Membrane action of DMSO and other chemical inducers of Friend leukaemic cell differentiation

Lyman, Gary H.; Preisler, Harvey D.; Papahadjopoulos, Demetrios

doi:10.1038/262360a0

Cited by 209 publications

(77 citation statements)

References 20 publications

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“…The cryoprotectant dimethyl sulfoxide is believed to stabilize membranes by decreasing their fluidity (23). Therefore, if membrane fluidity was a significant factor, then after treating A. albopictus cells with increasing concentrations of dimethyl sulfoxide we might expect to see correspondingly decreasing levels of cytotoxicity.…”

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Purification and properties of a 28-kilodalton hemolytic and mosquitocidal protein toxin of Bacillus thuringiensis subsp. darmstadiensis 73-E10-2

Drobniewski

1989

J Bacteriol

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The mosquitocidal crystal of Bacillus thuringiensis subsp. darmstadiensis 73-E10-2 was purified, bioassayed against third-instar Aedes aegypti larvae (50% lethal concentration, 7.5 ,ug/ml), and subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis, revealing polypeptides of 125, 50, 47, and 28 kilodaltons (kDa). When solubilized and proteolytically activated by insect gut proteases or proteinase K, the crystal was cytotoxic to insect and mammalian cells in vitro and was hemolytic. By using nondenaturing polyacrylamide gel electrophoresis, a polypeptide of 23 kDa, derived from the 28-kDa protoxin, was identified which was hemolytic and cytotoxic to Aedes albopictus, A. aegypti, and Choristoneurafumiferana CF1 insect cell lines. The 23-kDa polypeptide was purified by ion-exchange chromatography and gave 50% lethal dose values of 3.8, 3.3, and 6.9 ,ug/ml against A. albopictus, A. aegypti, and C. fumiferana CF1 cells lines, respectively. Cytotoxicity in vitro was both dose and temperature dependent, with a sigmoidal dose-response curve. The cytotoxicity of the 23-kDa toxin and the solubilized and proteolytically activated 8-endotoxin was inhibited by a range of phospholipids containing unsaturated fatty acids and by triglyceride and diglyceride dispersions. An interaction with membrane phospholipids appears important for toxicity. Polyclonal antisera prepared against the 23-kDa polypeptide did not cross-react with polypeptides in the native crystals of four other mosquitocidal strains.

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“…In this study, we purified a * Corresponding author. 23-kDa polypeptide from the &-endotoxin crystal of B. thuringiensis subsp. darmstadiensis 73-E10-2 and found it to be cytolytic and hemolytic in vitro.…”

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Purification and properties of a 28-kilodalton hemolytic and mosquitocidal protein toxin of Bacillus thuringiensis subsp. darmstadiensis 73-E10-2

Drobniewski

1989

J Bacteriol

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“…DMSO is one of the most commonly used cryoprotectants in cellular systems (1), where it colligatively reduces the melting point of aqueous solutions and exerts additional effects that prevent cellular damage on freezing/ vitrification. Additional effects not seen with conventional glycol-and saccharide-based cryoprotectants include an increase in lipid membrane permeability of an array of solutes (2), promotion of cell fusion (3) and differentiation (4), and enhanced membrane resealing after damage (5). These phenomena are well-documented along with their dependence on DMSO concentration, but the molecular behavior of DMSO near lipid membranes remains a mystery.…”

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Correlating steric hydration forces with water dynamics through surface force and diffusion NMR measurements in a lipid–DMSO–H ₂ O system

Schrader

Donaldson

Song

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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Dimethyl sulfoxide (DMSO) is a common solvent and biological additive possessing well-known utility in cellular cryoprotection and lipid membrane permeabilization, but the governing mechanisms at membrane interfaces remain poorly understood. Many studies have focused on DMSO-lipid interactions and the subsequent effects on membrane-phase behavior, but explanations often rely on qualitative notions of DMSO-induced dehydration of lipid head groups. In this work, surface forces measurements between gel-phase dipalmitoylphosphatidylcholine membranes in DMSO-water mixtures quantify the hydration-and solvationlength scales with angstrom resolution as a function of DMSO concentration from 0 mol% to 20 mol%. DMSO causes a drastic decrease in the range of the steric hydration repulsion, leading to an increase in adhesion at a much-reduced intermembrane distance. Pulsed field gradient NMR of the phosphatidylcholine (PC) head group analogs, dimethyl phosphate and tetramethylammonium ions, shows that the ion hydrodynamic radius decreases with increasing DMSO concentration up to 10 mol% DMSO. The complementary measurements indicate that, at concentrations below 10 mol%, the primary effect of DMSO is to decrease the solvated volume of the PC head group and that, from 10 mol% to 20 mol%, DMSO acts to gradually collapse head groups down onto the surface and suppress their thermal motion. This work shows a connection between surface forces, head group conformation and dynamics, and surface water diffusion, with important implications for soft matter and colloidal systems.dimethyl sulfoxide | hydration shell | membrane interactions | lipid solvation | hydrodynamic radius S olute additives (e.g., osmolytes or denaturants) often play a key role in modulating biological interactions, where molecules can recognize each other and form assemblies. Dimethyl sulfoxide [DMSO, (O = S)(CH 3 ) 2 ] and DMSO-water mixtures in particular have attracted particular interest in biology and chemistry. DMSO is one of the most commonly used cryoprotectants in cellular systems (1), where it colligatively reduces the melting point of aqueous solutions and exerts additional effects that prevent cellular damage on freezing/ vitrification. Additional effects not seen with conventional glycol-and saccharide-based cryoprotectants include an increase in lipid membrane permeability of an array of solutes (2), promotion of cell fusion (3) and differentiation (4), and enhanced membrane resealing after damage (5). These phenomena are well-documented along with their dependence on DMSO concentration, but the molecular behavior of DMSO near lipid membranes remains a mystery.Bulk properties of DMSO-water mixtures are well-characterized. The oxygen and sulfur atoms of DMSO have a partial negative and positive charge, respectively, giving rise to a dipole moment of 3.96 Debye that exceeds nearly all conventional solvents. Dielectric relaxation spectroscopy (6), molecular dynamics simulations (7), and 1 H NMR studies (8) have shown that bulk water forms hydrogen bonds...

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“…Friend erythroleukemic cells (FLC) undergo erythroid differentiation accompanied by the synthesis of heme and hemoglobin (Hb), the accumulation of globin messenger RNA (mRNA), and erythrocyte-specific membrane changes upon treatment with dimethyl sulfoxide (Me2SO) (1)(2)(3)(4)(5)(6) and other polar solvents (7,8). Although the mechanism of action is still unknown, evidence suggests that induction of differentiation by these agents may involve their interaction with the cell membrane (9)(10)(11)(12). However, stimulation of erythroid differentiation also occurs upon treatment of FLC with structurally unrelated compounds such as butyric acid (13), purine analogues (14), and hemin (15).…”

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Inhibition of transcription and translation of globin messenger RNA in dimethyl sulfoxide-stimulated Friend erythroleukemic cells treated with interferon.

Rossi¹,

Dolei²,

Cioé³

et al. 1977

Proc. Natl. Acad. Sci. U.S.A.

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The addition of appropriate doses of interferon (IF) to cultures of Friend erythroleukemic cells inhibits dimethyl sulfoxide (Me2SO)-stimulated erythroid differentiation. In this study, the synthesis of heme, hemoglobin, and globin mRNA in Me2SO-stimulated cultures, with or without IF added, was compared. Although the hemoglobin content in Me2SO+IF-treated cultures was reduced 6- to 9-fold compared to that of cultures treated with Me2SO alone, there was less than a 2-fold decrease in the amount of heme accumulated. Globin mRNA, although unchanged in size or base sequence, was reduced in content in the Me2SO+IF cultures. The level of reduction of globin mRNA was insufficient to account for the lack of globin synthesis. Thus, it appears that IF may operate on two levels--one involving the transcription of globin mRNA and the other involving its translation.

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Membrane action of DMSO and other chemical inducers of Friend leukaemic cell differentiation

Cited by 209 publications

References 20 publications

Purification and properties of a 28-kilodalton hemolytic and mosquitocidal protein toxin of Bacillus thuringiensis subsp. darmstadiensis 73-E10-2

Purification and properties of a 28-kilodalton hemolytic and mosquitocidal protein toxin of Bacillus thuringiensis subsp. darmstadiensis 73-E10-2

Correlating steric hydration forces with water dynamics through surface force and diffusion NMR measurements in a lipid–DMSO–H ₂ O system

Inhibition of transcription and translation of globin messenger RNA in dimethyl sulfoxide-stimulated Friend erythroleukemic cells treated with interferon.

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Membrane action of DMSO and other chemical inducers of Friend leukaemic cell differentiation

Cited by 209 publications

References 20 publications

Purification and properties of a 28-kilodalton hemolytic and mosquitocidal protein toxin of Bacillus thuringiensis subsp. darmstadiensis 73-E10-2

Purification and properties of a 28-kilodalton hemolytic and mosquitocidal protein toxin of Bacillus thuringiensis subsp. darmstadiensis 73-E10-2

Correlating steric hydration forces with water dynamics through surface force and diffusion NMR measurements in a lipid–DMSO–H 2 O system

Inhibition of transcription and translation of globin messenger RNA in dimethyl sulfoxide-stimulated Friend erythroleukemic cells treated with interferon.

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Correlating steric hydration forces with water dynamics through surface force and diffusion NMR measurements in a lipid–DMSO–H ₂ O system