 1) control (CR) at 20 C. Blood plasma and both testes were harvested from 90-day old rats. Testicular slices were then submitted to in vitro treatment with T3 (100 ng/ml) for 8 h. Plasma fT3 level was lower in AHA, NHA and DA groups than in CR group. Basal thyroid hormone receptor α1 (Thra1) expression was higher in testes of NHA and DA and β1 receptor (Thrb1) in DA rats vs. other groups. In the in vitro experiment, T3: 1) decreased Thra1 expression in all groups and Thrb1 in DA group, 2) increased Star expression in CR, NHA and DA groups, and Hsd17b3 expression in NHA group, 3) decreased the expression of Cyp11a1 in NHA and DA groups, and Cyp19a1 in all the groups, 4) did not affect the activity of steroidogenic enzymes and steroid secretion (A4, T, E2) in all the groups. These results indicate, that heat acclimation of rats, depending on their age, mainly affects the testicular expression of steroidogenic enzymes in response to short-lasting treatment with T3.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of neonatal or adult heat acclimation on plasma fT3 level, testicular thyroid receptors expression in male rats and testicular steroidogenesis in vitro in response to triiodothyronine treatment

Kurowicka¹,

Chruściel²,

Zmijewska³

et al. 2016

Polish Journal of Veterinary Sciences

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“…Thyroid hormone binds to the binding elements such as integrin αvβ3 located at the plasma membrane or within a cell to exert its effects. These non-gemonic effects include the regulation of ion influxes, kinase signaling pathways, amino acid accumulation, extracellular nucleotide levels, and vimentin phosphorylation via non-receptor protein kinases downstream (10). While T4 is a pro-hormone, it can bind to TRs but with low affinity, and the T4 liganded-TR is less stable versus the T3-liganded-TR.…”

Section: Thyroid Hormone Actionmentioning

confidence: 99%

“…Moreover, it also induces Leydig cell differentiation and stimulates steroidogenesis in the rat testis (6). Several idothyronine deiodinases and thyroid hormone transporters have been identified in the testis (7–10), illustrating that these enzymes and transporters necessary to maintain the homeostasis of thyroid hormone are present in the testis. It is generally accepted that thyroid hormone acts as an important regulator in testis development.…”

Section: Introductionmentioning

confidence: 99%

Thyroid Hormone Function in the Rat Testis

2014

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Thyroid hormones are emerging regulators of testicular function since Sertoli, germ, and Leydig cells are found to express thyroid hormone receptors (TRs). These testicular cells also express deiodinases, which are capable of converting the pro-hormone T4 to the active thyroid hormone T3, or inactivating T3 or T4 to a non-biologically active form. Furthermore, thyroid hormone transporters are also found in the testis. Thus, the testis is equipped with the transporters and the enzymes necessary to maintain the optimal level of thyroid hormone in the seminiferous epithelium, as well as the specific TRs to execute thyroid hormone action in response to different stages of the epithelial cycle of spermatogenesis. Studies using genetic models and/or goitrogens (e.g., propylthiouracil) have illustrated a tight physiological relationship between thyroid hormone and testicular function, in particular, Sertoli cell differentiation status, mitotic activity, gap junction function, and blood–testis barrier assembly. These findings are briefly summarized and discussed herein.

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“…So far, attention on the endocrine activity of chemicals has largely focused on estrogen disruption. An analysis of literature search resistance (Zamoner et al, 2011). the clinical outcomes of such disturbances include arrhythmias, reduced exercise performance, and an increased risk of cardiovascular mortality (Klein and Ojamaa, 2001;Fazio et al, 2004).…”

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confidence: 99%

In vitro pituitary and thyroid cell proliferation assays and their relevance as alternatives to animal testing

Jomaa

2013

ALTEX

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Summary This study investigates the in vitro effect of eleven thyroid-active compounds known to affect pituitary and/or thyroid weights in vivo, using the proliferation of GH3 rat pituitary cells in the so-called "T-screen," and of FRTL-5 rat thyroid cells in a newly developed test denoted "TSH-screen" to gain insight into the relative value of these in vitro proliferation tests for an integrated testing strategy (ITS) for thyroid activity. Pituitary cell proliferation in the T-screen was stimulated by three out of eleven tested compounds, namely thyrotropin releasing hormone (TRH), triiodothyronine (T3) and thyroxine (T4). Of these three compounds, only T4 causes an increase in relative pituitary weight, and thus T4 was the only compound for which the effect in the in vitro assay correlated with a reported in vivo effect. As to the newly developed TSH-screen, two compounds had an effect, namely, thyroid-stimulating hormone (TSH) induced and T4 antagonized FRTL-5 cell proliferation. These effects correlated with in vivo changes induced by these compounds on thyroid weight. Altogether, the results indicate that most of the selected compounds affect pituitary and thyroid weights by modes of action different from a direct thyroid hormone receptor (THR) or TSH receptor (TSHR)-mediated effect, and point to the need for additional in vitro tests for an ITS. Additional analysis of the T-screen revealed a positive correlation between the THR-mediated effects of the tested compounds in vitro and their effects on relative heart weight in vivo, suggesting that the T-screen may directly predict this THR-mediated in vivo adverse effect.

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Membrane-initiated actions of thyroid hormones on the male reproductive system

Cited by 21 publications

References 110 publications

Effect of neonatal or adult heat acclimation on plasma fT3 level, testicular thyroid receptors expression in male rats and testicular steroidogenesis in vitro in response to triiodothyronine treatment

Effect of neonatal or adult heat acclimation on plasma fT3 level, testicular thyroid receptors expression in male rats and testicular steroidogenesis in vitro in response to triiodothyronine treatment

Thyroid Hormone Function in the Rat Testis

In vitro pituitary and thyroid cell proliferation assays and their relevance as alternatives to animal testing

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