Membrane formation during sclerotization of Physarum polycephalum plasmodia

Stewart, Peter A.; Stewart, Babette Taylor

doi:10.1016/0014-4827(61)90006-4

Cited by 33 publications

(8 citation statements)

References 19 publications

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“…Although numerous ultrastructural studies of mature spherules have been carried out by light and electron microscopies, none of them determined the major polysaccharide of the spherule wall (Stewart and Stewart 1961, Rhea 1966, Kikuchi 1970, Goodman and Rusch 1970, Zaar and Kleinig 1975, Patterson and Scheetz 1977. Since around 1980, we have not found reports on spherulation, spherule-wall formation, and the major component of the cell wall of a spherule.…”

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confidence: 80%

Evidence for Deposition of Cellulose Prior to Dark-starvation Treatment During Spherulation in Physarum microplasmodia

et al. 2010

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Summary Physarum plasmodium living as a slimy mass of protoplast in the dark is fragmented into small multinucleated microplasmodia (mPL) in a liquid medium. When mPL were exposed to several unfavorable environments, they transformed into "spherules" with the cell wall structure. We established a synchronous spherule-induction system for mPL consisting of 3 steps:Step 1, subculture in the 1% medium (1% sucrose and 1% soytone peptone) for 84 h at 25°C; Step 2, preculture in the 2% medium (2% sucrose and 2% soytone peptone) for 72 h at 25°C; and Step 3, dark-starvation treatment in the spherulation medium for 96 h at 25°C. Approximately 100% spherulation was observed within 48 h in Step 3. By fluorescence microscopy, we confirmed for the first time that cellulose was the major component of the cell wall in the Physarum spherule. From the results of experiments using the synchronous spherule-induction system and those of experiments on the inhibitory effect of 2,6-dichlorobenzonitrile (DCB) on cellulose synthesis in mPL in the preculture period, we presumed that the nourished medium in the preculture period was essential for mPL prior to spherulation to attain 100% of spherule. Our in vivo labeling experiments revealed that mPL of multinucleated protoplasts produced cellulose in the preculture period by de novo synthesis. We conclude that the Physarum plasmodia always supply themselves with materials to adapt to unfavorable environmental conditions such as starvation as rapidly as they can, even when they are under excellent conditions.

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confidence: 80%

Evidence for Deposition of Cellulose Prior to Dark-starvation Treatment During Spherulation in Physarum microplasmodia

et al. 2010

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“…(Physaraceae) and concluded that the granules are composed of a phosphate compound. Mitochondrial granules have been observed and reported in other Physarales by Stewart and Stewart (1961) and Schuster (1964Schuster ( , 1965 but their function was not disscussed.…”

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confidence: 88%

Calcium Deposition in the Myxomycete Didymium squamulosum

Gustafson

Thurston

1974

Mycologia

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“…Fusion of vesicles as a means of cleavage and membrane formation has been suggested for other fungi (Blondel and Turian, 1960;Stewart and' Stewart, 1961;Moore, 1964;Schuster, 1964;Bracker and Williams, 1966). Blondel and Turian observed double rows of vesicles much smaller than the one reported here situated between two segments of already existing membranes.…”

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confidence: 96%

Ultrastructural Changes During Zoospore Formation in Phytophthora Parasitica

Hohl

Hamamoto

1967

American J of Botany

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Cleavage of the multinucleate sporangial cytoplasm begins by a rearrangement and subsequent fusion of randomly dispersed cleavage vesicles. The vesicles line up in planes equidistant from neighboring nuclei and along the sporangial wall. In addition, they contribute to the enlargement of the central vacuole. Fusion of these vesicles with themselves and with the central vacuole cleaves the cytoplasm into uninucleate zoospores, each with two flagella. The sporangial wall consists of two layers, an outer thin one which is continuous over the plug of the discharge pore and an inner thick one which tapers off near the plug. The plug consists of fibrillar material and is ejected upon release of the zoospores. A plug‐like structure separating the forming sporangium from the hypha has a homogeneous matrix pervaded with an anastomosing network of fine electron‐dense channels. In addition, glycogen‐like granules occur within mitochondria and paired structures are interpreted as procentrioles.

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Membrane formation during sclerotization of Physarum polycephalum plasmodia

Cited by 33 publications

References 19 publications

Evidence for Deposition of Cellulose Prior to Dark-starvation Treatment During Spherulation in Physarum microplasmodia

Evidence for Deposition of Cellulose Prior to Dark-starvation Treatment During Spherulation in Physarum microplasmodia

Calcium Deposition in the Myxomycete Didymium squamulosum

Ultrastructural Changes During Zoospore Formation in Phytophthora Parasitica

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