Membrane curvature based lipid sorting using a nanoparticle patterned substrate

Black, Joshua C.; Cheney, Philip P.; Campbell, Travis; Knowles, Michelle K.

doi:10.1039/c3sm52522h

Cited by 38 publications

(63 citation statements)

References 46 publications

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“…Although the Pearson’s correlation coefficients are quite small, DHPE is significantly higher than HDA, indicating that the lipids are weakly associated with curvature, but DHPE associates more. It is interesting to note that in our bilayer system, the bilayer composition is symmetric and higher degrees of sorting have been observed when we added lipids specifically to the upper leaflet only [18]. …”

Section: Resultsmentioning

confidence: 99%

“…This suggests that molecules avoid curvature. It is also difficult to find trajectories that transition from flat to curved regions, yet FRAP data has shown that lipids’ NP positions recover [18] and the single molecule tracking of SB-DHPE clearly demonstrates that movement of molecules at sites of curvature is very confined (Figure 5). …”

Section: Discussionmentioning

confidence: 99%

“…Membrane shape has recently been identified as an instigator and stabilizer of lipid domain formation [18,19,20,21,22,23,24], but the mechanism by which lipids sort into curved regions is not clear. Curvature based lipid sorting has been observed in experimental work where curved tubular membranes are created from giant unilamellar vesicles [22,25] and in curved bilayers [26] for lipid mixtures.…”

Section: Introductionmentioning

confidence: 99%

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Single Lipid Molecule Dynamics on Supported Lipid Bilayers with Membrane Curvature

et al. 2017

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The plasma membrane is a highly compartmentalized, dynamic material and this organization is essential for a wide variety of cellular processes. Nanoscale domains allow proteins to organize for cell signaling, endo- and exocytosis, and other essential processes. Even in the absence of proteins, lipids have the ability to organize into domains as a result of a variety of chemical and physical interactions. One feature of membranes that affects lipid domain formation is membrane curvature. To directly test the role of curvature in lipid sorting, we measured the accumulation of two similar lipids, 1,2-Dihexadecanoyl-sn-glycero-3-phosphoethanolamine (DHPE) and hexadecanoic acid (HDA), using a supported lipid bilayer that was assembled over a nanopatterned surface to obtain regions of membrane curvature. Both lipids studied contain 16 carbon, saturated tails and a head group tag for fluorescence microscopy measurements. The accumulation of lipids at curvatures ranging from 28 nm to 55 nm radii was measured and fluorescein labeled DHPE accumulated more than fluorescein labeled HDA at regions of membrane curvature. We then tested whether single biotinylated DHPE molecules sense curvature using single particle tracking methods. Similar to groups of fluorescein labeled DHPE accumulating at curvature, the dynamics of single molecules of biotinylated DHPE was also affected by membrane curvature and highly confined motion was observed.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Single Lipid Molecule Dynamics on Supported Lipid Bilayers with Membrane Curvature

et al. 2017

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“…27,62 Furthermore, the NP size can modulate the corona formation due to curvature-related changes in the membrane composition 63–68 and NP-protein interactions. 26,69 The National Institutes of Health defines nanomaterials as materials with characteristic length scales < 100 nm.…”

Section: Resultsmentioning

confidence: 99%

Lipid-Mediated Targeting with Membrane-Wrapped Nanoparticles in the Presence of Corona Formation

Reiser²,

et al. 2016

ACS Nano

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Membrane wrapped nanoparticles represent a versatile platform for utilizing specific lipid-receptor interactions, such as siallyllactose-mediated binding of the ganglioside GM3 to Siglec1 (CD169), for targeting purposes. The membrane wrap around the nanoparticles does not only serve as a matrix to incorporate GM3 as targeting moiety for antigen presenting cells but also offers unique opportunities for constructing a biomimetic surface from lipids with potentially protein repellent properties. We characterize non-specific protein adsorption (corona formation) to membrane wrapped nanoparticles with core diameters of approx. 35 nm and 80 nm and its effect on the GM3-mediated targeting efficacy as function of surface charge through combined in vitro and in vivo studies. The stability and fate of the membrane wrap around the nanoparticles in a simulated biological fluid and after uptake in CD169 expressing antigen presenting cells is experimentally tested. Finally, we demonstrate in hock immunization studies in mice that GM3 decorated membrane wrapped nanoparticles achieve a selective enrichment in the peripheral regions of popliteal lymph nodes that contain high concentrations of CD169 expressing antigen presenting cells.

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“…These methods were mostly based on fluorescence and included Förster Resonance Energy Transfer (FRET) 25 , Fluorescence Correlation Spectroscopy (FCS) 26 , Fluorescence Recovery after Photobleaching (FRAP) 27,28 , Fluorescence Anisotropy 29 and Generalized Polarization (GP) of the Laurdan probe 30 . These studies have included investigations of lipid behavior in nanoparticle-modified lipid bilayer systems 28,31,32 . Some of these techniques can – in principle – be implemented on the single-cell or even -virus level.…”

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confidence: 99%

Membrane Fluidity Sensing on the Single Virus Particle Level with Plasmonic Nanoparticle Transducers

Feizpour¹,

Stelter²,

Wong³

et al. 2017

ACS Sens.

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Viral membranes are nanomaterials whose fluidity depends on their composition, in particular the cholesterol (chol) content. As differences in the membrane composition of individual virus particles can lead to different intracellular fates, biophysical tools capable of sensing the membrane fluidity on the single-virus level are required. In this manuscript, we demonstrate that fluctuations in the polarization of light scattered off gold or silver nanoparticle (NP)-labeled virus-like-particles (VLPs) encode information about the membrane fluidity of individual VLPs. We developed plasmonic polarization fluctuation tracking microscopy (PFTM) which facilitated the investigation of the effect of chol content on the membrane fluidity and its dependence on temperature, for the first time on the single-VLP level. Chol extraction studies with different methyl-β-cyclodextrin (MβCD) concentrations yielded a gradual decrease in polarization fluctuations as function of time. The rate of chol extraction for individual VLPs showed a broad spread, presumably due to differences in the membrane composition for the individual VLPs, and this heterogeneity increased with decreasing MβCD concentration.

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Membrane curvature based lipid sorting using a nanoparticle patterned substrate

Cited by 38 publications

References 46 publications

Single Lipid Molecule Dynamics on Supported Lipid Bilayers with Membrane Curvature

Single Lipid Molecule Dynamics on Supported Lipid Bilayers with Membrane Curvature

Lipid-Mediated Targeting with Membrane-Wrapped Nanoparticles in the Presence of Corona Formation

Membrane Fluidity Sensing on the Single Virus Particle Level with Plasmonic Nanoparticle Transducers

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