Membrane damage by lipid peroxidation retains the cadmium constraint and is not the primary cause of K+ extrusion in yeast

Costa-Moreira, Luciana M.; Porto, Bárbara Alves; Haddad-Ribeiro, Frederico; Martins, Flaviano S.; Menezes, M. A. B. C.; Rosa, Carlos A.; Neves, Maria J.

doi:10.1007/s13213-015-1181-3

Cited by 6 publications

(5 citation statements)

References 42 publications

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“…Although both metals have a negative effect on the cells, including yeasts, cadmium has a higher level of toxicity, even compared to other heavy metals such as mercury, arsenic or cobalt [ 38 ]. Cadmium ions determine cell membrane disruption and the efflux of K + by disturbing the copper and zinc uptake, which impairs the redox balance and mitochondrial respiration and generates free radicals and high levels of lipid peroxidation [ 39 , 40 ]. Moreover, Cd 2+ also affects vitamin metabolism and causes protein misfolding [ 41 ].…”

Section: Resultsmentioning

confidence: 99%

Bioremediation of Wastewater Using Yeast Strains: An Assessment of Contaminant Removal Efficiency

Nicula

Lungulescu

Rimbu

et al. 2023

IJERPH

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The main goal of wastewater treatment is to significantly reduce organic compounds, micronutrients (nitrogen and phosphorus) and heavy metals and other contaminants (pathogens, pharmaceuticals and industrial chemicals). In this work, the efficiency of removing different contaminants (COD, NO3−, NO2−, NH4+, PO43−, SO42−, Pb2+, Cd2+) from synthetic wastewater was tested using five different yeast strains: Kluyveromyces marxianus CMGBP16 (P1), Saccharomyces cerevisiae S228C (P2), Saccharomyces cerevisiae CM6B70 (P3), Saccharomyces cerevisiae CMGB234 (P4) and Pichia anomala CMGB88 (P5). The results showed a removal efficiency of up to 70% of COD, 97% of nitrate, 80% of nitrite, 93% of phosphate and 70% of sulfate ions for synthetic wastewater contaminated with Pb2+ (43 mg/L) and Cd2+ ions (39 mg/L). In contrast, the results showed an increase in ammonium ions, especially in the presence of Pb2+ ions. The yeast strains showed a high capacity to reduce Pb2+ (up to 96%) and Cd2+ (up to 40%) ions compared to the initial concentrations. In presence of a crude biosurfactant, the removal efficiency increased up to 99% for Pb2+ and 56% for Cd2+ simultaneously with an increase in yeast biomass of up to 11 times. The results, which were obtained in the absence of aeration and in neutral pH conditions, proved a high potential for practical applications in the biotreatment of the wastewater and the recovery of Pb and Cd ions, with a high benefit–cost ratio.

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Section: Resultsmentioning

confidence: 99%

Bioremediation of Wastewater Using Yeast Strains: An Assessment of Contaminant Removal Efficiency

Nicula

Lungulescu

Rimbu

et al. 2023

IJERPH

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“…Lipid peroxidation is one of the reactions triggered by free radical formation in the cell membrane. 48 The oxidative stress tolerance of S. cerevisiae acquired by resveratrol and catechin was correlated with a reduction in lipid peroxidation. 42 Nevertheless, consistent with our study, a previous study using in vitro assays demonstrated the antioxidative effects of highmolecular-weight persimmon tannins against hydroxylradical-scavenging activities using 2-deoxyribose oxidation and salicylic acid, superoxide anion scavenging activity, and inhibitory activity against lipid peroxidation using linoleic acid, suggesting high-molecular condensed tannins as the major antioxidants in persimmon.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, the significance of persimmon tannins with high molecular weight in protecting cells from oxidative damage, particularly lipid peroxidation, warrants further investigation. Lipid peroxidation is one of the reactions triggered by free radical formation in the cell membrane 48 . The oxidative stress tolerance of S. cerevisiae acquired by resveratrol and catechin was correlated with a reduction in lipid peroxidation 42 .…”

Section: Discussionmentioning

confidence: 99%

Persimmon tannin promotes the growth of Saccharomyces cerevisiae under ethanol stress

Ilhamzah,

Tsukuda,

Yamaguchi

et al. 2024

J Sci Food Agric

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BackgroundSaccharomyces cerevisiae plays a pivotal role in various industrial processes, including bioethanol production and alcoholic beverage fermentation. However, during these fermentations, yeasts are subjected to various environmental stresses, such as ethanol stress, which hinder cell growth and ethanol production. Genetic manipulations and the addition of natural ingredients rich in antioxidants to the culture has been shown to overcome this. Here, we investigated the potential of persimmon tannins, known for their antioxidative properties, to enhance the ethanol stress tolerance of yeast.ResultsThe assessment of effects of 6.25 mg mL−1 persimmon tannins after 48 h of incubation revealed cell viability to be increased by 8.9‐ and 6.5‐fold compared to the control treatment with and without 12.5% ethanol, respectively. Furthermore, persimmon tannins reduced ethanol‐induced oxidative stress, including the production of cellular reactive oxygen species and acceleration of lipid peroxidation. However, persimmon tannins could hardly overcome ethanol‐induced cell membrane damage.ConclusionThe findings herein indicate the potential of persimmon tannin as a protective agent for increasing yeast tolerance to ethanol stress by restricting oxidative damage, but not membrane damage. Overall, this study unveils the implications of persimmon tannins for industries relying on yeast.This article is protected by copyright. All rights reserved.

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“…We measured the total free SH content to quantify GSH (Costa-Moreira et al, 2016;Porto et al, 2016) since a decrease in the free SH residue concentration corresponds to a diminution in GSH concentration, and consequently, attenuated antioxidant capacity. The oxidation of GSH maintains the redox balance when there are oxidants in the medium.…”

Section: Discussionmentioning

confidence: 99%

Radiolytic Synthesis and Characterization of Selenium Nanoparticles: Comparative Biosafety Evaluation with Selenite and Ionizing Radiation

Pereira

Gerolis

Gonçalves

et al. 2021

Preprint

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Selenium nanoparticles (SeNPs) were synthesized by irradiating a solution containing sodium selenite (Se+4) as the precursor in YPG liquid medium with gamma-rays. Spherical particles were formed after reactions of sodium selenite with hydrated electrons (eaq-) and hydrogen radicals (H•) produced following water radiolysis. No hazardous reducing agents were employed. The obtained nanoparticles were morphologically characterized, and their physicochemical and structural parameters were analyzed. SeNPs characterization showed all selenium in the Se0 state. We incubated Saccharomyces cerevisiae cells with the SeNPs for 24 h and then challenged the cells with ionizing radiation. After radiation exposure, cells were assessed for cell viability, lipid peroxidation, protein carbonylation, free radical generation, and total sulfhydryl content. The synthesized SeNPs were considered safe and less toxic at the concentration employed than the same selenite concentration. Except for the protein carbonylation results, there were no other significant modifications in viability or the oxidative stress parameters in SeNP-treated cells. It was concluded that 1 mM of the synthesized SeNPs does not trigger oxidative stress. Furthermore, we verified that SeNPs attenuate the reactive oxygen species generation after in vitro ionizing radiation exposure. These observations open up tremendous possibilities for radiosensitizer development.

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Membrane damage by lipid peroxidation retains the cadmium constraint and is not the primary cause of K+ extrusion in yeast

Cited by 6 publications

References 42 publications

Bioremediation of Wastewater Using Yeast Strains: An Assessment of Contaminant Removal Efficiency

Bioremediation of Wastewater Using Yeast Strains: An Assessment of Contaminant Removal Efficiency

Persimmon tannin promotes the growth of Saccharomyces cerevisiae under ethanol stress

Radiolytic Synthesis and Characterization of Selenium Nanoparticles: Comparative Biosafety Evaluation with Selenite and Ionizing Radiation

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