Members of the ethylene signalling pathway are regulated in sugarcane during the association with nitrogen-fixing endophytic bacteria

Cavalcante, Janaína J. V.; Vargas, Claudia; Nogueira, Élcio; Vinagre, Fabiano; Schwarcz, K; Baldani, José Ivo; Ferreira, Paulo Sérgio; Hemerly, Adriana S.

doi:10.1093/jxb/erl242

Cited by 71 publications

(49 citation statements)

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“…The ethylene receptor SCER1 and the transcription factor SCERF2 are induced after inoculation, while SCERF1 is repressed in inoculated plants. These genes showed an opposite expression profile in response to pathogens, and are differentially regulated in sugarcane contrasting BNF genotypes (Cavalcante et al 2007). The data suggest that specific components of ethylene signaling pathways may identify a beneficial endophytic association, modulating plant response to diazotrophic endophytes.…”

Section: Sugarcane Defense Responsesmentioning

confidence: 87%

“…It comprises five distinct classes that share common structural features such as a leucine-rich repeat (LRR) motif or a serine-threonine kinase domain. 280 putative R-genes were identified in the sugarcane EST collection, including in the libraries of plants inoculated with G. diazotrophicus and H. rubrisibalbicans, with the highest percentage of ESTs belonging to Class I (Pto-like) followed by Class II (NBS-LRR) and Class V (LRR-RLK) R-genes (Wanderley- Nogueira et al 2007). Microarray analysis also identified four R-genes responsive to sugarcane association with Herbaspirillum spp.…”

Section: Sugarcane-endophytic Recognitionmentioning

confidence: 99%

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Sugarcane Genetic Controls Involved in the Association with Beneficial Endophytic Nitrogen Fixing Bacteria

Carvalho

Ferreira

Hemerly

2011

Tropical Plant Biol.

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Sugarcane is an economically important culture in Brazil, being the world most important source of sugar and ethanol production. Brazilian sugarcane culture is able to obtain large and significant contributions of nitrogen from plant-associated Biological Nitrogen Fixation (BNF), reducing the use of nitrogen fertilizers and leading to an increase in energy balance of the culture. Different populations of endophytic N 2 -fixing bacteria associated with sugarcane were identified. Besides BNF, these bacteria exhibit growth promoting traits by mechanisms involving nutrient solubilization, plant hormone production and pathogens antagonistic activity. BNF contribution efficiency to sugarcane is controlled by plant and bacteria genotypes as well as environmental conditions. Here, there will be reviewed the advances made in studies on molecular, cellular and physiological mechanisms by which sugarcane controls establishment and efficiency of endophytic association. Transcriptome profile analysis indentified several differentially expressed sugarcane genes during early stages of the association. Regulatory networks involved in various plant processes such as plantmicroorganism recognition, defense, plant hormone signaling, plant growth and nitrogen metabolism were reported to be responsive to endophytic diazotrophic bacteria colonization.The data indicate that sugarcane actively participates in the association with these bacteria. Comprehension of how various sugarcane regulatory mechanisms are coordinated and connected to genotype and environmental signals, in order to control the establishment of a beneficial and endophytic type of association is still a big challenge. Nevertheless, the knowledge being accumulated may guide studies to improve plant association with endophytic diazotrophic bacteria and, possibly, to extend it to other crops.

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Section: Sugarcane Defense Responsesmentioning

confidence: 87%

Section: Sugarcane-endophytic Recognitionmentioning

confidence: 99%

Sugarcane Genetic Controls Involved in the Association with Beneficial Endophytic Nitrogen Fixing Bacteria

Carvalho

Ferreira

Hemerly

2011

Tropical Plant Biol.

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“…The endophytic G.d is being used for biopriming of sugarcane plants because its role as promoting plant growth and as resistance-inducer to the systemic pathogenic bacteria Xanthomonas albilineans (Arencibia et al, 2006). For sugarcane, the expression pattern of a putative ethylene receptor (SCER1) and two putative ERF transcription factors (SCERF1 and SCERF2) show differential response to interactions with pathogenic and beneficial microorganisms, suggesting that it might participate in specific ethylene signaling cascade(s) that can identify a beneficial or pathogenic interaction (Cavalcante et al, 2007). The differential expression of TDFs corresponding to the ethylene-signaling pathway could be also related with improved ability of the G.d infection and support the finding that phenolic metabolites prime sugarcane plants during micropropagation in TIBs .…”

Section: Discussionmentioning

confidence: 99%

Priming and biopriming integrated into the sugarcane micropropagation technology by Temporary Immersion Bioreactors (TIBS)

Bernal¹,

Machado²,

Cortegaza³

et al. 2008

Sugar Tech

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For the first time both priming and biopriming approaches have been integrated into the sugarcane micropropagation technology by temporary immersion bioreactors (TIBs). Sugarcane micropropagation in CO 2 -rich TIBs induces a mixotrophic condition adequate for the production of natural phenolic metabolites. Scaling up has been conducted in the C86-56 and C90-317 commercial genotypes. While phenolics demonstrate to act as priming molecules during the in vitro culture, vitroplantlets growing and shooting in the presence of phenolic metabolites display an enhanced vigour (measure as plant size), emitted functional roots and increase adaptability to the natural environment. Additionally, when combined with the inoculation of the endophytic Gluconacetobacter diazotrophicus during transplanting, a significant improvement of the percentage of survival has been attached through this critical step. Altogether, results indicate a promising potential for diversification of the sugarcane micropropagation industry by the production of useful metabolites as byproducts.

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“…It is known that endophytic diazotrophic bacteria colonize the intercellular spaces and vascular tissues of sugarcane organs, without triggering any symptoms in the plant. The signaling mechanisms are as yet poorly understood but Cavalcante et al (6) suggested that the ethylene signaling pathway may play a role in the establishment of the association between sugarcane and endendophytic diazotrophic bacteria. However, there is little evidence so far that G. diazotrophicus is actually a N2-fixing symbiont of sugarcane or that it even expresses active nitrogenase in plants (13).…”

mentioning

confidence: 99%

Nitrogen Fixation and Translocation in Young Sugarcane (Saccharum officinarum L.) Plants Associated with Endophytic Nitrogen-Fixing Bacteria

et al. 2009

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The tracer15 N2 was used to investigate sites of N2 fixation and the possible translocation of the fixed N. Young sugarcane plants (Saccharum officinarum L.) from a stem cutting were exposed to 15 N2-labeled air in a 500 mL plastic cylinder. Plants fed 15 N2 for 7 days were grown in normal air for a further chase period. After 21 days, about half of the N originating in the stem cutting had been transported to the shoot and roots, suggesting that the cutting played a role in supplying N for growth. After 3 days of feeding, the percentage of N derived from 15 N2 was higher in the roots (2.22%) and stem cutting (0.271%) than the shoot (0.027%). Most of the fixed N was distributed in the 80% ethanolinsoluble fractions in each plant part, and the 15 N fixed either in the roots or in the stem cutting remained there and was not appreciably transported to the shoot. The results were quite different from the fate of fixed N in soybean nodules, which is rapidly transported from nodules to roots and shoots. Key words:15 N2, nitrogen fixation, Saccharum officinarum L., sugarcane roots, translocation Sugarcane (Saccharum officinarum L.) is a tall, perennial grass (family Poaceae, subfamily Panicoide), and is cultivated in tropical and warm-temperate regions between 35°N and 35°S and from sea level to altitudes of 1,000 m in a wide variety of soil types (27). For many years, sugarcane has been used mainly for sugar and for alcohol production. Recently, the use of sugarcane alcohol (ethanol) as an automotive fuel to replace gasoline has rapidly increased (5, 17). In 2006, world production of sugarcane was 1,392 million tons. This is much greater than production levels for the other major crops such as maize (695 million tons), paddy rice (635 million tons), wheat (606 million tons) and potatoes (315 million tons) (http://faostat.fao.org/site/567/default. aspx). Sugarcane production is highest in Brazil (455 million tons), followed by India (281 million tons). In 2006, sugarcane was cropped over an area of 20 million hectares, and the average yield was 68 tons per hectare. Sugarcane is a C4 plant, which has an efficient photosynthetic system, and it can convert up to 2% of incident solar energy into biomass. It grows up to 4 m in height and the thick stem stores a high concentration of sucrose which is present in the expressed juice at between 12 and 20% (w/v).In Brazil, sugarcane crops accumulate between 100 and 200 kg N per hectare per year, while N fertilization rates are relatively low-usually less than 60 kg N per hectare (27). Also, the response of sugarcane crops to N fertilizers is usually very weak or non-existant (5, 27). In some areas of Brazil, sugarcane has been grown continuously for more than 100 years without any N fertilizer being applied at all (10). This circumstantial evidence suggests a high potential for biological nitrogen fixation (BNF) in sugarcane. Using a 15 N dilution technique involving the supply of a 15 N-labeled fertilizer, Urquiaga et al. (32) calculated the contribution of BNF in several cult...

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Members of the ethylene signalling pathway are regulated in sugarcane during the association with nitrogen-fixing endophytic bacteria

Cited by 71 publications

References 50 publications

Sugarcane Genetic Controls Involved in the Association with Beneficial Endophytic Nitrogen Fixing Bacteria

Sugarcane Genetic Controls Involved in the Association with Beneficial Endophytic Nitrogen Fixing Bacteria

Priming and biopriming integrated into the sugarcane micropropagation technology by Temporary Immersion Bioreactors (TIBS)

Nitrogen Fixation and Translocation in Young Sugarcane (Saccharum officinarum L.) Plants Associated with Endophytic Nitrogen-Fixing Bacteria

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