Melatonin protects against rotenone‐induced oxidative stress in a hemiparkinsonian rat model

Saravanan, K.; Sindhu, Kizhakke M.; Mohanakumar, Kochupurackal P.

doi:10.1111/j.1600-079x.2006.00412.x

Cited by 112 publications

(68 citation statements)

References 67 publications

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“…Given that the nuclear Nrf2 level increased in the model livers (though without significant differences), the elevation of SOD activity is expected. This finding is consistent with a study [57] that investigated the neuroprotective effect of melatonin in a rotenone model of PD. It appears that Rg1 relieves liver injuries by up-regulating the activities of CAT, SOD, and GSH-Px to scavenge the free radicals induced by alcohol and CCl 4 .…”

Section: Discussionsupporting

confidence: 82%

Nrf2 pathway activation contributes to anti-fibrosis effects of ginsenoside Rg1 in a rat model of alcohol- and CCl4-induced hepatic fibrosis

Gao

Chu

et al. 2014

Acta Pharmacol Sin

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Aim: To investigate the anti-fibrosis effects of ginsenoside Rg1 on alcohol-and CCl 4 -induced hepatic fibrosis in rats and to explore the mechanisms of the effects. Methods: Rats were given 6% alcohol in water and injected with CCl 4 (2 mL/kg, sc) twice a week for 8 weeks. Rg1 (10, 20 and 40 mg/kg per day, po) was administered in the last 2 weeks. Hepatic fibrosis was determined by measuring serum biochemical parameters, HE staining, Masson's trichromic staining, and hydroxyproline and α-SMA immunohistochemical staining of liver tissues. The activities of antioxidant enzymes, lipid peroxidation, and Nrf2 signaling pathway-related proteins (Nrf2, Ho-1 and Nqo1) in liver tissues were analyzed. Cultured hepatic stellate cells (HSCs) of rats were prepared for in vitro studies. Results: In the alcohol-and CCl 4 -treated rats, Rg1 administration dose-dependently suppressed the marked increases of serum ALT, AST, LDH and ALP levels, inhibited liver inflammation and HSC activation and reduced liver fibrosis scores. Rg1 significantly increased the activities of antioxidant enzymes (SOD, GSH-Px and CAT) and reduced MDA levels in liver tissues. Furthermore, Rg1 significantly increased the expression and nuclear translocation of Nrf2 that regulated the expression of many antioxidant enzymes. Treatment of the cultured HSCs with Rg1 (1 μmol/L) induced Nrf2 translocation, and suppressed CCl 4 -induced cell proliferation, reversed CCl 4-induced changes in MDA, GPX, PCIII and HA contents in the supernatant fluid and α-SMA expression in the cells. Knockdown of Nrf2 gene diminished these actions of Rg1 in CCl 4 -treated HSCs in vitro. Conclusion: Rg1 exerts protective effects in a rat model of alcohol-and CCl 4 -induced hepatic fibrosis via promoting the nuclear translocation of Nrf2 and expression of antioxidant enzymes.

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Section: Discussionsupporting

confidence: 82%

Nrf2 pathway activation contributes to anti-fibrosis effects of ginsenoside Rg1 in a rat model of alcohol- and CCl4-induced hepatic fibrosis

Gao

Chu

et al. 2014

Acta Pharmacol Sin

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“…As a matter of fact, previous studies indicate that melatonin is neuroprotective in streptozotocin-induced rat model of diabetic neuropathy [104] . Once again, it would appear that the quenching of free radicals by melatonin might be the central mechanism for exerting neuroprotection [105,106] , although other melatonin-induced neuroprotective mechanisms cannot be ruled out. Thus, recent evidence in an experimental model of diabetic neuropathy suggests that melatonin modulates neuroinflammation by inhibiting the NF-κB pathway and downstream mediators of inflammation, and protects against oxidative stress by upregulating the nuclear erythroid 2-related factor 2 (Nrf2) pathway, thereby contributing to melatonin's neuroprotective effect [107] .…”

Section: Melatonin Modulates Diabetes-related Alterationsmentioning

confidence: 99%

Role of melatonin on diabetes-related metabolic disorders

Espino

Pariente²,

Rodríguez³

2011

WJD

100

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Melatonin is a circulating hormone that is mainly released from the pineal gland. It is best known as a regulator of seasonal and circadian rhythms, its levels being high during the night and low during the day. Interestingly, insulin levels are also adapted to day/night changes through melatonin-dependent synchronization. This regulation may be explained by the inhibiting action of melatonin on insulin release, which is transmitted through both the pertussis-toxin-sensitive membrane receptors MT1 and MT2 and the second messengers 3',5' -cyclic adenosine monophosphate, 3',5'-cyclic guanosine monophosphate and inositol 1,4,5-trisphosphate. Melatonin may influence diabetes and associated metabolic disturbances not only by regulating insulin secretion, but also by providing protection against reactive oxygen species, since pancreatic β-cells are very susceptible to oxidative stress because they possess only low-antioxidative capacity. On the other hand, in several genetic association studies, single nucleotide polymorphysms of the human MT2 receptor have been described as being causally linked to an elevated risk of developing type 2 diabetes. This suggests that these individuals may be more sensitive to the actions of melatonin, thereby leading to impaired insulin secretion. Therefore, blocking the melatonin-induced inhibition of insulin secretion may be a novel therapeutic avenue for type 2 diabetes.

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“…It has the main function of regulating circadian and seasonal rhythms as well as growth and morphogenesis. MLT also seems to possess antioxidant and free radical scavenging actions, which are independent from those mediated by receptors [18]. This antioxidant mechanism seems to be different from that of , classical' antioxidants such as vitamin E, vitamin C or glutathione, which are electron donors.…”

Section: Introductionmentioning

confidence: 98%

HPLC‐F analysis of melatonin and resveratrol isomers in wine using an SPE procedure

Mercolini

Saracino

Bugamelli

et al. 2008

J of Separation Science

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An original analytical method has been developed for the determination of the antioxidants trans-resveratrol (t-RSV) and cis-resveratrol (c-RSV) and of melatonin (MLT) in red and white wine. The method is based on HPLC coupled to fluorescence detection. Separation was obtained by using a RP column (C8, 150 mm x 4.6 mm id, 5 mum) and a mobile phase composed of 79% aqueous phosphate buffer at pH 3.0 and 21% ACN. Fluorescence intensity was monitored at lambda = 386 nm while exciting at lambda = 298 nm, mirtazapine was used as the internal standard. A careful pretreatment of wine samples was developed, using SPE with C18 cartridges (100 mg, 1 mL). The calibration curves were linear over the following concentration ranges: 0.03-5.00 ng/mL for MLT, 3-500 ng/mL for t-RSV and 1-150 ng/mL for c-RSV. The LOD values were 0.01 ng/mL for MLT, 1 ng/mL for t-RSV and 0.3 ng/mL for c-RSV. Precision data, as well as extraction yield and sample purification results, were satisfactory. Thus, the method seems to be suitable for the analysis of MLT and resveratrol isomers in wine samples. Moreover, wine total polyphenol content and antioxidant activity were evaluated.

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Melatonin protects against rotenone‐induced oxidative stress in a hemiparkinsonian rat model

Cited by 112 publications

References 67 publications

Nrf2 pathway activation contributes to anti-fibrosis effects of ginsenoside Rg1 in a rat model of alcohol- and CCl4-induced hepatic fibrosis

Nrf2 pathway activation contributes to anti-fibrosis effects of ginsenoside Rg1 in a rat model of alcohol- and CCl4-induced hepatic fibrosis

Role of melatonin on diabetes-related metabolic disorders

HPLC‐F analysis of melatonin and resveratrol isomers in wine using an SPE procedure

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