Melatonin reduces ultraviolet-B induced cell damages and polyamine levels in human skin fibroblasts in culture

Lee, Kyu‐Suk; Lee, Won Suk; Suh, Seong-Il; Kim, Sang-Pyo; Lee, Sung-Ryong; Ryoo, Young Wook; Kim, Byung-Chun

doi:10.1038/emm.2003.35

Cited by 47 publications

(47 citation statements)

References 23 publications

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“…It was also noted that for UV-exposed fibroblasts, only 56% of the cells survived upon UV exposure (140 mJ/cm 2 ), while cells pre-incubated with 10−9 M melatonin caused a cell survival rate of 92.5% which was paralleled by significant reduction of lipid peroxidation and cell death [146]. In addition, comparative experiments using UV-treated fibroblasts showed a similar correlation in cell viability in presence of 10−7 M melatonin [147]. Finally, the most recent study suggested that melatonin at 10−3 M can protect NIH3T3 fibroblasts against UVA irradiation (15 J/cm 2 ) [148].…”

Section: Melatonin As a “Guardian” Of The Genome And Cellular And mentioning

confidence: 94%

Local Melatoninergic System as the Protector of Skin Integrity

Słomiński

Kleszczyński

Semak

et al. 2014

IJMS

131

169

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The human skin is not only a target for the protective actions of melatonin, but also a site of melatonin synthesis and metabolism, suggesting an important role for a local melatoninergic system in protection against ultraviolet radiation (UVR) induced damages. While melatonin exerts many effects on cell physiology and tissue homeostasis via membrane bound melatonin receptors, the strong protective effects of melatonin against the UVR-induced skin damage including DNA repair/protection seen at its high (pharmocological) concentrations indicate that these are mainly mediated through receptor-independent mechanisms or perhaps through activation of putative melatonin nuclear receptors. The destructive effects of the UVR are significantly counteracted or modulated by melatonin in the context of a complex intracutaneous melatoninergic anti-oxidative system with UVR-enhanced or UVR-independent melatonin metabolites. Therefore, endogenous intracutaneous melatonin production, together with topically-applied exogenous melatonin or metabolites would be expected to represent one of the most potent anti-oxidative defense systems against the UV-induced damage to the skin. In summary, we propose that melatonin can be exploited therapeutically as a protective agent or as a survival factor with anti-genotoxic properties or as a “guardian” of the genome and cellular integrity with clinical applications in UVR-induced pathology that includes carcinogenesis and skin aging.

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Section: Melatonin As a “Guardian” Of The Genome And Cellular And mentioning

confidence: 94%

Local Melatoninergic System as the Protector of Skin Integrity

Słomiński

Kleszczyński

Semak

et al. 2014

IJMS

131

169

View full text Add to dashboard Cite

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“…These observation have strong experimental support from in vitro studies. Thus, melatonin increases cell viability in UV-irradiated fibroblasts by counteracting the formation of polyamine levels ( 68 ), and the accumulation of malondialdehyde while decreasing apoptosis cells ( 69 ). Melatonin has also been shown to protect against UV light-induced damage in human leukocytes.…”

Section: Melatonin As Protector Against Skin Damagementioning

confidence: 99%

On the Role of Melatonin in Skin Physiology and Pathology

Słomiński

Fischer

Żmijewski

et al. 2005

ENDO

228

281

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Melatonin has been experimentally implicated in skin functions such as hair growth cycling, fur pigmentation, and melanoma control, and melatonin receptors are expressed in several skin cells including normal and malignant keratinocytes, melanocytes, and fibroblasts. Melatonin is also able to suppress ultraviolet (UV)-induced damage to skin cells and shows strong antioxidant activity in UV exposed cells. Moreover, we recently uncovered expression in the skin of the biochemical machinery involved in the sequential transformation of L-tryptophan to serotonin and melatonin. Existence of the biosynthetic pathway was confirmed by detection of the corresponding genes and proteins with actual demonstration of enzymatic activities for tryptophan hydroxylase, serotonin Nacetyl-transferase, and hydroxyindole-O-methyltransferase in extracts from skin and skin cells. Initial evidence for in vivo synthesis of melatonin and its metabolism was obtained in hamster skin organ culture and in one melanoma line. Therefore, we propose that melatonin (synthesized locally or delivered topically) could counteract or buffer external (environmental) or internal stresses to preserve the biological integrity of the organ and to maintain its homeostasis. Furthermore, melatonin could have a role in protection against solar radiation or even in the management of skin diseases.

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“…As previously described (Lee et al, 2003), UVB was supplied by a closely spaced array of seven Westinghouse FS-40 sunlamps, which delivered uniform irradiation at a distance of 38 cm. The energy output of UVB (290-320 nm) at 38 cm was measured with a UVB photometer (IL1350 photometer, International Light, Newburyport, MA).…”

Section: Uvb Irradiationmentioning

confidence: 99%