Melatonin slightly alleviates the effect of heat shock on bovine oocytes and resulting blastocysts

Yaacobi-Artzi, Shira; Shimoni, Chen; Kalo, Dorit; Hansen, Peter J.; Roth, Zvi

doi:10.1016/j.theriogenology.2020.09.039

Cited by 17 publications

(16 citation statements)

References 63 publications

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“…This is similar to our results, after melatonin treatment, the content of MDA decreased and the activities of SOD and GSH‐PX increased in beef heifers compared to those of the CTR group. Combined with previous studies on the melatonin alleviated the deleterious effects of heat stress on bovine oocytes developmental competence, 35 it suggests that melatonin treatment increases the antioxidant levels in beef heifers.…”

Section: Discussionsupporting

confidence: 58%

Two melatonin treatments improve the conception rate after fixed‐time artificial insemination in beef heifers following synchronisation of oestrous cycles using the CoSynch‐56 protocol

Gao

et al. 2021

Aust Veterinary J

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Objective This study investigated the effect of melatonin administration in combination with the CoSynch‐56 protocol on the conception rate after artificial insemination (AI) in beef heifers. Methods Eighty‐six beef heifers were allocated to four treatments in combination with CoSynch‐56 treatment. All heifers, excluding the control group (CTR = 25), were injected with melatonin on day 1. The melatonin (MT = 20) and MT + human chorionic gonadotropin (MT + hCG = 21) groups received no further treatment with melatonin. Each heifer was treated with gonadotropin‐releasing hormone (GnRH) on day 4, prostaglandin F2α (PGF2α) on day 11, and GnRH and AI 56 h later. The fourth group (2MT = 20) was injected again with melatonin concurrent with AI, and the MT + hCG group received hCG on day 19. Pregnancy diagnosis was performed using transrectal ultrasonography 45 days after AI and blood samples were collected via caudal venipuncture on days 0–1, 14–15, 24 and 59. Concentrations of progesterone (P4) and the activities of oxidative stress‐related enzymes were determined using enzyme‐linked immunosorbent assay. Results The conception rate was greater in the 2MT group (75%) than in the other groups, while there was no significant difference among the CTR (44%), MT (45%) and MT + hCG (38.1%) groups. Treatment with melatonin increased superoxide dismutase and glutathione peroxidase activities and decreased malondialdehyde concentrations but did not significantly affect the concentration of P4. Conclusion Our results indicate that the administration of melatonin twice during the CoSynch‐56 protocol may increase conception rates in beef heifers.

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Section: Discussionsupporting

confidence: 58%

Two melatonin treatments improve the conception rate after fixed‐time artificial insemination in beef heifers following synchronisation of oestrous cycles using the CoSynch‐56 protocol

Gao

et al. 2021

Aust Veterinary J

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“…To investigate whether melatonin can alleviate this effect, bovine oocytes were treated with 20 mM NAM for 22 h in the presence and absence of 10 −7 M melatonin. The concentration of melatonin was selected based on previous studies on the protective role of melatonin during the IVM of bovine [33,34], porcine [35], and goat [36] oocytes. Initial inspection of oocyte maturation revealed that NAM at 20 mM concentration significantly reduced polar body extrusion (43:33 ± 1:67%) compared to the untreated control (66:67 ± 1:67%), whereas melatonin supplementation succeeded to restore the normal maturation (60:00 ± 5:00%) (Figures 1(a) and 1(b)).…”

Section: Melatonin Reduces Nam-associated Impairment Ofmentioning

confidence: 99%

Melatonin Alleviates the Toxicity of High Nicotinamide Concentrations in Oocytes: Potential Interaction with Nicotinamide Methylation Signaling

Sheikh

Mesalam

Song

et al. 2021

Oxidative Medicine and Cellular Longevity

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Despite the numerous studies on melatonin and nicotinamide (NAM, the active form of vitamin B3), the linkage between these two biomolecules in the context of signaling pathways regulating preimplantation embryo development has not yet been investigated. In this study, we used bovine oocyte model to elucidate the effect of melatonin on the developmental competence of oocytes under the stress of high NAM concentrations. Results showed that NAM (20 mM) administration during in vitro maturation (IVM) significantly reduced oocyte maturation and actin distribution, while induced reactive oxygen species (ROS) accumulation and mitochondrial dysfunction, the multiple deleterious effects that were alleviated by melatonin (10−7 M). The RT-qPCR and/or immunofluorescence showed upregulation of the apoptosis (Caspase-3, Caspase-9, and BAX), autophagy (Beclin-1, LC3A, LC3B, ATG7, LAMP1, and LAMP2), cell cycle (P21, P27, and P53), and DNA damage (COX2 and 8-OxoG) specific markers in oocytes matured under NAM treatment, compared to NAM-melatonin dual-treated and the untreated ones. In addition, the total cleavage and blastocyst development rate, as well as the total number of cells and the inner cell mass (ICM) per blastocyst, were reduced, while DNA fragmentation was induced, in the group of NAM sole treatment than NAM-melatonin cotreatment and control. Inspecting the underlying mechanisms behind NAM-associated toxicity revealed an increase in transcription pattern of NAM methylation (NNMT and AHCY) genes in NAM-treated oocytes while the opposite profile was observed upon melatonin supplementation. In conclusion, to our knowledge, this is the first study reporting that melatonin can protect oocytes and embryos from NAM-induced injury through its ROS-scavenging activity together with potential interaction with NAM methylation signaling.

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“…Exposing bovine oocytes to physiological relevant heat shock disrupts the nuclear and cytoplasmic maturation of the oocyte (3,4), impairs the oocyte's mitochondrial functioning, alters the transcriptional expression in the developing embryos (2), and increases the proportion of oocytes that undergo apoptosis (3). In a previous study we reported that exposing oocytes to heat shock (41.5°C) during maturation further impairs the cleavage timing of the developed embryo (5). Nevertheless, the effect of the season on embryo morphokinetics has never been completely characterized for bovine embryos.…”

Section: Introductionmentioning

confidence: 99%

Seasonal variation in the morphokinetics of in-vitro-derived bovine embryos is associated with the blastocyst developmental competence and gene expression

Yaacobi-Artzi

Kalo

Roth

2022

Front. Reprod. Health

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Summer heat stress is a major cause of reduced development of preimplantation embryos. Nevertheless, seasonal effects on embryo morphokinetics have been less studied. We used a non-invasive time-lapse system that allows continuous monitoring of embryos to study the seasonal impact on embryo morphokinetics. The experiments were performed during the cold and the hot seasons. Cumulus-oocyte complexes were aspirated from ovaries, in-vitro-matured, and fertilized. Putative zygotes were cultured in an incubator equipped with a time-lapse system. The cleavage and blastocyst formation rates were lower in the hot vs. the cold season (p < 0.01). The kinetics of the embryos differed between seasons, reflected by a delay in the second cleavage in the hot vs. the cold season (p < 0.03). The distribution of the embryos into different morphological grades (good, fair, and poor) throughout the first three cleavages differed between seasons, with a higher proportion of good-grade embryos in the hot season (p < 0.03). Cleaved embryos were categorized as either normal or abnormal, based on their first cleavage pattern. Normal cleavage was defined as when the first cleavage resulted in two equal blastomeres and further classified as either synchronous or asynchronous, according to their subsequent cleavages. Abnormal cleavage was defined as when the embryo directly cleaved into more than two blastomeres, it cleaved unequally into two unevenly sized blastomeres, or when the fusion of already divided blastomeres occurred. The proportion of abnormally cleaved embryos was higher in the hot season vs. the cold one (p < 0.01), reflected by a higher proportion of unequally cleaved embryos (p < 0.02). In the cold season, abnormally cleaved embryos had a lower potential to develop into blastocysts relative to their normally cleaved counterparts (p < 0.001). Blastocysts that developed in the cold and the hot seasons differed in the expression of genes that related to the cell cycle (STAT1; p < 0.01), stress (HSF1; p < 0.03), and embryo development (ZP3; p < 0.05). A higher expression level was recorded for the STAT1 and UHRF1 genes in blastocysts that developed from unequally vs. the synchronously cleaved embryos (p < 0.04). We provide the first evidence for a seasonal effect on embryo morphokinetics, which might explain the reduced embryo development during the hot season.

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Melatonin slightly alleviates the effect of heat shock on bovine oocytes and resulting blastocysts

Cited by 17 publications

References 63 publications

Two melatonin treatments improve the conception rate after fixed‐time artificial insemination in beef heifers following synchronisation of oestrous cycles using the CoSynch‐56 protocol

Two melatonin treatments improve the conception rate after fixed‐time artificial insemination in beef heifers following synchronisation of oestrous cycles using the CoSynch‐56 protocol

Melatonin Alleviates the Toxicity of High Nicotinamide Concentrations in Oocytes: Potential Interaction with Nicotinamide Methylation Signaling

Seasonal variation in the morphokinetics of in-vitro-derived bovine embryos is associated with the blastocyst developmental competence and gene expression

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