Membrane fusion and molecular segregation in phospholipid vesicles

Papahadjopoulos, D.; Poste, George; Schaeffer, Barry E.; Vail, William J.

doi:10.1016/0005-2736(74)90175-8

Cited by 428 publications

(161 citation statements)

References 32 publications

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“…This hypothesis is further supported by the sensitivity of fusion against cholesterol (Table IV) and lysophosphatidylcholine [24], agents which interfere with phase transitions and phase separations [58,59].…”

Section: Discussionmentioning

confidence: 91%

Fusion of isolated myoblast plasma membranes. An approach to the mechanism

Dahl

Schudt

Gratzl

1978

Biochimica et Biophysica Acta (BBA) - Biomembranes

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Summary 105Fusion of plasma membranes isolated from myoblasts grown in culture has been investigated.1. Membrane fusion was specifically dependent on Ca 2 + at physiological concentrations. However, at higher concentrations of cations, fusion could be triggered not only by Ca2+, but by Mg2+ and Sr 2 + as weH.2. The amount of fusion was directly proportional to temperature. 3. Fusion was found to depend on the state of maturation of the my oblast membranes.4. Experiments with chemically and enzymatically modified mem branes and with membranes derived from myoblasts grown in the presence of inhibitors of protein biosynthesis suggest the participation of proteinaceous membrane components in the fusion mechanism.

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Section: Discussionmentioning

confidence: 91%

Fusion of isolated myoblast plasma membranes. An approach to the mechanism

Dahl

Schudt

Gratzl

1978

Biochimica et Biophysica Acta (BBA) - Biomembranes

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“…The emission spectrum was not influenced by the presence of phospholipid vesicles or of Triton X-100. In the fusion assay described here vesicles were used consisting of equimolar amounts of PC and PS because a variety of-methods [9,16,24,26] have demonstrated that such vesicles tend to fuse under proper experiments conditions. When free trypsin was added to substrate-containing vesicles a very slow rate of fluorescence increase was measured.…”

Section: Resultsmentioning

confidence: 99%

Fusion of phospholipid vesicles containing a trypsin‐sensitive fluorogenic substrate and trypsin

et al. 1979

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“…Phosphatidylserine is the component most likely to be affected by the conditions used, as the pH and the concentration of ions can strongly influence its physicochemical properties via effects on the charged polar head group [50]. Both a low pH [51] and the presence of calcium [ 52] have been shown to raise the phase transition temperature of phosphatidylserine dramatically. Calcium has been shown to induce lateral [53,54] and structural [51] phase separations in mixtures of phosphatidylserine and phosphatidylcholine.…”

Section: Discussionmentioning

confidence: 99%

The lateral distribution of intramembrane particles in the erythrocyte membrane and recombinant vesicles

Gerritsen

Verkleij

Deenen

1979

Biochimica et Biophysica Acta (BBA) - Biomembranes

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SummaryTriton X-100 (in concentrations which did not cause a significant solubilization of membrane material) caused aggregation of the intramembrane particles of human erythrocyte ghosts.Ghosts from which the extrinsic proteins had been removed by alkali treatment showed a temperature-induced aggregation of the particles. With virtually no spectrin present, the particles in these stripped ghosts could still be aggregated by manipulations with ionic strength and pH, or by the addition of calcium.Recombinant vesicles were made from a Triton X-100 extract and a mixture of phospholipids with a composition which resembled that of the inner monolayer of erythrocyte membrane. In these recombinants the same manipulations with ionic strength and pH and the addition of calcium caused a rearrangement of the particles, resulting in the appearance of particle-free areas. In recombinants prepared from a Triton X-100 extract and egg phosphatidylcholine the lateral distribution of the particles was not altered by these manipulations.It is concluded that in the erythrocyte membrane the intramembrane particles can be aggregated by effects of external agents on lipid components. In this light the role of spectrin in stabilizing the membrane by interactions with lipids in the inner monolayer is discussed.

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Membrane fusion and molecular segregation in phospholipid vesicles

Cited by 428 publications

References 32 publications

Fusion of isolated myoblast plasma membranes. An approach to the mechanism

Fusion of isolated myoblast plasma membranes. An approach to the mechanism

Fusion of phospholipid vesicles containing a trypsin‐sensitive fluorogenic substrate and trypsin

The lateral distribution of intramembrane particles in the erythrocyte membrane and recombinant vesicles

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