MELOGEN: an EST database for melon functional genomics

Abstract

Background: Melon (Cucumis melo L.) is one of the most important fleshy fruits for fresh consumption. Despite this, few genomic resources exist for this species. To facilitate the discovery of genes involved in essential traits, such as fruit development, fruit maturation and disease resistance, and to speed up the process of breeding new and better adapted melon varieties, we have produced a large collection of expressed sequence tags (ESTs) from eight normalized cDNA libraries from different tissues in different physiological conditions.

Gene mining a marama bean expressed sequence tags (ESTs) database: Embryonic seed development genes and microsatellite marker identification

“…ESTs are short (200-800 nucleotide bases in length); unedited, randomly selected single-pass sequence reads derived from cDNA libraries (Nagaraj et al, 2006). EST libraries have been developed for plant species such as tomato, apple, rice, grape and citrus (Gonzalez-Ibeas et al, 2007). However, amongst the comprehensive ones are Arabidopsis thaliana and Oryzae sativa which are the common models for analysis (Gonzalez-Ibeas et al, 2007).…”

“…EST libraries have been developed for plant species such as tomato, apple, rice, grape and citrus (Gonzalez-Ibeas et al, 2007). However, amongst the comprehensive ones are Arabidopsis thaliana and Oryzae sativa which are the common models for analysis (Gonzalez-Ibeas et al, 2007). Bioinformatics tools can be used to identify and dissect biological processes that are of great technological importance such as flavor development and fruit ripening through the analysis of ESTs (Gonzalez-Ibeas et al, 2007).…”

“…However, amongst the comprehensive ones are Arabidopsis thaliana and Oryzae sativa which are the common models for analysis (Gonzalez-Ibeas et al, 2007). Bioinformatics tools can be used to identify and dissect biological processes that are of great technological importance such as flavor development and fruit ripening through the analysis of ESTs (Gonzalez-Ibeas et al, 2007). Gene mining can be used to select candidate genes that are associated with traits of interest (Frank et al, 2004;Higgs and Attwood, 2005).…”

“…Gene mining can be used to select candidate genes that are associated with traits of interest (Frank et al, 2004;Higgs and Attwood, 2005). The EST collections can also be used to develop microarrays to identify genes expressed during plant developmental stages and/or responding to environmental stimuli as well as to gain deeper understanding of the common regulatory mechanisms amongst diverse fruit species and ripening physiological patterns (Gonzalez-Ibeas et al, 2007;Fei et al, 2004). Some previous studies have used this analysis to identify genes involved in fruit ripening and pathogen defense (Gonzalez-Ibeas et al, 2007).…”

Respuesta de los frutos cítricos a las bajas temperaturas: estudio mediante micromatrices

“…Ejemplos de esas aplicaciones se pueden observar en manzano (Newcomb et al, 2006), en melón (Gonzalez-Ibeas et al, 2007), y en cítricos (Luro et al, 2008). Rudd et al (2005) demostraron la viabilidad de la predicción de marcadores moleculares (SSRs, SNPs) en más de 50 especies vegetales a partir de ESTs (PlantMarkers, http:/markers.btk.fi/).…”