Membrane Applications in Monoclonal Antibody Production

Davies, Jim; Smith, Martin

doi:10.1016/b978-1-85617-632-3.00006-9

Cited by 2 publications

(1 citation statement)

References 15 publications

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“…Precipitate was retained by a microfilter, allowing for a wash in DF mode. In CFF, turbulent flow along the membrane surface ensures better recovery from the filter (Davies and Smith, 2010), also reducing concentration polarization and fouling (van Reis and Zydney, 2007). A main advantage of precipitate recovery by CFF over centrifugation lies in avoiding the compaction of precipitate that occurs during centrifugation, which allows for shorter precipitate re-dissolution times using CFF (Hammerschmidt et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Integrated Process for Capture and Purification of Virus-Like Particles: Enhancing Process Performance by Cross-Flow Filtration

Hillebrandt

Vormittag

Bluthardt

et al. 2020

Front. Bioeng. Biotechnol.

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Virus-like particles (VLPs) are emerging nanoscale protein assemblies applied as prophylactic vaccines and in development as therapeutic vaccines or cargo delivery systems. Downstream processing (DSP) of VLPs comes both with challenges and opportunities, depending on the complexity and size of the structures. Filtration, precipitation/re-dissolution and size-exclusion chromatography (SEC) are potent technologies exploiting the size difference between product and impurities. In this study, we therefore investigated the integration of these technologies within a single unit operation, resulting in three different processes, one of which integrates all three technologies. VLPs, contained in clarified lysate from Escherichia coli, were precipitated by ammonium sulfate, washed, and re-dissolved in a commercial cross-flow filtration (CFF) unit. Processes were analyzed for yield, purity, as well as productivity and were found to be largely superior to a reference centrifugation process. Productivity was increased 2.6-fold by transfer of the wash and re-dissolution process to the CFF unit. Installation of a multimodal SEC column in the permeate line increased purity to 96% while maintaining a high productivity and high yield of 86%. In addition to these advantages, CFF-based capture and purification allows for scalable and disposable DSP. In summary, the developed setup resulted in high yields and purities, bearing the potential to be applied as an integrated process step for capture and purification of in vivo-assembled VLPs and other protein nanoparticles.

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Section: Introductionmentioning

confidence: 99%