Membrane curvature at a glance

McMahon, Harvey T.; Boucrot, Emmanuel

doi:10.1242/jcs.114454

Cited by 636 publications

(616 citation statements)

References 84 publications

(100 reference statements)

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“…Cell organelles have specific shapes depending on their functions. Various types of proteins participate in the regulation of these dynamic and static membrane shapes [1][2][3][4][5][6]. These proteins mainly control local membrane shapes in two ways: hydrophobic insertions (wedging) and scaffolding.…”

Section: Introductionmentioning

confidence: 99%

Membrane structure formation induced by two types of banana-shaped proteins

Noguchi

Fournier

2017

Soft Matter

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The assembly of banana-shaped rodlike proteins on membranes, and the associated membrane shape transformations, are investigated by analytical theory and coarse-grained simulations. The membrane-mediated interactions between two banana-shaped inclusions are derived theoretically using a point-like formalism based on fixed anisotropic curvatures, both for zero surface tension and for finite surface tension. On a larger scale, the interactions between assemblies of such rodlike inclusions are determined analytically. Meshless membrane simulations are performed in the presence of a large number of inclusions of two types, corresponding to curved rods of opposite curvatures, both for flat membranes and vesicles. Rods of the same type aggregate into linear assemblies perpendicular to the rod axis, leading to membrane tubulation. However, rods of the other type, those of opposite curvature, are attracted to the lateral sides of these assemblies, and stabilize a straight bump structure that prevents tubulation. When the two types of rods have almost opposite curvatures, the bumps attract one another, forming a stripe structure. Positive surface tension is found to stabilize the stripe formation. The simulation results agree well with the theoretical predictions provided the point-like curvatures of the model are scaled-down to account for the effective flexibility of the simulated rods.

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Section: Introductionmentioning

confidence: 99%

Membrane structure formation induced by two types of banana-shaped proteins

Noguchi

Fournier

2017

Soft Matter

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“…Cell organelles have specific shapes depending on their functions. Various types of proteins participate in regulation of these dynamic and static membrane shapes [1][2][3][4][5][6]. These proteins mainly control local membrane shapes in two ways: hydrophobic insertions (wedging) and scaffolding.…”

Section: Introductionmentioning

confidence: 99%

Shape deformation of lipid membranes by banana-shaped protein rods: Comparison with isotropic inclusions and membrane rupture

Noguchi

2016

Phys. Rev. E

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The assembly of curved protein rods on fluid membranes is studied using implicit-solvent meshless membrane simulations. As the rod curvature increases, the rods on a membrane tube assemble along the azimuthal direction first and subsequently along the longitudinal direction. Here, we show that both transition curvatures decrease with increasing rod stiffness. For comparison, curvature-inducing isotropic inclusions are also simulated. When the isotropic inclusions have the same bending rigidity as the other membrane regions, the inclusions are uniformly distributed on the membrane tubes and vesicles even for large spontaneous curvature of the inclusions. However, the isotropic inclusions with much larger bending rigidity induce shape deformation and are concentrated on the region of a preferred curvature. For high rod density, high rod stiffness, and/or low line tension of the membrane edge, the rod assembly induces vesicle rupture, resulting in the formation of a high-genus vesicle. A gradual change in the curvature suppresses this rupture. Hence, large stress, compared to the edge tension, induced by the rod assembly is the key factor determining rupture. For rod curvature with the opposite sign to the vesicle curvature, membrane rupture induces inversion of the membrane, leading to division into multiple vesicles as well as formation of a high-genus vesicle.

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“…Members of the Bin/Amphiphysin/Rvs167 (BAR) family of proteins dimerize to form a crescent-shaped membrane-interacting scaffold that can stabilize or generate membrane curvature (1). It remains poorly understood how these scaffolds are targeted to specific membranes in vivo, and how their activities are regulated to direct membrane dynamics in diverse contexts.…”

mentioning

confidence: 99%

Coordinated autoinhibition of F-BAR domain membrane binding and WASp activation by Nervous Wreck

Stanishneva‐Konovalova

Kelley

Eskin

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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Membrane remodeling by Fes/Cip4 homology-Bin/Amphiphysin/Rvs167 (F-BAR) proteins is regulated by autoinhibitory interactions between their SRC homology 3 (SH3) and F-BAR domains. The structural basis of autoregulation, and whether it affects interactions of SH3 domains with other cellular ligands, remain unclear. Here we used single-particle electron microscopy to determine the structure of the F-BAR protein Nervous Wreck (Nwk) in both soluble and membrane-bound states. On membrane binding, Nwk SH3 domains do not completely dissociate from the F-BAR dimer, but instead shift from its concave surface to positions on either side of the dimer. Unexpectedly, along with controlling membrane binding, these autoregulatory interactions inhibit the ability of Nwk-SH3a to activate Wiskott–Aldrich syndrome protein (WASp)/actin related protein (Arp) 2/3-dependent actin filament assembly. In Drosophila neurons, Nwk autoregulation restricts SH3a domain-dependent synaptopod formation, synaptic growth, and actin organization. Our results define structural rearrangements in Nwk that control F-BAR–membrane interactions as well as SH3 domain activities, and suggest that these two functions are tightly coordinated in vitro and in vivo.

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Membrane curvature at a glance

Cited by 636 publications

References 84 publications

Membrane structure formation induced by two types of banana-shaped proteins

Membrane structure formation induced by two types of banana-shaped proteins

Shape deformation of lipid membranes by banana-shaped protein rods: Comparison with isotropic inclusions and membrane rupture

Coordinated autoinhibition of F-BAR domain membrane binding and WASp activation by Nervous Wreck

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