Melatonin reshapes the mitochondrial network and promotes intercellular mitochondrial transfer via tunneling nanotubes after ischemic‐like injury in hippocampal HT22 cells

Nasoni, Maria Gemma; Carloni, Silvia; Canonico, Barbara; Burattini, Sabrina; Cesarini, Erica; Papa, Stefano; Pagliarini, Marica; Ambrogini, Patrizia; Balduini, Walter; Luchetti, Francesca

doi:10.1111/jpi.12747

Cited by 66 publications

(57 citation statements)

References 64 publications

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“…Finally, damaged brain cells, including neurons and astrocytes, were reported to communicate with each other through TNTs. Whether this phenomenon is beneficial or detrimental in the context of ischemic brain repair is not yet known [ 66 , 133 , 134 ].…”

Section: Focus On An Organ: the Brain Role Of Intercellular Communica...mentioning

confidence: 99%

Intercellular Communication in the Brain through Tunneling Nanotubes

Khattar

Safi

Rodriguez

et al. 2022

Cancers

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Intercellular communication is essential for tissue homeostasis and function. Understanding how cells interact with each other is paramount, as crosstalk between cells is often dysregulated in diseases and can contribute to their progression. Cells communicate with each other through several modalities, including paracrine secretion and specialized structures ensuring physical contact between them. Among these intercellular specialized structures, tunneling nanotubes (TNTs) are now recognized as a means of cell-to-cell communication through the exchange of cellular cargo, controlled by a variety of biological triggers, as described here. Intercellular communication is fundamental to brain function. It allows the dialogue between the many cells, including neurons, astrocytes, oligodendrocytes, glial cells, microglia, necessary for the proper development and function of the brain. We highlight here the role of TNTs in connecting these cells, for the physiological functioning of the brain and in pathologies such as stroke, neurodegenerative diseases, and gliomas. Understanding these processes could pave the way for future therapies.

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Section: Focus On An Organ: the Brain Role Of Intercellular Communica...mentioning

confidence: 99%

Intercellular Communication in the Brain through Tunneling Nanotubes

Khattar

Safi

Rodriguez

et al. 2022

Cancers

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“…The natural exchange of different materials from one cell to another is an evolutionary defensive strategy to reduce the risk of cell death: on one hand, a healthy cell could share its organelles with a diseased one to promote damage repair [45,46] or improve cell respiration by the transfer of mitochondria in case of hypoxia [47]; on the other hand, this can also be used by cells in an attempt to dilute stressful inputs, leading to an increased number of stressed cells but lower stress levels. However, these mechanisms are also exploited and enhanced by numerous viruses, such as HIV [48], herpesviruses [49,50], influenza viruses [51,52], and more recently, SARS-CoV-2 [51,53,54].…”

Section: Tunneling Nanotubes 1what Are Tunneling Nanotubes?mentioning

confidence: 99%

“…It has been abundantly demonstrated in the literature that the formation rate of TNTs in vitro can be increased by several inputs linked to the culture protocol. This includes variables such as low levels of oxygen or a high presence of CO 2 , acidic pH, serum starvation, or low glucose concentrations [5,29,42,45]. All these conditions represent situations of cellular stress in which cells tend to connect in order to improve their survival, as previously described.…”

Section: Exogenous Modulation Of Tunneling Nanotubesmentioning

confidence: 99%

Tunneling Nanotubes: A New Target for Nanomedicine?

Ottonelli

Caraffi

Tosi

et al. 2022

IJMS

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Tunneling nanotubes (TNTs), discovered in 2004, are thin, long protrusions between cells utilized for intercellular transfer and communication. These newly discovered structures have been demonstrated to play a crucial role in homeostasis, but also in the spreading of diseases, infections, and metastases. Gaining much interest in the medical research field, TNTs have been shown to transport nanomedicines (NMeds) between cells. NMeds have been studied thanks to their advantageous features in terms of reduced toxicity of drugs, enhanced solubility, protection of the payload, prolonged release, and more interestingly, cell-targeted delivery. Nevertheless, their transfer between cells via TNTs makes their true fate unknown. If better understood, TNTs could help control NMed delivery. In fact, TNTs can represent the possibility both to improve the biodistribution of NMeds throughout a diseased tissue by increasing their formation, or to minimize their formation to block the transfer of dangerous material. To date, few studies have investigated the interaction between NMeds and TNTs. In this work, we will explain what TNTs are and how they form and then review what has been published regarding their potential use in nanomedicine research. We will highlight possible future approaches to better exploit TNT intercellular communication in the field of nanomedicine.

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“…The cell model of oxygen-glucose deprivation/ reoxygenation(OGD/R) injury was established followed as described before [19]. Brie y, after cell adherence, PC12 was washed with PBS 3 times and changed with EBSS at hypoxia incubator chamber (95% N 2 , 5% CO 2 ) for 6 h. 1 μg/mL GSH, GSH-AuNPs, and GSH-AuNCs was added with 10% DMEM and incubated in the incubator (5% CO 2 , 21% O 2 ) for 16 h. Immunohistochemistry staining PC12 cells and brain tissue slices (30 μm) were washed three times with 1× PBS for 5 min each, xed with 4% Paraformaldehyde (PFA) for 30 min.…”

Section: Oxygen Glucose Deprivation Model In Vitromentioning

confidence: 99%

Healing of ROS-induced cerebral ischemia/reperfusion injury by sustainable drug “chiral gold nanoclusters”

Zhuang

Shen

et al. 2022

Preprint

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Background Excessive reactive oxygen species (ROS) is produced by Ischemia-reperfusion (IR) injury that results in serious neurological disruption. Even though many antioxidant drugs can scavenge ROS, there has not been a sustainable drug for brain IR injury due to the limitation of blood-brain barrier (BBB) penetration, insufficient drug efficiency, unexpected side effects. Nanomaterials can improve these problems by delivering organic drugs, but the release of environmental hazards is still a concern. Results We used a simple one-pot synthesis method for the fabrication of IR-targeted nanomedicine, i.e., D-glutathione stabilized gold nanoclusters (GSH-AuNCs) for the treatment of brain IR injury. Though no additional drug was delivered, GSH-AuNCs themselves were found to penetrate BBB, suppressing ROS and neuroinflammation. GSH-AuNCs also exhibited excellent renal clearance efficiency due to the ultra-small sizes. This drug neither remains in the body nor pollutes the environment. Conclusion With high clearance ability, insignificant environmental concerns, GSH-AuNCs are promising for treating IR injury and can be used as a sustainable drug for practical applications.

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Melatonin reshapes the mitochondrial network and promotes intercellular mitochondrial transfer via tunneling nanotubes after ischemic‐like injury in hippocampal HT22 cells

Abstract: This is an open access article under the terms of the Creat ive Commo ns Attri bution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 66 publications

References 64 publications

Intercellular Communication in the Brain through Tunneling Nanotubes

Intercellular Communication in the Brain through Tunneling Nanotubes

Tunneling Nanotubes: A New Target for Nanomedicine?

Healing of ROS-induced cerebral ischemia/reperfusion injury by sustainable drug “chiral gold nanoclusters”

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