Melissa officinalis L. ameliorates oxidative stress and inflammation and upregulates Nrf2/HO-1 signaling in the hippocampus of pilocarpine-induced rats

Allah, Hagar N Abd; Abdul-Hamid, Manal; Mahmoud, Ayman M.; Abdel-Reheim, Eman S.

doi:10.1007/s11356-021-15825-y

Cited by 17 publications

(14 citation statements)

References 70 publications

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“…These results support the favorable bioavailability and effectiveness of Relissa™ for its neuroprotective activity against oxidative stress damage. Moreover, the antioxidant effect of MO extracts evaluated in the present study is in agreement with previously reported studies ( Dastmalchi et al, 2008 ; López et al, 2009 ; Özkol et al, 2011 ; Lin et al, 2012 ; Martins et al, 2012 ; Ghazizadeh et al, 2020 ; Abd Allah et al, 2022 ; Abo-Zaid et al, 2023 ).…”

Section: Discussionsupporting

confidence: 93%

“…Extensive preclinical and clinical studies have suggested a therapeutic effect of MO extract supplementation as an efficacious and safe therapy to alleviate emotional distress conditions such as low mood, anxiety, and stress and improve sleep disorders ( Kennedy et al, 2002 ; Akhondzadeh et al, 2003 ; Kennedy et al, 2003 ; Kennedy et al, 2004 ; Kennedy et al, 2006 ; López et al, 2009 ; Cases et al, 2011 ; Alijaniha et al, 2015 ; Arceusz et al, 2015 ; Soltanpour et al, 2019 ; Noguchi-Shinohara et al, 2020 ). Reported evidence suggest that the active chemical constituents of MO extract possess diverse psycho- and neuropharmacological properties, including antidepressant ( Awad et al, 2007 ; Awad et al, 2009 ; López et al, 2009 ; Sahin et al, 2016 ), antioxidative stress ( Özkol et al, 2011 ; Martins et al, 2012 ; Ghazizadeh et al, 2020 ; Abd Allah et al, 2022 ; Abo-Zaid et al, 2023 ), and anti-inflammatory effects ( Baldwin et al, 2011 ).…”

Section: Introductionmentioning

confidence: 99%

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An in vitro analysis of an innovative standardized phospholipid carrier-based Melissa officinalis L. extract as a potential neuromodulator for emotional distress and related conditions

Kara,

Sahin,

Rabbani

et al. 2024

Front. Mol. Biosci.

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Background:Melissa officinalis L. (MO), commonly known as lemon balm, a member of the mint family, is considered a calming herb. In various traditional medicines, it has been utilized to reduce stress and anxiety and promote sleep. A growing body of clinical evidence suggests that MO leaf extract supplementation possesses considerable neuropharmacological properties. However, its possible mechanism of action largely remains unknown.Objective: In the present in vitro studies, we comparatively investigated the central nervous system (CNS)-calming and antioxidative stress properties of an innovative standardized phospholipid carrier-based (Phytosome™) MO extract (Relissa™) vs. an unformulated dry MO extract.Methods: The neuropharmacological effect of the extract was studied in the anti-depressant enzymes γ-aminobutyrate transaminase (GABA-T) and monoamine oxidase A (MAO-A) assays and SH-SY5Y cells brain-derived neurotrophic factor (BDNF) expression assay. The neuroprotective effect of the extract against oxidative stress was assessed in SH-SY5Y cell-based (H2O2-exposed) Total Antioxidant Status (TAS) and Total Reactive Oxygen Species (ROS) assays. The cytotoxic effect of the extract was evaluated using MTT and LDH assays. The extract antioxidant effect was also evaluated in cell-free chemical tests, including TEAC-ABTS, DPPH, Ferric Reducing Antioxidant Power (FRAP), Oxygen Radical Antioxidant Capacity (ORAC), and Hydroxyl Radical Antioxidant Capacity (HORAC) assays.Results: Relissa™ exhibited high GABA-T inhibitory activity, IC50 (mg/mL) = 0.064 vs. unformulated dry MO extract, IC50 (mg/mL) = 0.27. Similar inhibitory effects were also observed for MAO-A. Relissa™ demonstrated an improved neuroprotective antioxidant effect on SH-SY5Y cells against H2O2-induced oxidative stress. Compared to unformulated dry MO extract, Relissa™ exerted high protective effect on H2O2-exposed SH-SY5Y cells, leading to higher cells BDNF expression levels. Moreover, cell-free chemical tests, including TEAC-ABTS, DPPH radical scavenging, FRAP, ORAC, and HORAC assays, validated the improved antioxidant effect of Relissa™ vs. unformulated dry MO extract.Conclusion: The results of the present study support the neuromodulating and neuroprotective properties of Relissa™, and its supplementation may help in the amelioration of emotional distress and related conditions.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

An in vitro analysis of an innovative standardized phospholipid carrier-based Melissa officinalis L. extract as a potential neuromodulator for emotional distress and related conditions

Kara,

Sahin,

Rabbani

et al. 2024

Front. Mol. Biosci.

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“…6 Melissa bitkisi özütünün çalışmamızda kullandığımız PGE2 parametresinin düzeyini azalttığı bildirilmiştir. 23 NO temizleyici etkisi incelendiğinde Kaempferolün izoflavonlardan etkin olduğu ve ayrıca LPS ile indüklenmiş RAW 264.7 makrofajlarında iNOS üzerine inhibe edici etki göstermiştir. 24 Ayrıca, LPS ile indüklenmiş J774.2 hücrelerinde nitrit birikiminin inhibisyonunda kaempferolün kuersetine oranla daha aktif olduğu bildirilmiştir.…”

Section: Tartışma Ve Sonuçunclassified

Tıbbi Bitkilerde Bulunan Kaempferol’ün LPS ile İndüklenmiş THP-1 Makrofajları Üzerine Etkisi

Yıldırım

2023

Mersin Üniversitesi Tıp Fakültesi Lokman Hekim Tıp Tarihi Ve Folklorik Tıp Dergisi

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Giriş: İnflamasyon, immün sisteminin toksik bileşikler, hasarlı hücreler ve patojenlerinde içinde yer aldığı çeşitli faktörler ile tetiklediğinde verdiği biyolojik bir cevaptır. Tıbbi bitkilerden izole edilen etkin maddeler, inflamatuar hastalıkların tedavisinde yeni bir terapötik strateji olarak kullanılırken dikkatle seçilmelidir. Kaempferol tıbbi bitkilerde sebzelerde meyvelerde bolca bulunan bir polifenoldur. Kaempferol anti-kanser, anti-viral, anti-bakteriyel özelliklere sahiptir. Amaç: Bu çalışmada amaç LPS ile indüklenmiş THP-1 makrofaj hücrelerinde kaempferolün hücre canlılığı ve inflamasyonun tedavisi için yapılan çalışmalarda hedef molekül olan NO ve PGE2 düzeyleri üzerine etkisini incelemektir. Bulgular: 2022 yılında yapmış olduğumuz çalışmada LPS ile indüklenmiş THP-1 makrofaj hücrelerine 2,5, 5, 7,5, 10 µM konsantrasyonunda kaempferol uygulandı. Hücre canlılığı, PGE2 düzeyi ve NO üretimi incelendi. Farklı dozlarda kaempferol uygulaması THP-1 makrofaj hücrelerinde hücre canlılığını azaltmadığı belirlendi. Ayrıca LPS ile indüklenen THP-1 makrofaj hücrelerinde 2,5 µM kaempferol uygulaması % NO düzeyini ve 7,5 µM kaempferol uygulaması PGE2 düzeyini istatistiksel olarak anlamlı azaltmıştır. Tartışma ve sonuç: 7,5 µM kaempferol uygulaması makrofaj hücrelerinin proliferasyonu negatif etki göstermeyip hem NO düzeyini hemde PGE2 düzeni azaltıp iyi anti inflamatuvar etki göstermektedir.

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“…Similarly, methanol and aqueous extracts of M. officinalis possessed a significant protective effect on hydrogen peroxide-induced toxicity in PC12 cells mainly due to monoamine oxidase inhibition [66]. In addition, the effects of an ethanol extract of M. officinalis were tested in the hippocampus of treated rats, as a potential model of epilepsy [67]. In particular, the antioxidative and anti-inflammatory activity of the extract orally administered at 250 mg/kg impacted positively on Nrf2/HO-1 signaling pathway, Na + /K + -ATPase activity, and GABA while glutamate and acetylcholine diminished and reduced neuronal loss, adding a new potential beneficial effect (anti-epileptic) of M. officinalis to its wide uses.…”

Section: Neuroprotective Propertiesmentioning

confidence: 99%

Correction: Wissam Zam et al. An Updated Review on The Properties of Melissa officinalis L.: Not Exclusively Anti-anxiety. Frontiers in Bioscience-Scholar. 2022; 14: 16

Zam¹,

Quispe²,

Sharifi‐Rad³

et al. 2022

Front. Biosci. (Schol Ed)

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Melissa officinalis L. is a plant of the Lamiaceae family known in numerous countries for its medicinal activities. This plant has been used since ancient times to treat different disorders, including gastrointestinal, cardiovascular, neurological, psychological conditions. M. officinalis contains several phytochemicals such as phenolic acids, flavonoids, terpenoids, and many others at the basis of its pharmacological activities. Indeed, the plant can have antioxidant, anti-inflammatory, antispasmodic, antimicrobial, neuroprotective, nephroprotective, antinociceptive effects. Given its consolidated use, M. officinalis has also been experimented with clinical settings, demonstrating interesting properties against different human diseases, such as anxiety, sleeping difficulties, palpitation, hypertension, depression, dementia, infantile colic, bruxism, metabolic problems, Alzheimer's disease, and sexual disorders. As for any natural compound, drug, or plant extract, also M. officinalis can have adverse effects, even though the reported events are very rare and the plant can be considered substantially safe. This review has been prepared with a specific research strategy, interrogating different databases with the keyword M. officinalis. Moreover, this work analyzes the properties of this plant updating currently available literature, with a special emphasis on human studies.

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Melissa officinalis L. ameliorates oxidative stress and inflammation and upregulates Nrf2/HO-1 signaling in the hippocampus of pilocarpine-induced rats

Cited by 17 publications

References 70 publications

An in vitro analysis of an innovative standardized phospholipid carrier-based Melissa officinalis L. extract as a potential neuromodulator for emotional distress and related conditions

An in vitro analysis of an innovative standardized phospholipid carrier-based Melissa officinalis L. extract as a potential neuromodulator for emotional distress and related conditions

Tıbbi Bitkilerde Bulunan Kaempferol’ün LPS ile İndüklenmiş THP-1 Makrofajları Üzerine Etkisi

Correction: Wissam Zam et al. An Updated Review on The Properties of Melissa officinalis L.: Not Exclusively Anti-anxiety. Frontiers in Bioscience-Scholar. 2022; 14: 16

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