Membrane-based lateral flow immunochromatographic strip with nanoparticles as reporters for detection: A review

Huang, Xiaolin; Aguilar, Zoraida P.; Xu, Hengyi; Lai, Weihua; Xiong, Yonghua

doi:10.1016/j.bios.2015.08.032

Cited by 420 publications

(221 citation statements)

References 134 publications

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“…The principle of LFIA is based on antibody-antigen specific interaction. After the sample is applied to the sample pad, it migrates along the test strip via capillary action, and a signal response is obtained about 5-10 min later [39,40]. Due to its simplicity, rapidity, low cost, portability, and facile interpretation without external reagent or external instrumentation, LFIA has held great potential for foodborne pathogen detection [15,16,41].…”

Section: Lateral Flow Immunoassay (Lfia)mentioning

confidence: 99%

“…Due to its simplicity, rapidity, low cost, portability, and facile interpretation without external reagent or external instrumentation, LFIA has held great potential for foodborne pathogen detection [15,16,41]. In addition, the LFIA can realize visual detection and quantitative detection by employing different labels, such as colloid gold, fluorescent materials, and magnetic beads [40,42,43]. Descriptions on some of the labels that are applied for pathogenic bacteria detection are presented in the following sections.…”

Section: Lateral Flow Immunoassay (Lfia)mentioning

confidence: 99%

“…Magnetic beads are another type of label, which can realize quantitative detection of targets by measuring the magnetic signal [40,57,59]. Due to the fact that they are strongly colored and can enrich and separate targets from complex matrix, magnetic beads are new attractive materials to construct a lateral flow assay, which will probably replace traditional labels.…”

Section: Magnetic Beads As Labelmentioning

confidence: 99%

See 2 more Smart Citations

Current and Emerging Technologies for Rapid Detection of Pathogens

Zeng¹,

Wang²,

Hu³

2018

Biosensing Technologies for the Detection of Pathogens - A Prospective Way for Rapid Analysis

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Foodborne diseases, caused by pathogenic bacteria, have become an important social issue in the field of food safety. It presents a widespread and growing threat to human health in both developed and developing countries. As such, techniques for the detection of foodborne pathogens and waterborne pathogens are urgently needed to prevent the occurrence of human foodborne infections. Although traditional culture-based bacterial isolation and identification are the "gold standard" methods with high preciseness, their drawbacks in time-consuming are inadequate for rapid detection of pathogen to reduce foodborne disease occurrence. Fortunately, with the development of biotechnologies and nanotechnologies, various kinds of new technologies for rapid detection of pathogens have been developed so far, such as nucleic acid-based methods, antibody-based methods, and aptamer-based assays. In this chapter, we summarized the principles and the application of some recent rapid detection technologies for pathogenic bacteria. Moreover, the advantages and disadvantages of the established and emerging rapid detection methods are addressed here.

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Section: Lateral Flow Immunoassay (Lfia)mentioning

confidence: 99%

Section: Lateral Flow Immunoassay (Lfia)mentioning

confidence: 99%

Section: Magnetic Beads As Labelmentioning

confidence: 99%

See 1 more Smart Citation

Current and Emerging Technologies for Rapid Detection of Pathogens

Zeng¹,

Wang²,

Hu³

2018

Biosensing Technologies for the Detection of Pathogens - A Prospective Way for Rapid Analysis

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“…In order to meet the requirement of different detection, diverse signal marker strips had been developed, including colloidal gold strip, magnetic nanoparticles strip, and fluorescence strip [9–12]. As the typical nanomaterials, gold nanoparticles (Au NPs) have the color-tunable property with size, which also can be functionalized with peptides to avoid degradation in order to facilitate to finish quick immunological reaction and display different degree of color for half-quantitative detection [1, 11, 13]. Because of their unique advantages, such as simplicity, rapidity, and ease of interpretation, Au NPs-based LFICS was widely used for rapid diagnosis [1, 11, 13].…”

Section: Introductionmentioning

confidence: 99%

Smartphone-Based Dual-Modality Imaging System for Quantitative Detection of Color or Fluorescent Lateral Flow Immunochromatographic Strips

et al. 2017

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Nowadays, lateral flow immunochromatographic assays are increasingly popular as a diagnostic tool for point-of-care (POC) test based on their simplicity, specificity, and sensitivity. Hence, quantitative detection and pluralistic popular application are urgently needed in medical examination. In this study, a smartphone-based dual-modality imaging system was developed for quantitative detection of color or fluorescent lateral flow test strips, which can be operated anywhere at any time. In this system, the white and ultra-violet (UV) light of optical device was designed, which was tunable with different strips, and the Sobel operator algorithm was used in the software, which could enhance the identification ability to recognize the test area from the background boundary information. Moreover, this technology based on extraction of the components from RGB format (red, green, and blue) of color strips or only red format of the fluorescent strips can obviously improve the high-signal intensity and sensitivity. Fifty samples were used to evaluate the accuracy of this system, and the ideal detection limit was calculated separately from detection of human chorionic gonadotropin (HCG) and carcinoembryonic antigen (CEA). The results indicated that smartphone-controlled dual-modality imaging system could provide various POC diagnoses, which becomes a potential technology for developing the next-generation of portable system in the near future.

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“…The lateral flow device (LFD) has been most popular because of its simplicity in design and its automatic function, each LFD unit requiring only a few drops of sample solution for operation. Comprehensive reviews [22][23][24] have described the lateral flow assay system in depth, covering a broad range of immunoassay procedures and including nucleic acid applications. Many successful ELISAs can be readily converted to LFDs using the same or similar immuno-reagents; important properties established for ELISAs such as the plots of concentration giving 50% inhibition (IC50) perform in a similar manner in LFDs.…”

Section: Lateral Flow Devices For Aflatoxinsmentioning

confidence: 99%

The Aflatoxin QuicktestTM—A Practical Tool for Ensuring Safety in Agricultural Produce

Sánchez‐Bayo¹,

Almeida²,

Williams³

et al. 2017

Poisoning - From Specific Toxic Agents to Novel Rapid and Simplified Techniques for Analysis

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Contamination of corn, peanuts, milk and dairy products with aflatoxins is a worldwide problem, particularly in subtropical regions where the climatic conditions are ideal for the growth of Aspergillus flavus, the fungi that produces these toxins. Developing countries have major difficulties in marketing these products abroad due to the stringent international regulations concerning this carcinogenic toxin. Adding to the problem is the analytical cost involved in monitoring the produce, which require sophisticated instrumentation and qualified personnel, neither of which are available for field testing. The development of a rapid Aflatoxin Quicktest™ provides an effective, reliable and cheaper option for screening levels of aflatoxin above the regulatory thresholds in such produce. The test consists of a lateral flow device (LFD) coated with antibodies specific to aflatoxin B1, although it detects other aflatoxins (i.e. G and M) with high cross-reactivity. Its high sensitivity allows analysis of these toxins in the range 2-40 μg/kg of sample in 15 minutes, plus the time for extraction, which varies among different products. Quantification of the test results is done using a Quick Reader, by comparing the readings of individual tests against a standard curve of the analytes in the same manner as it is done with any other analytical equipment. A validation study was carried out using peanuts from Australia and peanuts and corn from Timor-Leste to assess the performance of the Aflatoxin Quicktest™. Results obtained with the LFD showed a good correlation with the standard analytical measurements by HPLC-fluorescence (r 2 above 0.90 for all cases), indicating the Aflatoxin Quicktest™ is capable of measuring levels of aflatoxins accurately and reliably. Given their ease of use, low cost and fast processing time, the Aflatoxin Quicktest™ can be used for screening agricultural produce in countries that cannot afford the costly alternative of using specialised personnel and equipment.

show abstract

Membrane-based lateral flow immunochromatographic strip with nanoparticles as reporters for detection: A review

Cited by 420 publications

References 134 publications

Current and Emerging Technologies for Rapid Detection of Pathogens

Current and Emerging Technologies for Rapid Detection of Pathogens

Smartphone-Based Dual-Modality Imaging System for Quantitative Detection of Color or Fluorescent Lateral Flow Immunochromatographic Strips

The Aflatoxin QuicktestTM—A Practical Tool for Ensuring Safety in Agricultural Produce

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