Melting transition of directly linked gold nanoparticle DNA assembly

Sun, Young; Harris, Nolan C.; Kiang, Ching‐Hwa

doi:10.1016/j.physa.2005.01.013

Cited by 30 publications

(35 citation statements)

References 9 publications

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“…In the last 10 years, there has been growing interest in the use of DNA to make nanoscale structures. One version of this has involved using DNA to link together nanoparticles, viruses, and polymeric materials (9)(10)(11)(12)(13)(14). These materials have found use in sensing applications, in part because they exhibit much narrower melting transitions (thermal dehybridization) than occur for the same duplexes in solution (15).…”

Section: Structural and Thermal Propertiesmentioning

confidence: 99%

Using theory and computation to model nanoscale properties

Schatz

2007

Proc. Natl. Acad. Sci. U.S.A.

102

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This article provides an overview of the use of theory and computation to describe the structural, thermodynamic, mechanical, and optical properties of nanoscale materials. Nanoscience provides important opportunities for theory and computation to lead in the discovery process because the experimental tools often provide an incomplete picture of the structure and/or function of nanomaterials, and theory can often fill in missing features crucial to understanding what is being measured. However, there are important challenges to using theory as well, as the systems of interest are usually too large, and the time scales too long, for a purely atomistic level theory to be useful. At the same time, continuum theories that are appropriate for describing larger-scale (micrometer) phenomena are often not accurate for describing the nanoscale. Despite these challenges, there has been important progress in a number of areas, and there are exciting opportunities that we can look forward to as the capabilities of computational facilities continue to expand. Some specific applications that are discussed in this paper include: self-assembly of supramolecular structures, the thermal properties of nanoscale molecular systems (DNA melting and nanoscale water meniscus formation), the mechanical properties of carbon nanotubes and diamond crystals, and the optical properties of silver and gold nanoparticles. molecular dynamics ͉ nanomaterials ͉ nanoparticle ͉ plasmon ͉ self-assembly N anoscience deals with the behavior of matter on length scales where a large number of atoms play a role, but where the system is still small enough that the material does not behave like bulk matter. For example, a 5-nm gold particle, which contains on the order of 10 5 atoms, absorbs light strongly at 520 nm, whereas bulk gold is reflective at this wavelength and small clusters of gold atoms have absorption at shorter wavelengths. The special properties associated with nanoscale systems like this have provided both challenges and opportunities for the use of theory and computation to play a role in the discovery process. The challenges arise from the fact that most theories that describe the properties of matter by using first-principles approaches in which all atoms (and even all electrons in these atoms) are explicitly described are close to or (more often) beyond their capability to describe such systems, even using the largest computer available. At the same time, continuum theories, which play such a useful role for micrometer-scale systems, are sometimes incapable of describing nanoscale properties due to incomplete incorporation of the underlying physics in the size-dependent materials parameters. However, the opportunities for theory to play a role are significant, as nanoscience often provides the smallest systems that are amenable to study using methods that involve macroscopic manipulation of nanoscale structures. Thus, it is possible to measure the structure and thermal properties of a single supramolecular assembly, observe the thermal ...

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Section: Structural and Thermal Propertiesmentioning

confidence: 99%

Using theory and computation to model nanoscale properties

Schatz

2007

Proc. Natl. Acad. Sci. U.S.A.

102

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“…DNA gold nanoparticle probes were synthesized using previously described methods [2]. DNA-gold aggregates were formed by mixing probe DNA particles with linker DNA [2][3][4]. Upon the addition of linker DNA, the solutions were allowed to stand at 4 °C for several days in order to achieve maximum network aggregation.…”

Section: Nih-pa Author Manuscriptmentioning

confidence: 99%

“…DNA-linked nanoparticle assemblies are a novel system in which gold nanoparticles are chemically affixed to known DNA sequences to create DNA "probes" with the capability to self-assemble into aggregates [1][2][3][4][5]. The interaction potential between these colloids are tunable and controllable, which makes these multicomponent complex fluids particularly suitable for studying the link between the interaction potential and phase behavior [6,7].…”

Section: Introductionmentioning

confidence: 99%

“…Recent theory has sought to explain these observations [6,23,24]. More study is necessary to fully understand the underlying mechanisms affecting the phase behavior of this system.Here we report experimental observations of the effects of disorder on the melting temperature of the DNA-linked nanoparticle assemblies [2,3]. The basic building block is illustrated in Fig.…”

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confidence: 99%

“…Linker DNA resulting in unequal DNA duplex lengths introduces disorder and lowers the melting temperature of the nanoparticle system. Comparison with free DNA thermodynamics shows that such an anomalous zigzag pattern does not exist for free DNA duplex melting, which suggests that the disorder introduced by unequal DNA duplex lengths results in this unusual collective behavior of DNA-linked nanoparticle assemblies.DNA-linked nanoparticle assemblies are a novel system in which gold nanoparticles are chemically affixed to known DNA sequences to create DNA "probes" with the capability to self-assemble into aggregates [1][2][3][4][5]. The interaction potential between these colloids are tunable and controllable, which makes these multicomponent complex fluids particularly suitable for studying the link between the interaction potential and phase behavior [6,7].…”

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Disorder in DNA-Linked Gold Nanoparticle Assemblies

Harris

Kiang

2005

Phys. Rev. Lett.

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We report experimental observations on the effect of disorder on the phase behavior of DNA-linked nanoparticle assemblies. Variation in DNA linker lengths results in different melting temperatures of the DNA-linked nanoparticle assemblies. We observed an unusual trend of a nonmonotonic "zigzag" pattern in the melting temperature as a function of DNA linker length. Linker DNA resulting in unequal DNA duplex lengths introduces disorder and lowers the melting temperature of the nanoparticle system. Comparison with free DNA thermodynamics shows that such an anomalous zigzag pattern does not exist for free DNA duplex melting, which suggests that the disorder introduced by unequal DNA duplex lengths results in this unusual collective behavior of DNA-linked nanoparticle assemblies.DNA-linked nanoparticle assemblies are a novel system in which gold nanoparticles are chemically affixed to known DNA sequences to create DNA "probes" with the capability to self-assemble into aggregates [1][2][3][4][5]. The interaction potential between these colloids are tunable and controllable, which makes these multicomponent complex fluids particularly suitable for studying the link between the interaction potential and phase behavior [6,7]. Similar colloidal phase transitions have also been used to detect the protein interactions at membrane surfaces [8]. On the other hand, the dynamics of DNA melting and hybridization in DNA replication and transcription is also a subject of intense investigation [9,10].The change in optical property upon aggregation makes DNA-linked nanoparticle systems a potential tool in future DNA detection technology [11,12]. DNA detection is important in medical research for applications such as detection of genetic diseases, RNA profiling, and biodefense [13][14][15][16][17]. The DNA-linked nanoparticle detection systems utilize the sequencedependent hybridization of DNA for accuracy and the optical properties of colloidal gold for sensitivity to create a DNA detection method that changes color upon the introduction of a specific DNA sequence. Study of these DNA-gold nanoparticle assemblies is warranted to gain a fundamental understanding of DNA hybridization in confined geometries, as well as to probe the potential of this and similar systems for practical applications in biotechnology [18,19].Much like other colloidal suspensions [20][21][22], the DNA-gold nanoparticle system has been shown to exhibit interesting phase behavior. The assembly melting temperature is dependent upon parameters such as particle size, DNA composition, and electrolyte concentrations [1,2]. Recent theory has sought to explain these observations [6,23,24]. More study is necessary to fully understand the underlying mechanisms affecting the phase behavior of this system.Here we report experimental observations of the effects of disorder on the melting temperature of the DNA-linked nanoparticle assemblies [2,3]. The basic building block is illustrated in Fig. 1. Disorder in the DNA duplex length was introduced by choosing linker...

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2015

DNA Engineered Noble Metal Nanoparticles

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Melting transition of directly linked gold nanoparticle DNA assembly

Cited by 30 publications

References 9 publications

Using theory and computation to model nanoscale properties

Using theory and computation to model nanoscale properties

Disorder in DNA-Linked Gold Nanoparticle Assemblies

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