Melatonin promotes sleep-like state in zebrafish

Zhdanova, Irina V.; Wang, Steven Y.; Leclair, Ojingwa U.; Danilova, Nadia

doi:10.1016/s0006-8993(01)02444-1

Cited by 367 publications

(300 citation statements)

References 17 publications

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“…Ряд авторов [32][33][34][35] исследовали эффект седативных соединений на двигательную активность зебрафиш. Эти данные, суммированные в обзоре Barros и соавторов [36], свидетельствуют о применимости данной модели для тестирования действия лекарств на локомоторную активность (см.…”

Section: рисунокunclassified

Zebrafish as a model system for biomedical studies

et al. 2010

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Ключевые слова: зебрафиш (Danio rerio), разработка лекарств, острая токсичность, тератогенность, апоптоз, рак.ВВЕДЕНИЕ. Биомедицинские исследования, направленные на понимание патогенеза заболеваний человека на клеточном и молекулярном уровне и создание новых терапевтических средств, основаны на использование различных моделей животных. Зебрафиш (Danio rerio) -маленькая (размером до 4,5 см) пресноводная тропическая рыбка, обитающая в Индии и Южной Азии и хорошо известная любителям аквариумных рыб. Традиционно эту рыбку использовали в исследованиях в области молекулярной генетики и биологии развития, однако в последнее время она становится привлекательной в работах по созданию новых лекарственных препаратов и моделировании различных физиологических и патологических процессов [1][2][3][4][5][6].Эмбрион зебрафиш развивается быстро. Уже через три дня после оплодотворения у него функционирует сердце, кровеносная и нервная системы. Через четыре дня появляются личинки (мальки), способные есть и плавать. Эпителиальные клетки кишечника выделяют пищеварительные ферменты, гепатоциты секретируют желчь, панкреатические клетки продуцируют инсулин и карбоксипептидазы. Так как эмбрионы прозрачны, за их развитием можно наблюдать визуально с помощью микроскопа. Эмбрионы зебрафиш легко поглощают низкомолекулярные соединения из воды через кожу и жабры, а после 7 суток развития поглощение происходит через рот (а не через кожу) [7].Срок жизни полосатого Danio 3-5 лет. 120 * -адресат для переписки

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Section: рисунокunclassified

Zebrafish as a model system for biomedical studies

et al. 2010

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“…It may thus be involved in the regulation of states of "wakefulness" and energy homeostasis as in mammals. Larval zebrafish display a diurnal rhythm of locomotor activity and have periods of nocturnal immobility that are associated with a stereotypic posture and an increased arousal threshold, key behavioral indicators of a sleep-like state (22)(23)(24) …”

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“…Larval zebrafish display a diurnal rhythm of locomotor activity and have periods of nocturnal immobility that are associated with a stereotypic posture and an increased arousal threshold, key behavioral indicators of a sleep-like state (22)(23)(24). Sleep homeostatic mechanisms are also present; following rest dep-rivation, larvae display a compensatory rest rebound with an increased arousal threshold (22). Hypnotic compounds of the benzodiazepine and barbiturate families induce a sleep-like behavior in zebrafish; thus the role of GABA in behavioral sleep is likely to be functionally conserved (22).…”

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“…Sleep homeostatic mechanisms are also present; following rest dep-rivation, larvae display a compensatory rest rebound with an increased arousal threshold (22). Hypnotic compounds of the benzodiazepine and barbiturate families induce a sleep-like behavior in zebrafish; thus the role of GABA in behavioral sleep is likely to be functionally conserved (22). This may also be the case with other sleep-modulatory neurotransmitters that have been identified in the zebrafish (25,26) including acetylcholine, histamine, 5-hydroxytryptamine, and dopamine (27)(28)(29)(30)(31).…”

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confidence: 99%

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Regulation of Hypocretin (Orexin) Expression in Embryonic Zebrafish

Faraco

Appelbaum

Marin

et al. 2006

Journal of Biological Chemistry

105

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Hypocretins/orexins are neuropeptides involved in the regulation of sleep and energy balance in mammals. Conservation of gene sequence, hypothalamic localization of cell bodies, and projection patterns in adult zebrafish suggest that the architecture and function of the hypocretin system are conserved in fish. We report on the complete genomic structure of the zebrafish and Tetraodon hypocretin genes and the complete predicted hypocretin protein sequences from five teleosts. Using whole mount in situ hybridization, we have traced the development of hypocretin cells in zebrafish from onset of expression at 22 h post-fertilization through the first week of development. Promoter elements of similar size from zebrafish and Tetraodon were capable of driving efficient and specific expression of enhanced green fluorescent protein in developing zebrafish embryos, thus defining a minimal promoter region able to accurately mimic the native hypocretin pattern. This enhanced green fluorescent protein expression also revealed a complex pattern of projections within the hypothalamus, to the midbrain, and to the spinal cord. To further analyze the promoter, a series of deletion and substitution constructs were injected into embryos, and resulting promoter activity was monitored in the first week of development. A critical region of 250 base pairs was identified containing a core 13-base pair element essential for hypocretin expression.The hypocretin/orexin peptides (HCRT-1 and HCRT-2) 3 are neurotransmitters involved in the regulation of sleep and energy balance (1). HCRT-1 and HCRT-2 have significant homology and result from the cleavage of a common precursor encoded by the hcrt locus. In mammals, the hypocretin expressing cell clusters are located in the lateral hypothalamus and send widespread projections that innervate multiple brain areas and the spinal cord. The population is relatively small and involves ϳ70,000 neurons in the human brain (2). Projections are especially dense on several aminergic and cholinergic nuclei, most notably the adrenergic locus coeruleus and histaminergic tuberomammilary neurons (3).Loss of HCRT transmission causes the sleep disorder narcolepsy in humans, canines, and rodents (4 -6). The symptoms of narcolepsy in humans typically present in the second decade of life, usually in association with undetectable levels of HCRT-1 in the cerebrospinal fluid (7,8). Post-mortem studies indicate a 90 -95% loss of hypocretin-producing cells in human narcolepsy (2, 6, 9, 10). The process leading to cell death is unknown, but because of a strong association with human leukocyte antigen DQB1*0602 (11, 12), it is generally presumed that hypocretin cells are the target of an autoimmune process (13). This cell destruction in human narcolepsy is likely triggered by environmental factors interacting with a specific genetic background.An understanding of the processes underlying hcrt cell specification, differentiation, and maintenance as well as why they are susceptible to loss are key questions of both basic and...

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“…The photoperiod is the most important factor that entrains animal rhythms, including enzymatic secretion cycles, tied to the daily melatonin rhythms (Bromage et al 2001). Fish exhibit photoperiod-dependent circadian cycles of several processes, including locomotion (zebrafi sh; Zhdanova et al 2001), development (zebrafi sh; Danilova et al 2004), growth (rainbow trout; Falcón et al 2003), reproduction (salmon; Amano et al 2000Amano et al , 2004Senegal sole;Vera et al 2007), control and regulation of reproductive hormones and melatonin (European seabass; Bayarri et al 2004), and digestive enzyme activity (Yúfera et al 2012). Also, they have oscillators and circadian photo transduction drag mechanisms, suggesting that circadian pacemaker functions can be spread throughout the animal (zebrafi sh; Cahill 2002).…”

Section: Introductionmentioning

confidence: 99%