Membrane fusion by an RGD-containing sequence from the core protein VP3 of hepatitis A virus and the RGA-analogue: Implications for viral infection

Chávez, Alice Rocío; Pujol, Montserrat; Haro, Isabel; Alsina, M. A.; Cajal, Yolanda

doi:10.1002/1097-0282(200101)58:1<63::aid-bip70>3.0.co;2-l

Cited by 10 publications

(4 citation statements)

References 60 publications

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“…In the absence of vesicles, sP-Bw has a K SV value of about 13 M -1 ; similar or even higher values have been reported for other peptides in solution, such as fusogenic peptides from hepatitis A virus, mellitin, and cyclic antimicrobial hexapeptides . All of the Trp residues of sP-B in solution are accessible to acrylamide ( f ∼ 1), in agreement with the fluorescence emission spectra.…”

Section: Resultssupporting

confidence: 81%

Membrane Association and Contact Formation by a Synthetic Analogue of Polymyxin B and Its Fluorescent Derivatives

et al. 2006

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sP-B is a synthetic analogue of the natural lipopeptide antibiotic polymyxin B (PxB) that maintains the ability of the parent compound to form vesicle-vesicle contacts and induce lipid exchange. Exchange is selective, and only monoanionic phospholipids such as 1-palmitoyl-2-oleoyl-glycero-sn-3-phosphoglycerol (POPG) are transferred, whereas dianionic phospholipids such as 1-palmitoyl-2-oleoyl-glycero-sn-3-phosphate (POPA) are not, as shown by fluorescence experiments based on the excimer/monomer ratio of pyrene-labeled phospholipids. Synthetic fluorescent analogues of sP-B are used to investigate the peptide position and orientation in the intermembrane contacts: sP-Bw, an analogue that contains D-tryptophan (D-Trp) instead of the naturally occurring D-phenylalanine, and sP-Bpy, incorporating a pyrene group at the N-terminus. Tryptophan fluorescence, anisotropy, and quenching measurements performed with sP-Bw indicate that the peptide binds and inserts in anionic vesicles of POPG and POPA. However, significant differences are seen depending on the lipid composition, as also demonstrated by fluorescence resonance energy transfer (FRET) experiments from Trp to 7-nitro-2-1,3-benzoxadiazol (NBD) groups at the interface. Intermolecular FRET using sP-Bw as the donor and sP-Bpy as the acceptor indicates self-association of the peptide, possibly forming dimers, when bound to POPG vesicles at concentrations that induce the vesicle-vesicle contacts.

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Section: Resultssupporting

confidence: 81%

Membrane Association and Contact Formation by a Synthetic Analogue of Polymyxin B and Its Fluorescent Derivatives

et al. 2006

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“…In addition to improving cell uptake, the cRGD peptide may also affect the intracellular process of ligand-equipped polyplexes. Several publications have shown that that RGD motif can destabilize the membrane at low pH and trigger the enhanced endosomal release of adenovirus [57,58]. To investigate the subcellular distribution of fluorescentlabeled siRNA, lysosome compartments were stained with LysoTracker Red, which is a specific marker for acidic organelles.…”

Section: Effect Of Post-pegylation Of C-phda Polyplexes With Peg-crgdmentioning

confidence: 99%

RGD-decorated cholesterol stabilized polyplexes for targeted siRNA delivery to glioblastoma cells

Lou

Connor

Sweeney

et al. 2019

Drug Deliv. and Transl. Res.

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The development of an effective and safe treatment for glioblastoma (GBM) represents a significant challenge in oncology today. Downregulation of key mediators of cell signal transduction by RNA interference is considered a promising treatment strategy but requires efficient, intracellular delivery of siRNA into GBM tumor cells. Here, we describe novel polymeric siRNA nanocarriers functionalized with cRGD peptide that mediates targeted and efficient reporter gene silencing in U87R invasive human GBM cells. The polymer was synthesized via RAFT copolymerization of N-(2-hydroxypropyl)-methacrylamide (HPMA) and N-acryloxysuccinimide (NAS), followed by post-polymerization modification with cholesterol for stabilization, cationic amines for siRNA complexation, and azides for copper-free click chemistry. The novel resultant cationic polymer harboring a terminal cholesterol group, self-assembled with siRNA to yield nanosized polyplexes (~40 nm) with good colloidal stability at physiological ionic strength. Post-modification of the preformed polyplexes with PEG-cRGD end-functionalized with bicyclo[6.1.0]nonyne (BCN) group resulted in enhanced cell uptake and increased luciferase gene silencing in U87R cells, compared to polyplexes lacking cRGD-targeting groups.

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“…Thus, the knowledge of the protein composition of the capsid protein helps in designing proteomic analysis of viral and/or host proteins. Biologically, the knowledge about the composition of capsid protein is important to determine the antigenicity of the capsid proteins …”

Section: Challengesmentioning

confidence: 99%

“…Biologically, the knowledge about the composition of capsid protein is important to determine the antigenicity of the capsid proteins. 93 Astroviruses are small RNA viruses in which the capsid protein composition of the reference strains and several wild isolates of human astroviruses has been analyzed using highresolution polyacrylamide gradient gels. 95 A consistent pattern of three infection-specific proteinssdesignated P1, P2, and P3swas generally observed.…”

Section: Challengesmentioning

confidence: 99%

Proteomic Maps of the Cancer-Associated Infectious Agents

2005

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The number of infectious agents associated with cancer is increasing. There is a need to develop approaches for the early detection of the infected host which might lead to tumor development. Recent advances in proteomic approaches provide that opportunity, and it is now possible to generate proteomic maps of cancer-associated infectious agents. Protein arrays, interaction maps, data archives, and biological assays are being developed to enable efficient and reliable protein identification and functional analysis. Herein, we discuss the current technologies and challenges in the field, and application of protein signatures in cancer detection and prevention.

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Membrane fusion by an RGD-containing sequence from the core protein VP3 of hepatitis A virus and the RGA-analogue: Implications for viral infection

Cited by 10 publications

References 60 publications

Membrane Association and Contact Formation by a Synthetic Analogue of Polymyxin B and Its Fluorescent Derivatives

Membrane Association and Contact Formation by a Synthetic Analogue of Polymyxin B and Its Fluorescent Derivatives

RGD-decorated cholesterol stabilized polyplexes for targeted siRNA delivery to glioblastoma cells

Proteomic Maps of the Cancer-Associated Infectious Agents

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