Melatonin Regulates Lipid Metabolism in Porcine Cumulus–Oocyte Complexes via the Melatonin Receptor 2

Jin, Jun-Xue; Sun, Jing‐Tao; Jiang, Chaoqian; Cui, Hongdi; Bian, Yaoyao; Lee, Sanghoon; Zhang, Lianjin; Lee, Byeong Chun; Liu, Zhong Hua

doi:10.3390/antiox11040687

Cited by 7 publications

(5 citation statements)

References 55 publications

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“…Interestingly, the expression of mtDNA copy number ( ND1 ) and mitochondrial biogenesis-related genes ( TFAM , NRF1/2 , TFB1M/2M and PGC1α ) were markedly increased by 10 −11 M ramelteon treatment. As the previous study, we investigated that melatonin regulated lipid homeostasis in porcine COCs via MT2 [ 12 ], whereas, porcine oocytes are only expressed the receptor MT2 [ 34 , 50 ]. Taken together, our results demonstrated that ramelteon reduces oxidative stress by maintenance of lipid homeostasis in porcine oocytes, which might be regulated via MT2.…”

Section: Discussionmentioning

confidence: 99%

“…Briefly, the cumulus expansion index (CEI) was performed as described by Jin et al [ 12 ]. After 42 h IVM, CEI was distinguished into five grades.…”

Section: Methodsmentioning

confidence: 99%

“…Moreover, Aizawa et al demonstrated that maintenance of LDs is essential for mouse preimplantation embryo development [ 11 ]. Therefore, the balance between lipogenesis and lipolysis might interact with the redox signaling during oocyte maturation [ 12 , 13 ]. In addition, there is 161 ± 18 ng of endogenous lipids in a porcine oocyte, which is much higher than other species [ 14 ]; therefore, it is an ideal models for studying lipid and fatty acid metabolism in female gametes [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

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Ramelteon Reduces Oxidative Stress by Maintenance of Lipid Homeostasis in Porcine Oocytes

et al. 2022

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This study aimed to determine the underlying mechanism of ramelteon on the competence of oocyte and subsequent embryo development in pigs during in vitro maturation (IVM). Our results showed that the cumulus expansion index was significantly lower in the control group compared to the ramelteon groups (p < 0.05). Moreover, supplementation of 10−11 and 10−9 M ramelteon significantly increased the cumulus expansion and development-related genes expression, and reduced apoptosis in cumulus cells (p < 0.05). In oocytes, the nuclear maturation rate was significantly improved in 10−11, 10−9, and 10−7 M ramelteon groups compared to the control (p < 0.05). Additionally, the level of intracellular GSH was significantly increased and ROS was significantly decreased in ramelteon-supplemented groups, and the gene expression of oocyte development and apoptosis were significantly up- and down-regulated by 10−11 and 10−9 M ramelteon (p < 0.05), respectively. The immunofluorescence results showed that the protein levels of GDF9, BMP15, SOD1, CDK1, and PGC1α were significantly increased by 10−11 M ramelteon compared to the control (p < 0.05). Although there was no significant difference in cleavage rate, the blastocyst formation rate, total cell numbers, and hatching/-ed rate were significantly improved in 10−11 M ramelteon group compared to the control (p < 0.05). Furthermore, embryo development, hatching, and mitochondrial biogenesis-related genes were dramatically up-regulated by 10−11 M ramelteon (p < 0.05). In addition, the activities of lipogenesis and lipolysis in oocytes were dramatically increased by 10−11 M ramelteon compared to the control (p < 0.05). In conclusion, supplementation of 10−11 M ramelteon during IVM improved the oocyte maturation and subsequent embryo development by reducing oxidative stress and maintenance of lipid homeostasis.

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Section: Discussionmentioning

confidence: 99%

“…Briefly, the cumulus expansion index (CEI) was performed as described by Jin et al [ 12 ]. After 42 h IVM, CEI was distinguished into five grades.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ramelteon Reduces Oxidative Stress by Maintenance of Lipid Homeostasis in Porcine Oocytes

et al. 2022

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“…Melatonin receptors (MT1 and 2), which couples with inhibitor G-protein (Gi), are identified in oocytes and granulosa cells ( 27, 28 ). The activated Gi inhibits the activation of adenylyl cyclases (ADCYs), resulting in decrease of cAMP (cyclic adenosine monophosphate), which regulates the activation of protein kinase A (PKA) and CREB (cAMP response element-binding protein) ( 29 ).…”

Section: Resultsmentioning

confidence: 99%

Maternal obesity may disrupt offspring metabolism by inducing oocyte genome hyper-methylation via increased DNMTs

Chao,

Lu,

et al. 2024

Preprint

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Maternal obesity has deleterious effects on oocyte genome methylation establishment, yet the underlying mechanisms remain unclear. In the present study, we first find that maternal obesity induced by high-fat diet (HFD) disturbs genomic methylation in oocytes, and at least a part of the altered methylation is transmitted to F2 oocytes and livers via females. We further identified that altered metabolites such as methionine and melatonin may play a key role in the re-methylation establishment in oocytes of obese mice. Exogenous melatonin treatment significantly reduces the hyper-methylation of HFD oocytes. The higher expression of DNMT3a and DNMT1 in HFD oocytes is also decreased by melatonin supplement, which may be mediated by cAMP/PKA/CREB pathway. These results suggest that maternal obesity-induced genomic methylation alterations in oocytes, can be partly transmitted to F2 in females, and that melatonin is involved in regulating the hyper-methylation of HFD oocytes via increasing the expression of DNMTs mediated by cAMP/PKA/CREB pathway.

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“…The maturation process of mammalian oocytes is an extremely complicated biological event that includes both nuclear and cytoplasmic changes [ 1 , 2 ]. Nuclear maturation mainly involves chromosome segregation, which has been well-studied [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Tannin Reduces the Incidence of Polyspermic Penetration in Porcine Oocytes

et al. 2022

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Tannin (TA) improves porcine oocyte cytoplasmic maturation and subsequent embryonic development after in vitro fertilization (IVF). However, the mechanism through which TA blocks polyspermy after IVF remains unclear. Hence, the biological function of organelles (cortical granule [CG], Golgi apparatus, endoplasmic reticulum [ER], and mitochondria) and the incidence of polyspermic penetration were examined. We found no significant difference in oocyte nuclear maturation among the 1 µg/mL, 10 µg/mL TA, and control groups. Moreover, 100 μg/mL TA significantly reduced 1st polar body formation rate compared to the other groups. Additionally, 1 and 10 μg/mL TA significantly increased the protein levels of GDF9, BMP15, and CDK1 compared to the control and 100 μg/mL TA groups. Interestingly, 1 and 10 μg/mL TA improved the normal distribution of CGs, Golgi, ER, and mitochondria by upregulating organelle-related gene expression and downregulating ER stress (CHOP) gene expression. Simultaneously, 1 and 10 μg/mL TA significantly increased the proportion of normal fertilized oocytes (2 pronuclei; 2 PN) and blastocyst formation rate compared to the control, as well as that of 100 μg/mL TA after IVF by upregulating polyspermy-related genes. In conclusion, TA during IVM enhances 2PN and blastocyst formation rates by regulating organelles’ functions and activities.

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Melatonin Regulates Lipid Metabolism in Porcine Cumulus–Oocyte Complexes via the Melatonin Receptor 2

Cited by 7 publications

References 55 publications

Ramelteon Reduces Oxidative Stress by Maintenance of Lipid Homeostasis in Porcine Oocytes

Ramelteon Reduces Oxidative Stress by Maintenance of Lipid Homeostasis in Porcine Oocytes

Maternal obesity may disrupt offspring metabolism by inducing oocyte genome hyper-methylation via increased DNMTs

Tannin Reduces the Incidence of Polyspermic Penetration in Porcine Oocytes

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