1971
DOI: 10.1021/bi00800a015
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Membrane-bound phospholipase A1 purified from Escherichia coli

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Cited by 214 publications
(107 citation statements)
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References 44 publications
(34 reference statements)
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“…On the other hand, about 30% of original lysophospholipase activity was lost by heat-treatment at 100° for 10 min. These properties of the phospholipase A of this organism are quite similar to those of dr-phospholipase A of E. coli with respect to resistance to detergent and heat, and Ca2+ requirement (15,16). Dr-phospholipase A of E. coli has been found to be located in the outer membrane (16)(17)(18).…”
Section: Phospholipasesupporting
confidence: 58%
“…On the other hand, about 30% of original lysophospholipase activity was lost by heat-treatment at 100° for 10 min. These properties of the phospholipase A of this organism are quite similar to those of dr-phospholipase A of E. coli with respect to resistance to detergent and heat, and Ca2+ requirement (15,16). Dr-phospholipase A of E. coli has been found to be located in the outer membrane (16)(17)(18).…”
Section: Phospholipasesupporting
confidence: 58%
“…The sole modification in procedure was elimination of 2-mercaptoethanol since this compound can reduce nitroxides. The separation of inner and outer membranes was monitored by assay of appropriate enzyme markers, succinate dehydrogenase for inner membranes (27) and phospholipase A for outer membranes (28). The inner membrane preparations used here contained a maximum of 15% outer membrane contamination.…”
Section: Methodsmentioning
confidence: 99%
“…One factor important in dormant spore resistance to such chemicals appears to be their slow permeation into the spore core or protoplast. A variety of data indicate that it is the spore's inner membrane that is the major permeability barrier restricting the passage of small molecules into the spore core (3-7), although the lipid composition of the inner membrane exhibits no anomalies that might explain its unusual properties (8)(9)(10)(11)(12). In addition, the dormant spore's inner membrane has the potential to expand significantly, because electron microscopy indicates that the volume this membrane encompasses (the spore core): (i) decreases as much as 2-fold late in sporulation (13); and (ii) increases up to 2-fold in the first minute of spore germination, when the spore's large peptidoglycan cortex is degraded and the germ cell wall expands (13)(14)(15).…”
mentioning
confidence: 99%