Melatonin receptor mRNA expression in human granulosa cells

Niles, Lennard P.; Wang, Jiaxu; Shen, Lei; Lobb, Derek K.; Younglai, Edward V.

doi:10.1016/s0303-7207(99)00135-5

Cited by 93 publications

(75 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This is consistent with the earlier reports on human and rat demonstrating the presence of MTNR1A and MTNR1B in granulosa cells and luteal cells (Soares et al 2003). The presence of high levels of melatonin in follicular fluid in human and binding sites in the ovary (Brzezinski et al 1987, Ronnberg et al 1990, Niles et al 1999 and, now, the presence of MTNR1A and MTNR1B in ovary suggest that melatonin directly acts as a modulator of ovarian functions.…”

Section: Discussionsupporting

confidence: 92%

Melatonin regulates delayed embryonic development in the short-nosed fruit bat, Cynopterus sphinx

Banerjee

Meenakumari²,

Udin³

et al. 2009

Reproduction

View full text Add to dashboard Cite

The aim of the present study was to evaluate the seasonal variation in serum melatonin levels and their relationship to the changes in the serum progesterone level, ovarian steroidogenesis, and embryonic development during two successive pregnancies of Cynopterus sphinx. Circulating melatonin concentrations showed two peaks; one coincided with the period of low progesterone synthesis and delayed embryonic development, whereas the second peak coincided with regressing corpus luteum. This finding suggests that increased serum melatonin level during November-December may be responsible for delayed embryonic development by suppressing progesterone synthesis. The study showed increased melatonin receptors (MTNR1A and MTNR1B) in the corpus luteum and in the utero-embryonic unit during the period of delayed embryonic development. The in vitro study showed that a high dose of melatonin suppressed progesterone synthesis, whereas a lower dose of melatonin increased progesterone synthesis by the ovary. The effects of melatonin on ovarian steroidogenesis are mediated through changes in the expression of peripheral-type benzodiazepine receptor, P450 side chain cleavage enzyme, and LH receptor proteins. This study further showed a suppressive impact of melatonin on the progesterone receptor (PGR) in the utero-embryonic unit; this effect might contribute to delayed embryonic development in C. sphinx. The results of the present study thus suggest that a high circulating melatonin level has a dual contribution in retarding embryonic development in C. sphinx by impairing progesterone synthesis as well as by inhibiting progesterone action by reducing expression of PGR in the utero-embryonic unit. Reproduction (2009) 138 935-944

show abstract

Section: Discussionsupporting

confidence: 92%

Melatonin regulates delayed embryonic development in the short-nosed fruit bat, Cynopterus sphinx

Banerjee

Meenakumari²,

Udin³

et al. 2009

Reproduction

View full text Add to dashboard Cite

show abstract

“…Using RT-PCR, mRNA for both types has been amplified from human fetal kidney (Drew et al, 1998), granulosa cells (Niles et al, 1999), PAZ6 adipocytes , and coronary arteries (Ekmekcioglu et al, 2001a). MT 1 melatonin receptors were localized by immunocytochemistry to both adult and fetal human kidney (Song et al, 1997;Drew et al, 1998).…”

Section: A Melatonin Receptor Expressionmentioning

confidence: 99%

“…Melatonin may also exert direct effects on ovarian function, because it is found in ovarian follicular fluid (Brzezinski et al, 1987;Rönnberg et al, 1990 (Niles et al, 1999;Clemens et al, 2001;Woo et al, 2001;Soares et al, 2003). Endogenous estrogens regulate the functional activity of melatonin receptors (Soares et al, 2003), whereas melatonin stimulates progesterone secretion from granulosa cells in several species including humans (Schaeffer and Sirotkin, 1995;Woo et al, 2001).…”

Section: B Melatonin Receptor Functionmentioning

confidence: 99%

International Union of Basic and Clinical Pharmacology. LXXV. Nomenclature, Classification, and Pharmacology of G Protein-Coupled Melatonin Receptors

et al. 2010

View full text Add to dashboard Cite

“…PCRs were carried out in separate reaction vessels in a volume of 100 μL with 0.5 mM primers, 1.5 mM MgCl 2 , 5 μL cDNA, 200 μM deoxyribonucleotide triphosphates, and 5 U Taq DNA polymerase. Products were examined on 1.5% agarose mini-gels at 120 V for 30 min and visualized under ultraviolet light (Niles et al, 1999).…”

Section: Cdna Clone Of Egfp Mrnamentioning

confidence: 99%

Transcriptional activity of an ovarian-specific promoter from rat in dairy goat granulosa cells

Liu

Yang²,

Cui³

et al. 2013

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Ovarian-specific promoter 1 (OSP-1) is a retrovirus-like element isolated from the complementary DNA library of rat that has been thought to be specifically expressed in ovary. To exploit this promoter in dairy goat ovary granulosa cells (GCs), OSP-1 from rat was used to construct the reporter vector pOSP-1-EGFP, in which egfp coding for enhanced green fluorescent protein (EGFP) was used as a reporter to examine the activity of OSP-1 in GCs. EGFP was successfully expressed in dairy goat GCs transfected with pOSP-1-EGFP. Reverse transcriptasepolymerase chain reaction analysis confirmed the tissue-specific transcription of EGFP messenger RNA in dairy goat GCs transfected with pOSP-1-EGFP. We concluded that OSP-1 promoter from rat can specifically drive foreign gene expression in dairy goat GCs. Thus, we obtained a tissue-specific regulation element and provided a potential tool for the research of regulation and development of the ovary in dairy goats.

show abstract

Melatonin receptor mRNA expression in human granulosa cells

Cited by 93 publications

References 21 publications

Melatonin regulates delayed embryonic development in the short-nosed fruit bat, Cynopterus sphinx

Melatonin regulates delayed embryonic development in the short-nosed fruit bat, Cynopterus sphinx

International Union of Basic and Clinical Pharmacology. LXXV. Nomenclature, Classification, and Pharmacology of G Protein-Coupled Melatonin Receptors

Transcriptional activity of an ovarian-specific promoter from rat in dairy goat granulosa cells

Contact Info

Product

Resources

About