Melatonin suppresses apoptosis and stimulates progesterone production by bovine granulosa cells via its receptors (MT1 and MT2)

Wang, S.-J.; Liu, W.-J.; Wu, Chaofeng; Ma, Fei; Ahmad, Siti Zu Nurain; Liu, Biao; Han, Li; Jiang, Xunping; Zhang, S.-J.; Yang, Liguo

doi:10.1016/j.theriogenology.2012.06.019

Cited by 80 publications

(85 citation statements)

References 35 publications

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“…Melatonin-binding sites have been detected in granulosa-luteal cells in humans (Yie et al 1995, Woo et al 2001) and in cattle (Wang et al 2012). Previous studies demonstrated that melatonin significantly increased P 4 production in in vitro-cultured human and bovine granulosa cells (Webley & Luck 1986).…”

Section: Discussionmentioning

confidence: 98%

“…ROS also disrupt the plasma membrane of luteal cells because of lipid peroxidation and, as a consequence, membrane damage is often seen in the regressing corpus luteum (Gatzuli et al 1991, Vega et al 1995. In this way, melatonin prevented apoptosis in in vitro-cultured bovine granulosa cells (Wang et al 2012).…”

Section: Discussionmentioning

confidence: 99%

“…However, the presence of high melatonin levels in follicular fluid (Brzezinski et al 1987) and the presence of receptors in granulosa cells (Yie et al 1995, Niles et al 1999, Woo et al 2001, Soares et al 2003a, Wang et al 2012, Barros et al 2013 have suggested a role of melatonin on ovarian function. This hypothesis has been confirmed by recent studies reporting strong evidence for melatonin action on the ovary, such as modulation of ovarian steroidogenesis (mainly progesterone (P 4 ) production; Taketani et al 2011) and contribution in the maintenance of a proper follicular structure and function (Soares et al 2003a,b, Barros et al 2013, Maganhin et al 2013.…”

Section: Introductionmentioning

confidence: 99%

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Melatonin deprival modifies follicular and corpus luteal growth dynamics in a sheep model

Manca¹,

Manunta²,

Spezzigu³

et al. 2014

Reproduction

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This study assessed the effect of melatonin deprival on ovarian status and function in sheep. Experimental procedures were carried out within two consecutive breeding seasons. Animals were divided into two groups: pinealectomised (nZ6) and sham-operated (nZ6). The completeness of the pineal gland removal was confirmed by the plasma concentration of melatonin. Ovarian status was monitored by ovarian ultrasonography for 1 year to study reproductive seasonality. Follicular and corpus luteal growth dynamics were assessed during an induced oestrous cycle. As the effects of melatonin on the ovary may also be mediated by its antioxidant properties, plasma Trolox equivalent antioxidant capacity (TEAC) was determined monthly for 1 year. Pinealectomy significantly extended the breeding season (310G24.7 vs 217.5G24.7 days in controls; P!0.05). Both pinealectomised and sham-operated ewes showed a well-defined wave-like pattern of follicle dynamics; however, melatonin deficiency caused fewer waves during the oestrous cycle (4.3G0.2 vs 5.2G0.2; P!0.05), because waves were 1 day longer when compared with the controls (7.2G0.3 vs 6.1G0.3; P!0.05). The mean area of the corpora lutea (105.4G5.9 vs 65.4G5.9 mm 2 ; P!0.05) and plasma progesterone levels (7.1G0.7 vs 4.9G0.6 ng/ml; P!0.05) were significantly higher in sham-operated ewes compared with pinealectomised ewes. In addition, TEAC values were significantly lower in pinealectomised ewes compared with control ones. These data suggest that melatonin, besides exerting its well-known role in the synchronisation of seasonal reproductive fluctuations, influences the growth pattern of the follicles and the steroidogenic capacity of the corpus luteum. Free Italian abstractAn Italian translation of this abstract is freely available at

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Melatonin deprival modifies follicular and corpus luteal growth dynamics in a sheep model

Manca¹,

Manunta²,

Spezzigu³

et al. 2014

Reproduction

View full text Add to dashboard Cite

show abstract

“…Some studies showed that melatonin was present in follicular fluid (Brzezinski et al, 1987), and melatonin receptors MT1 and MT2 are expressed in ovary. Melatonin in follicular improves the growth and maturation of COCs (Zhao et al, 2016; Kang et al, 2009) and induces the secretion of progesterone and estrogen in granulosa cells and luteal cells (Wang et al, 2012a; Battista, Rexroad Jr & Condon, 1987). Estradiol and progesterone are essential for follicular development and oocyte maturation (Okamoto et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

The effects of melatonin on bovine uniparental embryos developmentin vitroand the hormone secretion of COCs

Wang

Bao-ru

Liu

et al. 2017

PeerJ

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Melatonin is a unique multifunctional molecule that mediates reproductive functions in animals. In this study, we investigated the effects of melatonin on bovine parthenogenetic and androgenetic embryonic development, oocyte maturation, the reactive oxygen species (ROS) levels in parthenogenetic and androgenetic embryos and cumulus—oocyte complexes (COCs) hormone secretion with melatonin supplementation at four concentrations (0, 10, 20, and 30 pmol/mL), respectively. The results showed that melatonin significantly promoted the rates of bovine parthenogenetic and androgenetic embryonic cleavage and morula and blastocysts development (P < 0.05). The rate of cleavage was higher in the androgenetic embryo than that in the parthenogenetic embryo. Compared with the parthenogenetic embryos, the androgenetic embryos had a poor developmental competence from morula to blastocyst stage. Moreover, the levels of ROS were significantly lower in the parthenogenetic and androgenetic embryoes with melatonin-treated group than that of the control group (P < 0.05). Melatonin supplemented significantly increased the maturation rate of oocyte in vitro (P < 0.05). More importantly, melatonin significantly promoted the secretion of progesterone and estradiol by COCs (P < 0.05). To reveal the regulatory mechanism of melatonin on steroids synthesis, we found that steroidogenic genes (CYP11A1, CYP19A1 and StAR) were upregulated, suggesting that melatonin regulated estradiol and progesterone secretion through mediating the expression of steroidogenic genes (CYP11A1, CYP19A1 and StAR). In addition, MT1 and MT2 were identified in bovine early parthenogenetic and androgenetic embryos using western blot. It could be concluded that melatonin had beneficial effects on bovine oocyte in vitro maturation, COC hormone secretion, early development of subsequent parthenogenetic and androgenetic embryos. It is inferred that melatonin could be used to enhance the efficiency of in vitro developed embryos.

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“…As mentioned above, melatonin signals through two types of melatonin receptors, MT1 and MT2 in mammals [124]. In fact, several studies supported that melatonin receptors are present in the oocytes and granulosa cells of various species ovary including human [154,160,161,162,163]. Melatonin receptors are G protein-coupled receptors, which play an important role in various cellular processes and drug responses [125].…”

Section: Melatonin and Other Adjuvant Agents For Protection Againsmentioning

confidence: 99%

Melatonin and Fertoprotective Adjuvants: Prevention against Premature Ovarian Failure during Chemotherapy

Jang

Hong

Choi

2017

IJMS

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Premature ovarian failure is one of the side effects of chemotherapy in pre-menopausal cancer patients. Preservation of fertility has become increasingly important in improving the quality of life of completely recovered cancer patients. Among the possible strategies for preserving fertility such as ovarian tissue cryopreservation, co-treatment with a pharmacological adjuvant is highly effective and poses less of a burden on the human body. Melatonin is generally produced in various tissues and acts as a universally acting antioxidant in cells. Melatonin is now more widely used in various biological processes including treating insomnia and an adjuvant during chemotherapy. In this review, we summarize the information indicating that melatonin may be useful for reducing and preventing premature ovarian failure in chemotherapy-treated female patients. We also mention that many adjuvants other than melatonin are developed and used to inhibit chemotherapy-induced infertility. This information will give us novel insights on the clinical use of melatonin and other agents as fertoprotective adjuvants for female cancer patients.

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Melatonin suppresses apoptosis and stimulates progesterone production by bovine granulosa cells via its receptors (MT1 and MT2)

Cited by 80 publications

References 35 publications

Melatonin deprival modifies follicular and corpus luteal growth dynamics in a sheep model

Melatonin deprival modifies follicular and corpus luteal growth dynamics in a sheep model

The effects of melatonin on bovine uniparental embryos developmentin vitroand the hormone secretion of COCs

Melatonin and Fertoprotective Adjuvants: Prevention against Premature Ovarian Failure during Chemotherapy

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