Membrane-bound class III peroxidases: Identification, biochemical properties and sequence analysis of isoenzymes purified from maize (Zea mays L.) roots

Mika, Angela; Buck, Friedrich; Lüthje, Sabine

doi:10.1016/j.jprot.2008.06.006

Cited by 43 publications

(57 citation statements)

References 54 publications

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“…In contrast to the above results, it has been reported that plasma membranes isolated from maize roots do contain substantial amounts of tightly bound peroxidase (Mika and Lüthje 2003;Mika et al 2008Mika et al , 2010. To clarify this apparent discrepancy we determined the peroxidase activity in plasma membrane preparations obtained from various parts of soybean and maize seedlings.…”

Section: Resultsmentioning

confidence: 65%

“…Plant peroxidases (type III) are secretory enzymes normally localized in the cell wall (Mika et al 2004). However, there is also evidence for peroxidases tightly bound to the plasma membrane (Zancani et al 1995;Mika and Lüthje 2003;Mika et al 2008). In view of their function as Fenton catalysts in the generation of OHÁ, the localization of peroxidases to, or remote from, the plasma membrane is important.…”

Section: Introductionmentioning

confidence: 99%

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Oxygen activation at the plasma membrane: relation between superoxide and hydroxyl radical production by isolated membranes

Heyno

Mary

Schöpfer

et al. 2011

Planta

104

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Production of reactive oxygen species (hydroxyl radicals, superoxide radicals and hydrogen peroxide) was studied using EPR spin-trapping techniques and specific dyes in isolated plasma membranes from the growing and the non-growing zones of hypocotyls and roots of etiolated soybean seedlings as well as coleoptiles and roots of etiolated maize seedlings. NAD(P)H mediated the production of superoxide in all plasma membrane samples. Hydroxyl radicals were only produced by the membranes of the hypocotyl growing zone when a Fenton catalyst (FeEDTA) was present. By contrast, in membranes from other parts of the seedlings a low rate of spontaneous hydroxyl radical formation was observed due to the presence of small amounts of tightly bound peroxidase. It is concluded that apoplastic hydroxyl radical generation depends fully, or for the most part, on peroxidase localized in the cell wall. In soybean plasma membranes from the growing zone of the hypocotyl pharmacological tests showed that the superoxide production could potentially be attributed to the action of at least two enzymes, an NADPH oxidase and, in the presence of menadione, a quinone reductase.

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Section: Resultsmentioning

confidence: 65%

Section: Introductionmentioning

confidence: 99%

Oxygen activation at the plasma membrane: relation between superoxide and hydroxyl radical production by isolated membranes

Heyno

Mary

Schöpfer

et al. 2011

Planta

104

View full text Add to dashboard Cite

show abstract

“…quinone reductases; Serrano et al, 1994;Trost et al, 1997;Schopfer et al, 2008;and MDA reductase;Bérczi and Møller, 1998), b-type cytochromes Bérczi et al, 2003;Preger et al, 2005), and peroxidases (Mika et al, 2008). Moreover, molecular biology approaches led to the discovery of flavocytochromes of the FRO family that use cytosolic pyridine nucleotides to reduce apoplastic ferrichelates (Robinson et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

Auxin-Responsive Genes AIR12 Code for a New Family of Plasma Membrane b-Type Cytochromes Specific to Flowering Plants

et al. 2009

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We report here on the identification of the major plasma membrane (PM) ascorbate-reducible b-type cytochrome of bean (Phaseolus vulgaris) and soybean (Glycine max) hypocotyls as orthologs of Arabidopsis (Arabidopsis thaliana) AIR12 (for auxin induced in root cultures). Soybean AIR12, which is glycosylated and glycosylphosphatidylinositol-anchored to the external side of the PM in vivo, was expressed in Pichia pastoris in a recombinant form, lacking the glycosylphosphatidylinositol modification signal and purified from the culture medium. Recombinant AIR12 is a soluble protein predicted to fold into a b-sandwich domain and belonging to the DOMON (for dopamine b-monooxygenase N terminus) domain superfamily. It is shown to be a b-type cytochrome with a symmetrical a-band at 561 nm, fully reduced by ascorbate, and fully oxidized by monodehydroascorbate radical. AIR12 is a high-potential cytochrome b showing a wide bimodal dependence from the redox potential between +80 mV and +300 mV. Optical absorption and electron paramagnetic resonance analysis indicate that AIR12 binds a single, highly axial low-spin heme, likely coordinated by methionine-91 and histidine-76, which are strongly conserved in AIR12 sequences. Phylogenetic analyses reveal that the auxin-responsive genes AIR12 represent a new family of PM b-type cytochromes specific to flowering plants. Circumstantial evidence suggests that AIR12 may interact with other redox partners within the PM to constitute a redox link between cytoplasm and apoplast.

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“…The amount of sample applied to each well was quantified according to the ability to oxidize 0.2 µmol ferulic acid/min. After separation, isoforms of POD were stained in 250 mM sodium acetate buffer, pH 5.0 containing 1% guaiacol and 0.03% H 2 O 2 (Mika et al, 2008).…”

Section: Isoelectric Focusing Of Peroxidase Isoformsmentioning

confidence: 99%

Modification of antioxidant systems in cell walls of maize roots by different nitrogen sources

Šukalović¹,

Vuletić

Marković

et al. 2017

Span. j. agric. res.

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Antioxidant systems of maize root cell walls grown on different nitrogen sources were evaluated. Plants were grown on a medium containing only NO 3 -or the mixture of NO 3 -+NH 4 + , in a 2:1 ratio. Eleven-day old plants, two days after the initiation of lateral roots, were used for the experiments. Cell walls were isolated from lateral roots and primary root segments, 2-7 cm from tip to base, representing zones of intense or decreased growth rates, respectively. Protein content and the activity of enzymes peroxidase, malate dehydrogenase and ascorbate oxidase ionically or covalently bound to the walls, as well as cell wall phenolic content and antioxidant capacity, were determined. Cell walls of plants grown on mixed N possess more developed enzymatic antioxidant systems and lower non-enzymatic antioxidant defenses than cell walls grown on NO 3 -. Irrespective of N treatment, the activities of all studied enzymes and protein content were higher in cell walls of lateral compared to primary roots. Phenolic content of cell walls isolated from lateral roots was higher in NO 3 --grown than in mixed N grown plants. No significant differences could be observed in the isozyme patterns of cell wall peroxidases isolated from plants grown on different nutrient solution. Our results indicate that different N treatments modify the antioxidant systems of root cell walls. Treatment with NO 3 -resulted in an increase of constitutive phenolic content, while the combination of NO 3 -+NH 4 + elevated the redox enzyme activities in root cell walls. Additional key words: nitrate; ammonia; antioxidant enzymes; phenolic compounds.

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Membrane-bound class III peroxidases: Identification, biochemical properties and sequence analysis of isoenzymes purified from maize (Zea mays L.) roots

Cited by 43 publications

References 54 publications

Oxygen activation at the plasma membrane: relation between superoxide and hydroxyl radical production by isolated membranes

Oxygen activation at the plasma membrane: relation between superoxide and hydroxyl radical production by isolated membranes

Auxin-Responsive Genes AIR12 Code for a New Family of Plasma Membrane b-Type Cytochromes Specific to Flowering Plants

Modification of antioxidant systems in cell walls of maize roots by different nitrogen sources

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