Membrane crystallization of lysozyme under forced solution flow

Curcio, Efrem; Simone, Silvia De; Profio, Gianluca Di; Drioli, Enrico; Cassetta, Alberto; Lamba, Doriano

doi:10.1016/j.memsci.2004.07.037

Cited by 57 publications

(61 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The chemical and physical nature of the membrane surface influences the mechanisms of solute interaction/aggregation with important effects on the crystallization processes, so that accelerated crystallization kinetics with lower induction times than normal, 22,46-48 but with preserved diffracting crystal quality, are generally observed with MAC. [49][50][51] In Fig. 6.11 are reported SEM micrographs of typical porous membrane surfaces with tetragonal lysozyme and orthorhombic trypsin crystals embedded on the polymeric surface.…”

Section: Heterogeneous Nucleation By Porous Membranesmentioning

confidence: 99%

Crystallization of Biomacromolecules

Drioli¹,

Profio²,

Curcio³

2015

Membrane-Assisted Crystallization Technology

View full text Add to dashboard Cite

The crystallization of biomacromolecules by using membrane-assisted crystallization technology is described in this chapter. The discussion will focus on the interest in growing protein crystals and the current difficulties in obtaining them by conventional crystallization methods, the possibility to use MAC for the growth of protein crystals and the influence of controlling the transmembrane flux on crystallization kinetics, the function of polymeric membranes as solid support for heterogeneous nucleation and the correlation between membrane physical properties and the energetics of nucleation, and the effect of forced solution-flow convection on crystallization kinetics and its influence on crystal properties. Interest in Protein CrystallizationThe functions of biomolecules are strictly connected to their three-dimensional molecular structures. Therefore, the determination of the spatial arrangement of atoms or groups of atoms in protein molecules is of fundamental importance for the understanding of their biological activity and for designing new drug molecules that can act at active sites. 1 The 3D structure of proteins can be determined by X-ray or neutron diffraction and nuclear magnetic resonance (NMR) spectroscopy. However, the crystallographic method is the one of choice, especially for molecules that are too large (M.W. > 20 kDa). Crystallography gives a higher resolution, reaching less than 1 Å for particularly complex systems like membrane-protein or viruses. 2 The basic requisite for structure resolution at the atomic level by X-ray diffraction is the availability of well-diffracting crystals of adequate size. 3 193 Membrane-Assisted Crystallization Technology Downloaded from www.worldscientific.com by CHINESE UNIVERSITY OF HONG KONG on 10/09/15. For personal use only.194 Membrane-Assisted Crystallization TechnologyAmong proteins, enzymes are the most efficient known catalysts because of their high substrate specificity. 4 However, their systematic utilization in solution at industrial scale has been rather limited due to their intrinsic labile nature under operative conditions. Poor chemical and mechanical stability at extreme temperatures and pH, high pressure, mechanical stress, and the presence of denaturing solvents and other chemicals, are the factors that have prevented enzymes from being extensively used. Also, in the solid state, proteins are normally characterized by pronounced fragility or shattering under relatively mild conditions. On the other hand, the possibility of producing a large amount of protein crystal with reduced, uniform, and predictable size, slow dissolution kinetics, and high chemical and mechanical stability would have remarkable implications in many biotechnological applications, e.g. for sustained constant rate drug release, in environmental protection applications, in chemical synthesis, etc. 5 In this respect, cross-linked enzyme crystals (CLECs) 6,7 are interesting systems whose production is a relatively simple, fast, and less expensive technique and one that does ...

show abstract

Section: Heterogeneous Nucleation By Porous Membranesmentioning

confidence: 99%

Crystallization of Biomacromolecules

Drioli¹,

Profio²,

Curcio³

2015

Membrane-Assisted Crystallization Technology

View full text Add to dashboard Cite

show abstract

“…With MACr, one can better control and limit the maximum level of supersaturation due to its well-defined mass transfer through the membrane, resulting in a favorable crystal size, shape and purity [4,5], Simultaneously, the membrane promotes heterogeneous nucleation, which in turn reduces the induction time (defined as the time passed between reaching supersaturation and the formation of crystals) of the crystallization process [6].…”

Section: Introductionmentioning

confidence: 99%

Enhanced performance of a biomimetic membrane for Na2CO3 crystallization in the scenario of CO2 capture

Lin

Madsen

et al. 2016

Journal of Membrane Science

View full text Add to dashboard Cite

Highlights-Aquaporin based FO membrane was applied as membrane crystallizer to crystallize Na 2 CO 3 -High water flux and low reverse salt flux can be realized by using this biomimetic membrane -Na 2 CO 3 ·10H 2 O crystals with a purity of 99.94% can be achieved -Negligible membrane fouling and membrane blockage were observed *Highlights (for review) Graphical Abstract:Graphical Abstract (for review) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35

show abstract

“…Membrane distillation can be classified into different types based on the method used to create the pressure difference, such as direct contact membrane distillation (DCMD), air gap membrane distillation, vacuum membrane distillation (VMD) and sweeping gas membrane distillation [1][2][3][4][5][6][7][8][9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Hydrophobic polyethersulfone porous membranes for membrane distillation

Abdallah

El–Gendi

Khedr

et al. 2015

Front. Chem. Sci. Eng.

View full text Add to dashboard Cite

Membrane distillation (MD) is a thermal, vapor-driven transportation process through micro porous hydrophobic membranes that is increasingly being applied to seawater and brine desalination processes. Two types of hydrophobic microporous polyethersulfone flat sheet membranes, namely, annealed polyethersulfone and a polyethersulfone/tetraethoxysilane (PES/TEOS) blend were prepared by a phase inversion process. The membranes were characterized and their performances were investigated using the vacuum membrane distillation of an aqueous NaCl solution. The performances of the prepared membranes were also compared with two commercially available hydrophobic membranes, polytetrafluorethylene and polyvinylidene fluoride. The influence of operational parameters such as feed temperature (25-65°C), permeate vacuum pressure (200-800 mbar), feed flow rate (8-22 mL/s) and feed salt concentration (3000 to 35000 mg/L) on the MD permeation flux were investigated for the four membranes. The hydrophobic PES/TEOS membrane had the highest salt rejection (99.7%) and permeate flux (86 kg/(m 2 $h)) at 65°C, with a feed of 7000 ppm and a pressure of 200 mbar.

show abstract

Membrane crystallization of lysozyme under forced solution flow

Cited by 57 publications

References 35 publications

Crystallization of Biomacromolecules

Crystallization of Biomacromolecules

Enhanced performance of a biomimetic membrane for Na2CO3 crystallization in the scenario of CO2 capture

Hydrophobic polyethersulfone porous membranes for membrane distillation

Contact Info

Product

Resources

About