Melioidosis in Mauritius

Issack, Mohammad Iqbal; Bundhun, Chundr Dutt; Gokhool, Hemraj

doi:10.3201/eid1101.040605

Cited by 29 publications

(22 citation statements)

References 10 publications

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“…Several anecdotal case reports and small case series [4][5][6][7][8][9][10][11][12][13] have been reported lately from East and West Africa, as well as on the presence of B. pseudomallei in the environment [14,15] and in animals [16][17][18]. This is raising questions about this infectious agent's distribution and reservoir across the continent, as well as about the clinical relevance of melioidosis in Africa.…”

Section: Introductionmentioning

confidence: 98%

Melioidosis in Africa: Should we be Looking more Closely?

et al. 2015

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Melioidosis is a life-threatening infection caused by the Gram-negative bacterium Burkholderia pseudomallei, mainly found in Southeast Asia. Recently, African foci have been identified, although reports remain mostly anecdotal. In Africa, multiple febrile diseases have been erroneously attributed to malaria in the past, and many cases of fever remain mis- or undiagnosed. Vigilance for previously under-recognized pathogens may enhance our understanding of disease epidemiology and facilitate improvement of patient care. Melioidosis may be such a condition. We summarize data on melioidosis in Africa and discuss the future directions for epidemiological, clinical and bacteriological studies. We conclude that searching for old bugs in new places is no academic treasure hunt but a clinically relevant activity to pursue.

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Section: Introductionmentioning

confidence: 98%

Melioidosis in Africa: Should we be Looking more Closely?

et al. 2015

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“…Soil sampling has not been performed across Asia, but cases of disease are an indicator of B. pseudomallei in the environment. Melioidosis in indigenous people or in returning travelers has been reported for Sri Lanka [20], southern India [21,22], the southwest Indian Ocean island of Mauritius [23], Myanmar [24], and Indonesia [25]. B. pseudomallei has been identified in soil samples in Malaysia, where disease is well recognized [26][27][28].…”

Section: Discussionmentioning

confidence: 99%

Melioidosis in 6 Tsunami Survivors in Southern Thailand

Chierakul

Winothai²,

Wattanawaitunechai³

et al. 2005

Clinical Infectious Diseases

105

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“…Melioidosis is an emerging infectious disease generally considered endemic to Southeast Asia and Northern Australia (12). Positive diagnoses in many tropical countries around the world have expanded the global awareness of melioidosis (3,15,24,25,28,35,39,42,52). In contrast to the ubiquitous nature of B. pseudomallei, B. mallei is also a highly infectious agent causing glanders, a predominantly equine disease (34,50).…”

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confidence: 99%

Glyphosate Resistance as a Novel Select-Agent-Compliant, Non-Antibiotic-Selectable Marker in Chromosomal Mutagenesis of the Essential Genes asd and dapB of Burkholderia pseudomallei

Norris¹,

Kang²,

Lu³

et al. 2009

Appl Environ Microbiol

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Genetic manipulation of the category B select agents Burkholderia pseudomallei and Burkholderia mallei has been stifled due to the lack of compliant selectable markers. Hence, there is a need for additional select-agentcompliant selectable markers. We engineered a selectable marker based on the gat gene (encoding glyphosate acetyltransferase), which confers resistance to the common herbicide glyphosate (GS). To show the ability of GS to inhibit bacterial growth, we determined the effective concentrations of GS against Escherichia coli and several Burkholderia species. Plasmids based on gat, flanked by unique flip recombination target (FRT) sequences, were constructed for allelic-replacement. Both allelic-replacement approaches, one using the counterselectable marker pheS and the gat-FRT cassette and one using the DNA incubation method with the gat-FRT cassette, were successfully utilized to create deletions in the asd and dapB genes of wild-type B. pseudomallei strains. The asd and dapB genes encode an aspartate-semialdehyde dehydrogenase (BPSS1704, chromosome 2) and dihydrodipicolinate reductase (BPSL2941, chromosome 1), respectively. Mutants unable to grow on media without diaminopimelate (DAP) and other amino acids of this pathway were PCR verified. These mutants displayed cellular morphologies consistent with the inability to cross-link peptidoglycan in the absence of DAP. The B. pseudomallei 1026b ⌬asd::gat-FRT mutant was complemented with the B. pseudomallei asd gene on a site-specific transposon, mini-Tn7-bar, by selecting for the bar gene (encoding bialaphos/PPT resistance) with PPT. We conclude that the gat gene is one of very few appropriate, effective, and beneficial compliant markers available for Burkholderia select-agent species. Together with the bar gene, the gat cassette will facilitate various genetic manipulations of Burkholderia select-agent species.

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Melioidosis in Mauritius

Cited by 29 publications

References 10 publications

Melioidosis in Africa: Should we be Looking more Closely?

Melioidosis in Africa: Should we be Looking more Closely?

Melioidosis in 6 Tsunami Survivors in Southern Thailand

Glyphosate Resistance as a Novel Select-Agent-Compliant, Non-Antibiotic-Selectable Marker in Chromosomal Mutagenesis of the Essential Genes asd and dapB of Burkholderia pseudomallei

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