2021
DOI: 10.1186/s13287-021-02224-w
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Melatonin promotes bone marrow mesenchymal stem cell osteogenic differentiation and prevents osteoporosis development through modulating circ_0003865 that sponges miR-3653-3p

Abstract: Background Little is known about the implications of circRNAs in the effects of melatonin (MEL) on bone marrow mesenchymal stem cell (BMSC) osteogenic differentiation and osteoporosis (OP) progression. The aim of our study was to investigate circRNAs in MEL-regulated BMSC differentiation and OP progression. Methods BMSC osteogenic differentiation was measured by qRT-PCR, western blot (WB), Alizarin Red, and alkaline phosphatase (ALP) staining. Diff… Show more

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Cited by 51 publications
(44 citation statements)
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“…Wang et al 56 explored circRNAs expression profiling in the BMSCs after treated with melatonin or not by using RNA sequencing. They found that there were 209 circRNAs were differentially expressed in human BMSCs after treated with melatonin.…”
Section: Circrna E Xpre Ss Ion Profiling and Integ R Ative Analys Is In Scimentioning
confidence: 99%
See 1 more Smart Citation
“…Wang et al 56 explored circRNAs expression profiling in the BMSCs after treated with melatonin or not by using RNA sequencing. They found that there were 209 circRNAs were differentially expressed in human BMSCs after treated with melatonin.…”
Section: Circrna E Xpre Ss Ion Profiling and Integ R Ative Analys Is In Scimentioning
confidence: 99%
“…Wang et al 69 demonstrated that the circ_0003865 expression was downregulated in the BMSCs after treated with melatonin. Knock‐down of circ_0003865 promoted the expression of OPN, ALP and RUNX2 in BMSCs.…”
Section: Mechanisms Of Action Of Functionally Important Circrnas In Scimentioning
confidence: 99%
“…Interestingly, melatonin apparently promotes the osteogenic differentiation of BMMSCs by upregulating microRNA-92b-5p expression, which then enhances the differentiation of BMMSCs into mature osteoblasts by targeting intracellular adhesion molecule-1 (ICAM-1) [54]. In another study, melatonin promoted the osteogenic differentiation of BMMSCs and prevented the progression of osteoporosis by significantly decreasing the expression of circ_0003865, which subsequently increased levels of osteogenic marker genes (ALP, RUNX2 and OPN) and marked induction of osteogenic differentiation [59]. Moreover, melatonin-induced silencing of circ_0003865 increased the expression of miR-3653-3p, which substantially increased ALP, RUNX2, and OPN expression and promoted BMMSC osteogenic differentiation [59].…”
Section: Mc3t3-e1 Cells 1 µM Culturementioning
confidence: 96%
“…In another study, melatonin promoted the osteogenic differentiation of BMMSCs and prevented the progression of osteoporosis by significantly decreasing the expression of circ_0003865, which subsequently increased levels of osteogenic marker genes (ALP, RUNX2 and OPN) and marked induction of osteogenic differentiation [59]. Moreover, melatonin-induced silencing of circ_0003865 increased the expression of miR-3653-3p, which substantially increased ALP, RUNX2, and OPN expression and promoted BMMSC osteogenic differentiation [59].…”
Section: Mc3t3-e1 Cells 1 µM Culturementioning
confidence: 97%
“…Melatonin (Mlt), a neuroendocrine hormone primarily secreted by the pineal gland, exhibits a wide range of physiological functions, such as improving sleep and promoting poly-differentiation of stem cells, and is an antiaging, antioxidative, and antiosteoporotic agent ( Reiter et al, 2016 ; Nopparat et al, 2017 ; Gao et al, 2018 ; Sharma et al, 2018 ; Qiu et al, 2019 ; Wang et al, 2019 ; Wang et al, 2021 ). In recent years, the therapeutic effect of Mlt on tumors, including lung cancer, liver cancer, and breast cancer, has received great attention ( Ordonez et al, 2015 ; Borin et al, 2016 ; Ma et al, 2019 ) however, the effect of Mlt on GCTB remains unknown.…”
Section: Introductionmentioning
confidence: 99%