Members of adenovirus species B utilize CD80 and CD86 as cellular attachment receptors

Abstract

Alternate serotypes of adenovirus (Ad), including Ads of species B, are being explored to circumvent the disadvantages of Ad serotype 5 gene delivery vectors. Whereas the majority of human Ads utilize the Coxsackievirus and adenovirus receptor (CAR), none of the Ad species B use CAR. Ad species B is further divided into two subspecies, B1 and B2, and utilizes at least two classes of receptors: common Ad species B receptors and B2 specific receptors. CD46 has been implicated as a B2-specific receptor. Ad serotype 3 (Ad3), a member of B1, utilizes CD80 and CD86 as cellular attachment receptors. The receptor-interacting Ad fiber-knob domain is highly homologous among species B Ads. We hypothesized that other members of Ad species B may utilize CD80 and CD86 as cellular attachment receptors. All tested species B members showed specific binding to cells expressing CD80 and CD86, and the Ad fiber-knob domain from both B1 and B2 Ad efficiently blocked CD80-and CD86-mediated infection of Ad3 vectors. Members of both B1 and B2 demonstrated CD80-and CD86-specific infection of CHO cells expressing CD80 and CD86. Therefore, all of the members of Ad species B utilize CD80 and CD86 for infection of cells.

Adenoviruses: update on structure and function

“…There have been rather conflicting results in terms of receptors for species B viruses, but it has been claimed that all bind CD46 (Fleischli et al, 2007;Marttila et al, 2005). Ads 3, 7 and 11 also bind the related receptors CD80 and CD86, and it has been claimed that these receptors can also be utilized by all species B adenoviruses (Short et al, 2006). However, contrary results for Ad 3 have been demonstrated, suggesting that there may be receptors other than CD46, CD80 and CD86 (K. Hall and G. E. Blair, personal communication).…”

Infection kinetics of human adenovirus serotype 41 in HEK 293 cells

“…Characteristic features of each species determine cell tropism and pathogenesis, which can result in ocular, respiratory, enteric and urinary tract infections (Cusack 2005, Short et al 2006). All adenoviruses are non-enveloped, double-stranded DNA viruses.…”

Selective Transduction of Mature DC in Human Skin and Lymph Nodes by CD80/CD86-targeted Fiber-modified Adenovirus-5/3

“…As Ad5/3 was reported to bind the B7 family members CD80 and CD86 on in vitro generated human MoDC 8,25, but has also been reported to bind CD4614,16, we decided to examine the targeted surface molecules by Ad5/3 on human skin-DC. MoDC and skin-emigrated DC were incubated with a control-Ig or CTLA4-Ig fusion protein, the latter to prevent CD80 and CD86 binding by the virus.…”