Melatonin preconditioning of bone marrow-derived mesenchymal stem cells promotes their engraftment and improves renal regeneration in a rat model of chronic kidney disease

Saberi, Kamran; Pasbakhsh, Parichehr; Omidi, Abdollah; Borhani-Haghighi, Maryam; Nekoonam, Saeid; Omidi, Negar; Ghasemi, Sodabeh; Kashani, Iraj Ragerdi

doi:10.1007/s10735-019-09812-4

Cited by 33 publications

(38 citation statements)

References 41 publications

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“…Nevertheless, whether melatonin stimulus acts in a paracrine fashion to exert its neuroprotective effect requires further investigation. It has been reported that melatonin reconditioning increases the expression of matrix metalloproteinase-9 (MMP-9) and MMP-13 and decreased the expression of TGF-β to restrain fibrosis (Saberi et al, 2019). In addition, melatonin-stimulated mesenchymal stem cell (MSC)derived exosomes enhance functional recovery in acute liver ischemia-reperfusion injury, indicating that melatonin alters the exosome content for modulation of the microenvironment by paracrine mechanisms (Yoon et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Melatonin Enhances the Therapeutic Effect of Plasma Exosomes Against Cerebral Ischemia-Induced Pyroptosis Through the TLR4/NF-κB Pathway

Wang

Zhang

et al. 2020

Front. Neurosci.

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Introduction: Ischemic stroke-induced inflammation and inflammasome-dependent pyroptotic neural death cause serious neurological injury. Nano-sized plasma exosomes have exhibited therapeutic potential against ischemia and reperfusion injury by ameliorating inflammation. To enhance its therapeutic potential in patients with ischemic injury, we isolated exosomes from melatonin-treated rat plasma and assessed the neurological protective effect in a rat model of focal cerebral ischemia. Methods: Basal plasma exosomes and melatonin-treated plasma exosomes were isolated and intravenously injected into a rat model of focal cerebral ischemia. Neurological recovery was evaluated by determining the modified neurological severity score (mNSS), infarct volume, and brain water content. Pyroptosis in the ischemic cortex was detected through dUTP nick-end labeling (TUNEL) assay, lactate dehydrogenase (LDH) release, and gasdermin D (GSDMD) cleavage. NLRP3 inflammasome assembly and global inflammatory cytokine secretion were detected by enzyme-linked immunosorbent assay (ELISA) and Western blot assay. In immunized Sprague-Dawley rats, microglia pyroptosis was determined through a positive percentage of IBA1 + and caspase-1 (p20) + cells. Finally, the microRNA (miRNA) profiles in melatonin-treated plasma exosomes were analyzed by exosome miRNA microarray analysis. Results: Melatonin treatment enhanced plasma exosome therapeutic effects against ischemia-induced inflammatory responses and inflammasome-mediated pyroptosis. In addition, we confirmed that ischemic stroke-induced pyroptotic cell death occurred in the microglia and neuron, while the administration of melatonin-treated exosomes further effectively decreased the infarct volume and improved recovery of function via regulation of the TLR4/NF-κB signaling pathway. Finally, the altered miRNA profiles in the melatonin-treated plasma exosomes demonstrated the regulatory mechanisms involved in neurological recovery after ischemic injury.

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Section: Discussionmentioning

confidence: 99%

Melatonin Enhances the Therapeutic Effect of Plasma Exosomes Against Cerebral Ischemia-Induced Pyroptosis Through the TLR4/NF-κB Pathway

Wang

Zhang

et al. 2020

Front. Neurosci.

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“…Homing and lodgment of MSCs may be tissue‐dependent as marrow MSCs have been found to home to hypopharyngeal cancers, 125 but they do not appear to home to primary prostate tumors, for example. Likewise, in chronic kidney disease, there is low level homing and poor survival of transplanted marrow‐derived MSCs 126 . In mice, marrow‐derived CXCR4‐overexpressing MSCs homed to the intestine and improved colitis in inflammatory bowel disease states 127 .…”

Section: Msc Homingmentioning

confidence: 99%

Stem cell homing: From physiology to therapeutics

2020

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Stem cell homing is a multistep endogenous physiologic process that is also used by exogenously administered hematopoietic stem and progenitor cells (HSPCs). This multistep process involves cell migration and is essential for hematopoietic stem cell transplantation. The process can be manipulated to enhance ultimate engraftment potential, and understanding stem cell homing is also important to the understanding of stem cell mobilization. Homing is also of potential importance in the recruitment of marrow mesenchymal stem and stromal cells (MSCs) to sites of injury and regeneration. This process is less understood but assumes importance when these cells are used for repair purposes. In this review, the process of HSPC and MSC homing is examined, as are methods to enhance this process.

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“…CD44-hyaluronic acid (CD44-HA) interactions are involved in the process of homing of MSCs to the damaged renal tissue and promote renal function repair following acute tubular injury and chronic renal failure [111]. Due to their vitality in enhancing the kidney-directional migration of transplanted MSCs for increasing the efficiency of tissue repair, some novel preconditioning strategies were explored, including incubation with cytokines or chemical compounds [112][113][114], co-injection [115,116], hypoxia stimulation [117], and genetic modifications [118,119]. Also, pulse-focused ultrasound (pFUS) has elicited local molecular responses through mechanotransduction, thus enhancing the renal homing of circulating MSCs [120][121][122].…”

Section: Mesenchymal Stem/stromal Cellsmentioning

confidence: 99%

Stem cells: a potential treatment option for kidney diseases

Cheng

Pan

et al. 2020

Stem Cell Res Ther

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The prevalence of kidney diseases is emerging as a public health problem. Stem cells (SCs), currently considered as a promising tool for therapeutic application, have aroused considerable interest and expectations. With self-renewal capabilities and great potential for proliferation and differentiation, stem cell therapy opens new avenues for the development of renal function and structural repair in kidney diseases. Mounting evidence suggests that stem cells exert a therapeutic effect mainly by replacing damaged tissues and paracrine pathways. The benefits of various types of SCs in acute kidney disease and chronic kidney disease have been demonstrated in preclinical studies, and preliminary results of clinical trials present its safety and tolerability. This review will focus on the stem cell-based therapy approaches for the treatment of kidney diseases, including various cell sources used, possible mechanisms involved, and outcomes that are generated so far, along with prospects and challenges in clinical application.

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Melatonin preconditioning of bone marrow-derived mesenchymal stem cells promotes their engraftment and improves renal regeneration in a rat model of chronic kidney disease

Cited by 33 publications

References 41 publications

Melatonin Enhances the Therapeutic Effect of Plasma Exosomes Against Cerebral Ischemia-Induced Pyroptosis Through the TLR4/NF-κB Pathway

Melatonin Enhances the Therapeutic Effect of Plasma Exosomes Against Cerebral Ischemia-Induced Pyroptosis Through the TLR4/NF-κB Pathway

Stem cell homing: From physiology to therapeutics

Stem cells: a potential treatment option for kidney diseases

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