Melatonin regulates human bone sialoprotein gene transcription

Matsumura, Hideki; Ogata, Yorimasa

doi:10.2334/josnusd.56.67

Cited by 5 publications

(3 citation statements)

References 50 publications

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“…More promising result was obtained when focused on bone sialoprotein (BSP), a mineralized connective tissuespecific protein expressed in the early stage of cementum and bone mineralisation where melatonin induce BSP transcription via the CRE1 and CRE2 elements in the human BSP gene promoter [148].…”

Section: Melatonin In Bone Diseasementioning

confidence: 98%

An insight into the scientific background and future perspectives for the potential uses of melatonin

Anwar

Muhammad²,

Bader³

et al. 2015

Egyptian Journal of Basic and Applied Sciences

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Antiepileptic Antiosteoporotic Antioxidant Cardiovascular disease Circardian rhythm Hypnotic Immunodulatory Melatonin a b s t r a c t Melatonin is one of the most versatile and ubiquitous molecule widely distributed in nature has been reported to play a role in a wide variety of physiological responses including reproduction, circadian homeostasis, sleep, retinal neuromodulation, and vasomotor responses. In most vertebrates, including humans, melatonin is synthesized primarily in the pineal gland and is regulated by the environmental light ⁄ dark cycle via the suprachiasmatic nucleus. Melatonin is synthesized in all areas of the body such as gastrointestinal tract, skin, bone marrow, retina and in lymphocytes, from which it may influence other physiological functions through paracrine signalling. In addition to regulation of circadian rhythm of melatonin a variety of other physiological effects such as hypnotic, antidepressant, antiepileptic, oncostatic, immunomodulatory, antiosteoporotic, in cardiovascular disease, neuromodulatory and cerebral ischaemic condition have been reported.Moreover there is scarcity of literature that reviewed the scientific evidence for its use in these conditions. Therefore in this article we review recent advances in this research field, which is preceded by a concise account of general information about melatonin, melatonin receptors and intracellular signalling pathways for melatonin actions.

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Section: Melatonin In Bone Diseasementioning

confidence: 98%

An insight into the scientific background and future perspectives for the potential uses of melatonin

Anwar

Muhammad²,

Bader³

et al. 2015

Egyptian Journal of Basic and Applied Sciences

View full text Add to dashboard Cite

show abstract

“…Bone sialoprotein (BSP) is a mineralized connective tissue-specific protein that is expressed in the early stage of bone mineralization. Matsumura et al demonstrated that melatonin increases the transcription of BSP via activation of the cAMP response element (CRE) 1 and CRE2 to bind to the BSP gene promoter [ 102 ]. In addition, melatonin stimulates bone cell proliferation and increases bone mass by promoting osteoblast differentiation and the synthesis of type I collagen in humans.…”

Section: Advances In Antiosteoporosis Drugs and Biomaterialsmentioning

confidence: 99%

Insights into the Role of Macrophage Polarization in the Pathogenesis of Osteoporosis

Wang

Liu

et al. 2022

Oxidative Medicine and Cellular Longevity

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Millions of people worldwide suffer from osteoporosis, which causes bone fragility and increases the risk of fractures. Osteoporosis is closely related to the inhibition of osteogenesis and the enhancement of osteoclastogenesis. In addition, chronic inflammation and macrophage polarization may contribute to osteoporosis as well. Macrophages, crucial to inflammatory responses, display different phenotypes under the control of microenvironment. There are two major phenotypes, classically activated macrophages (M1) and alternatively activated macrophages (M2). Generally, M1 macrophages mainly lead to bone resorption, while M2 macrophages result in osteogenesis. M1/M2 ratio reflects the “fluid” state of macrophage polarization, and the imbalance of M1/M2 ratio may cause disease such as osteoporosis. Additionally, antioxidant drugs, such as melatonin, are applied to change the state of macrophage polarization and to treat osteoporosis. In this review, we introduce the mechanisms of macrophage polarization-mediated bone resorption and bone formation and the contribution to the clinical strategies of osteoporosis treatment.

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“…Melatonin positively regulates Runx2 in many cells and tissues, such as osteogenic and chondrogenic differentiation of human MSCs, osteoblastic differentiation of MSCs from rats, co‐culture models (transwell or layered) of human MSCs and peripheral blood monocytes, human osteosarcoma‐derived Saos2 cells, human periodontal ligament cells and cementoblasts, osteogenic potential of platelet‐rich plasma in dental stem‐cell cultures, the differentiation of mouse osteoblastic MC3T3‐E1 cells, primary bone marrow MSCs from ovariectomized mice, bovine ovarian granulosa cells, a blind mouse model (MMTV‐Neu transgenic mice), and pinealectomized or ovariectomized rats. Our study further confirmed that there was no direct interaction between melatonin and Runx2 although melatonin directly penetrates cytomembrane and enters the cell nucleus where it could bind to Runx2.…”

Section: Discussionmentioning

confidence: 99%

Identification of a novel melatonin‐binding nuclear receptor: Vitamin D receptor

Fang

Sun

et al. 2019

Journal of Pineal Research

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Previous studies confirmed that melatonin regulates Runx2 expression but the mechanism is unclear. There is a direct interaction between Runx2 and the vitamin D receptor (VDR). Herein, we observed a direct interaction between melatonin and the VDR but not Runx2 using isothermal titration calorimetry. Furthermore, this direct binding was detected only in the C-terminal ligand binding domain (LBD) of the VDR but not in the N-terminal DNA-binding domain (DBD) or the hinge region.Spectrophotometry indicated that melatonin and vitamin D3 (VD3) had similar uptake rates, but melatonin's uptake was significantly inhibited by VD3 until the concentration of melatonin was obviously higher than that of VD3 in a preosteoblastic cell line MC3T3-E1. GST pull-down and yeast two-hybrid assay showed that the interactive smallest fragments were on the 319-379 position of Runx2 and the N-terminus 110-amino acid DBD of the VDR. Electrophoretic mobility shift assay (EMSA) demonstrated that Runx2 facilitated the affinity between the VDR and its specific DNA substrate, which was further documented by a fluorescent EMSA assay where Cy3 labeled Runx2 co-localized with the VDR-DNA complex. Another fluorescent EMSA assay confirmed that the binding of the VDR to Runx2 was significantly enhanced with an increasing concentrations of the VDR, especially in the presence of melatonin; it was further documented using a co-immunoprecipitation assay that this direct interaction was markedly enhanced by melatonin treatment in the MC3T3-E1 cells. Thus, the VDR is a novel melatonin-binding nuclear receptor, and melatonin indirectly regulates Runx2 when it directly binds to the LBD and the DBD of the VDR, respectively. K E Y W O R D Sinteraction, melatonin, Runx2, vitamin D receptor, vitamin D3 2 of 14 | FANG et Al.

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Melatonin regulates human bone sialoprotein gene transcription

Cited by 5 publications

References 50 publications

An insight into the scientific background and future perspectives for the potential uses of melatonin

An insight into the scientific background and future perspectives for the potential uses of melatonin

Insights into the Role of Macrophage Polarization in the Pathogenesis of Osteoporosis

Identification of a novel melatonin‐binding nuclear receptor: Vitamin D receptor

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