Membrane bound Indian clade C HIV-1 envelope antigen induces antibodies to diverse and conserved epitopes upon DNA prime/protein boost in rabbits

Rangasamy, Sneha Priya; Menon, Veena; Priyanka, Dhopeshwarkar; Pal, Ranajit; Vaniambadi, K; Mahalingam, Sundarasamy

doi:10.1016/j.vaccine.2016.03.062

Cited by 3 publications

(1 citation statement)

References 44 publications

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“…Codon-optimized genes of both SIVmac251 gp140 and SIVsmE660 gp145 were synthesized by abolishing the gp120-gp41 cleavage site and eliminating the transmembrane domain (TM) and cytoplasmic region of gp160, and then cloned into an expression vector under the control of the CMV promoter. Trimeric SIV envelope proteins were stably expressed following transfection of the Env expression vector DNAs into 293 cells and purified to homogeneity by lectin affinity, anion exchange and gel filtration chromatography [31]. As shown in Fig.…”

Section: Antigenic and Immunogenic Characterization Of Trimeric Siv Ementioning

confidence: 99%

DNA prime/protein boost vaccination elicits robust humoral response in rhesus macaques using oligomeric simian immunodeficiency virus envelope and Advax delta inulin adjuvant

Mittal

Ayala

Rangaswamy

et al. 2017

Journal of General Virology

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The partial success of the RV144 trial underscores the importance of envelope-specific antibody responses for an effective HIV-1 vaccine. Oligomeric HIV-1 envelope proteins delivered with a potent adjuvant are expected to elicit strong antibody responses with broad neutralization specificity. To test this hypothesis, two SIV envelope proteins were formulated with delta inulin-based adjuvant (Advax) and used to immunize nonhuman primates. Oligomeric gp140-gp145 from SIVmac251 and SIVsmE660 was purified to homogeneity. Oligomers showed high-affinity interaction with CD4 and were highly immunogenic in rabbits, inducing Tier 2 SIV-neutralizing antibodies. The immunogenicity of an oligomeric Env DNA prime and protein boost together with Advax was evaluated in Chinese rhesus macaques. DNA administration elicited antibodies to both envelopes, and titres were markedly enhanced following homologous protein boosts via intranasal and intramuscular routes. Strong antibody responses were detected against the V1 and V2 domains of gp120. During peak immune responses, a low to moderate level of neutralizing activity was detected against Tier 1A/1B SIV isolates, with a moderate level noted against a Tier 2 isolate. Increased serum antibody affinity to SIVmac251 gp140 and generation of Env-specific memory B cells were observed in the immunized macaques. Animals were subjected to low-dose intravaginal challenge with SIVmac251 one week after the last protein boost. One out of three immunized animals was protected from infection. Although performed with a small number of macaques, this study demonstrates the utility of oligomeric envelopes formulated with Advax in eliciting broad antibody responses with the potential to provide protection against SIV transmission.

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Section: Antigenic and Immunogenic Characterization Of Trimeric Siv Ementioning

confidence: 99%

DNA prime/protein boost vaccination elicits robust humoral response in rhesus macaques using oligomeric simian immunodeficiency virus envelope and Advax delta inulin adjuvant

Mittal

Ayala

Rangaswamy

et al. 2017

Journal of General Virology

Self Cite

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Analysis of sequence diversity and selection pressure in HIV-1 clade C gp41 from India

et al. 2020

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Focus on the therapeutic efficacy of 3BNC117 against HIV-1: In vitro studies, in vivo studies, clinical trials and challenges

Liu

Bai

Liu

et al. 2017

International Immunopharmacology

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Membrane bound Indian clade C HIV-1 envelope antigen induces antibodies to diverse and conserved epitopes upon DNA prime/protein boost in rabbits

Cited by 3 publications

References 44 publications

DNA prime/protein boost vaccination elicits robust humoral response in rhesus macaques using oligomeric simian immunodeficiency virus envelope and Advax delta inulin adjuvant

DNA prime/protein boost vaccination elicits robust humoral response in rhesus macaques using oligomeric simian immunodeficiency virus envelope and Advax delta inulin adjuvant

Analysis of sequence diversity and selection pressure in HIV-1 clade C gp41 from India

Focus on the therapeutic efficacy of 3BNC117 against HIV-1: In vitro studies, in vivo studies, clinical trials and challenges

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