Members of the Arabidopsis HRT/RPP8 Family of Resistance Genes Confer Resistance to Both Viral and Oomycete Pathogens

Cooley, Michael B.; Pathirana, Sudam M.; Wu, Hui‐Ju; Kachroo, Pradeep; Klessig, Daniel F.

doi:10.2307/3870993

Cited by 96 publications

(142 citation statements)

References 58 publications

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“…1E). Final confirmation that the SSI2 gene was isolated came from the demon- (57). Plants were sampled 7 days after inoculation and scored as susceptible if they developed 10 or more sporangiophores per cotyledon.…”

Section: Resultsmentioning

confidence: 99%

A fatty acid desaturase modulates the activation of defense signaling pathways in plants

Kachroo

Shanklin

Shah

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

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379

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Salicylic acid (SA) plays an important role in activating various plant defense responses, including expression of the pathogenesisrelated (PR) genes and systemic acquired resistance. A critical positive regulator of the SA signaling pathway in Arabidopsis is encoded by the NPR1 gene. However, there is growing evidence that NPR1-independent pathways can also activate PR expression and disease resistance. To elucidate the components associated with NPR1-independent defense signaling, we isolated a suppressor of the npr1-5 allele, designated ssi2. The recessive ssi2 mutation confers constitutive PR gene expression, spontaneous lesion formation, and enhanced resistance to Peronospora parasitica.

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Section: Resultsmentioning

confidence: 99%

A fatty acid desaturase modulates the activation of defense signaling pathways in plants

Kachroo

Shanklin

Shah

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

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379

323

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“…Within a cluster of RGAs, there may be more than one gene conferring resistance to different isolates of a specific pathogen or to biologically diverse pathogen taxa (Cooley et al 2000, Van der Vossen et al 2000. Some RGAs are genetically linked to R loci in Arabidopsis (Aarts et al 1998).…”

Section: Discussionmentioning

confidence: 99%

Isolation, genetic variation and expression of TIR-NBS-LRR resistance gene analogs from western white pine (Pinus monticola Dougl. ex. D. Don.)

Liu

Ekramoddoullah

2003

Mol Genet Genomics

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Western white pine ( Pinus monticola Dougl. ex. D. Don., WWP) shows genetic variation in disease resistance to white pine blister rust ( Cronartium ribicola). Most plant disease resistance (R) genes encode proteins that belong to a superfamily with nucleotide-binding site domains (NBS) and C-terminal leucine-rich repeats (LRR). In this work a PCR strategy was used to clone R gene analogs (RGAs) from WWP using oligonucleotide primers based on the conserved sequence motifs in the NBS domain of angiosperm NBS-LRR genes. Sixty-seven NBS sequences were cloned from disease-resistant trees. BLAST searches in GenBank revealed that they shared significant identity to well-characterized R genes from angiosperms, including L and M genes from flax, the tobacco N gene and the soybean gene LM6. Sequence alignments revealed that the RGAs from WWP contained the conserved motifs identified in angiosperm NBS domains, especially those motifs specific for TIR-NBS-LRR proteins. Phylogenic analysis of plant R genes and RGAs indicated that all cloned WWP RGAs can be grouped into one major branch together with well-known R proteins carrying a TIR domain, suggesting they belong to the subfamily of TIR-NBS-LRR genes. In one phylogenic tree, WWP RGAs were further subdivided into fourteen clusters with an amino acid sequence identity threshold of 75%. cDNA cloning and RT-PCR analysis with gene-specific primers demonstrated that members of 10 of the 14 RGA classes were expressed in foliage tissues, suggesting that a large and diverse NBS-LRR gene family may be functional in conifers. These results provide evidence for the hypothesis that conifer RGAs share a common origin with R genes from angiosperms, and some of them may play important roles in defense mechanisms that confer disease resistance in western white pine. Ratios of non-synonymous to synonymous nucleotide substitutions (Ka/Ks) in the WWP NBS domains were greater than 1 or close to 1, indicating that diversifying selection and/or neutral selection operate on the NBS domains of the WWP RGA family.

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“…For example, the Arabidopsis HRT/RPP8 cluster confers resistance to a viral pathogen and an oomycete pathogen (Cooley et al 2000). In potato, the Gpa2 locus for potato cyst nematode resistance also contains Rx1, a gene for potato virus X resistance (Van der Vossen et al 2000).…”

Section: Introductionmentioning

confidence: 99%

PCR Sampling of disease resistance-like sequences from a disease resistance gene cluster in soybean

2002

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Clusters of Resistance-like genes (RLGs) have been identified from a variety of plant species. In soybean, RLG-specific primers and BAC-fingerprinting were used to develop a contig of overlapping BACs for a cluster of RLGs on soybean linkage group J. The resistance genes Rps2 ( Phytophthora stem and root rot) and Rmd-c (powdery mildew) and the ineffective nodulation gene Rj2 were previously mapped to this region of linkage group J. PCR hybridization was used to place two TIR/NBD/LRR cDNAs on overlapping BACs from this contig. Both of the cDNAs were present on BAC 34P7. Fingerprinting of this BAC suggested as many as twelve different RLGs were present. Given the high nucleotide identity shared between cDNAs LM6 and MG13 (>90%), direct sequencing of this region would be difficult. More sequence information was needed about the RLGs within this region before sequencing could be undertaken. By comparing the genomic sequences of cDNAs LM6 and MG13 we identified conserved regions from which oligonucleotide primers specific to BAC 34P7 RLGs could be designed. The nine primer pairs spanned the genomic sequence of LM6 and produced overlapping RLG products upon amplification of BAC 34P7. Amplification products from 12 different RLGs were identified. On average, nucleotide identity between RLG sequences was greater than 95%. Examination of RLG sequences also revealed evidence of additions, deletions and duplications within targeted regions of these genes. Using previously mapped cDNAs we were able to quickly and inexpensively access multiple RLGs within a single specific cluster.

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Members of the Arabidopsis HRT/RPP8 Family of Resistance Genes Confer Resistance to Both Viral and Oomycete Pathogens

Cited by 96 publications

References 58 publications

A fatty acid desaturase modulates the activation of defense signaling pathways in plants

A fatty acid desaturase modulates the activation of defense signaling pathways in plants

Isolation, genetic variation and expression of TIR-NBS-LRR resistance gene analogs from western white pine (Pinus monticola Dougl. ex. D. Don.)

PCR Sampling of disease resistance-like sequences from a disease resistance gene cluster in soybean

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