Members of the GH3 Family of Proteins Conjugate 2,4-D and Dicamba with Aspartate and Glutamate

Chiu, Li-Wei; Heckert, Matthew J.; You, You; Albanese, Nicholas; Fenwick, Tamara; Siehl, Daniel L.; Castle, Linda A.; Tao, Yumin

doi:10.1093/pcp/pcy160

Cited by 13 publications

(24 citation statements)

References 52 publications

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“…capitata, phylogenetic clustering of Arabidopsis and BoGH3 proteins demonstrated that BoGH3 proteins can be divided into 3 groups (Group , , and ) ( Fig. 1A) [6,10,32,33]. It was found that Group consists of two Arabidopsis and four BoGH3 proteins, while Group consists of eight Arabidopsis and eleven BoGH3 proteins.…”

Section: Resultsmentioning

confidence: 97%

“…oleracea) in our study, twenty ve and twenty nine GH3 protein-coding genes were previously reported for cabbage type B. oleracea var. capitata in the comparison with B. napus genes by two independent studies, respectively [32,33].…”

Section: Resultsmentioning

confidence: 99%

“…Recently, twenty ve or twenty nine GH3 protein-coding gene models originating from cabbage-type B. oleracea var. capitata were identi ed from the investigation of genomic sequence of B. napus [32,33]. Out of twenty nine B. napus GH3 genes with B. oleracea var.…”

Section: Discussionmentioning

confidence: 99%

“…In rapeseed (Brassica napus) and its diploid ancestors, Chinese cabbage (Brassica rapa) and cabbage (Brassica oleracea var. capitata), up to sixty six GH3coding genes have been identi ed [32][33]. However, detailed study of GH3-coding genes in kale-type Brassica species (Brassica oleracea var.…”

Section: Introductionmentioning

confidence: 99%

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Genome-Wide Identification of GH3 Genes in Brassica Oleracea and Identification of a Promoter Region for Anther-Specific Expression of a GH3 Gene

Jeong

Park

et al. 2020

Preprint

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Background:The Gretchen Hagen 3 (GH3) genes encode acyl acid amido synthetases, many of which have been shown to modulate the amount of active plant hormones or their precursors. GH3 genes, especially Group Ⅲ subgroup 6 GH3 genes, and their expression patterns in economically important kale-type Brassica oleracea have not been systematically identified. Results:As a first step to understand regulation and molecular functions of Group Ⅲ subgroup 6 GH3 genes, thirty-four GH3 genes including four subgroup 6 genes were identified In B. oleracea var. oleracea, using TO1000. Synteny found around subgroup 6 GH3 genes in TO1000 and Arabidopsis indicated that these genes are evolutionarily related. Although expression of four subgroup 6 GH3 genes in TO1000 is not induced by auxin, gibberellic acid, and jasmonic acid, the genes show different organ-dependent expression patterns. Only one TO1000 subgroup 6 GH3 gene, Bo2g011210, is expressed in anthers when microspores, polarized microspores, and bicellular pollens are present, similar to two out of four syntenic Arabidopsis subgroup 6 GH3 genes. Detailed analyses of promoter activities of Bo2g011210 further showed that Bo2g011210 is expressed in tapetal cells and pollens in anther, and also expressed in leaf primordia and floral abscission zones. Conclusions:Sixty-two base pair (bp) region (-340 ~ -279 bp upstream from start codon) and about 450 bp region (-1489 to -1017 bp) in Bo2g011210 promoter were found to be important for expressions in anther and expressions in leaf primordia and floral abscission zones, respectively. The identified anther-specific promoter region will be useful to develop male sterile transgenic Brassica plants.

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Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genome-Wide Identification of GH3 Genes in Brassica Oleracea and Identification of a Promoter Region for Anther-Specific Expression of a GH3 Gene

Jeong

Park

et al. 2020

Preprint

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“…These results suggest that the decrease in 2,4-D may be due to molecules (i.e., putative proteins) secreted from anise cells and inactivated at high temperatures. In plants, amido synthase, GRETCHEN HAGEN 3 proteins and cytochrome P450 have proposed as the enzymes responsible for degradation of 2,4-D (Chiu et al 2018;Eyer et al 2016;Figueiredo et al 2018). Meanwhile, in anise cell cultures, glyoxisomes develop and higher activities of isocitrate lyase and malate synthase (glyoxysomal enzymes) are observed (Kudielka and Theimer 1983).…”

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confidence: 99%

Anise-cultured cells abolish 2,4-dichlorophenoxyacetic acid in culture medium

Tanida

Shiota

2019

Plant Biotechnology

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In anise (Pimpinella anisum, family Apiaceae), callus-like embryogenic cells (embryogenic callus) are induced by culturing hypocotyl explants in 2,4-dichlorophenoxyacetic acid (2,4-D)-containing medium, and somatic embryos are formed from embryogenic callus transferred into 2,4-D-free medium. Anise somatic embryos are also induced even if embryogenic callus is continually cultured in 2,4-D-containing medium without subculturing. In this study, we aimed to clarify the dynamics of 2,4-D during anise cell culture. After culturing anise callus in 2,4-D-containing medium, 2,4-D in the medium was analyzed by thin-layer chromatography. In the medium, 2,4-D was decreased during anise callus culture, and fully abolished after 5-day culture. On the other hand, no decrease in 2,4-D was observed in the other Apiaceae species (carrot, fennel, dill, parsley, and coriander). After 7-day culture of anise callus, the medium was collected following removal of the cultured cells and 2,4-D was added to the collected medium. After 10 days of incubation and shaking, 2,4-D was markedly decreased in the medium. However, when the collected medium was heat-treated at 100°C, 2,4-D was detected after 20 days of incubation. Therefore, anise callus has a specific 2,4-D degradation system, in which heat-inactivated secreted molecules may participate.

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Pretreatment free of 2,4-dichlorophenoxyacetic acid improves the differentiation of sugarcane somatic embryos by affecting the hormonal balance and the accumulation of reserves

Reis

Vale

Sousa

et al. 2021

Plant Cell Tiss Organ Cult

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Members of the GH3 Family of Proteins Conjugate 2,4-D and Dicamba with Aspartate and Glutamate

Cited by 13 publications

References 52 publications

Genome-Wide Identification of GH3 Genes in Brassica Oleracea and Identification of a Promoter Region for Anther-Specific Expression of a GH3 Gene

Genome-Wide Identification of GH3 Genes in Brassica Oleracea and Identification of a Promoter Region for Anther-Specific Expression of a GH3 Gene

Anise-cultured cells abolish 2,4-dichlorophenoxyacetic acid in culture medium

Pretreatment free of 2,4-dichlorophenoxyacetic acid improves the differentiation of sugarcane somatic embryos by affecting the hormonal balance and the accumulation of reserves

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