Melatonin Nocturnal Surge Modulates Nicotinic Receptors and Nicotine-Induced [<sup>3</sup>H]Glutamate Release in Rat Cerebellum Slices

Markus, Regina Pekelmann; Santos, J. M.; Zago, Wagner; Renó, Lívia A. C.

doi:10.1124/jpet.102.045625

Cited by 28 publications

(29 citation statements)

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“…Both strategies resulted in the inhibition of the development of the greater α-bungarotoxin-sensitive response to acetylcholine in DIV5 cultures, while they had no effect on the smaller α-bungarotoxininsensitive response observed at DIV4. This result agrees with the functional responses observed in the rat vas deferens (13) and in the cerebellum (11), where it was shown that melatonin increases the functional response to stimulation of α-bungarotoxin-sensitive acetylcholine receptors.…”

Section: Pharmacological Characterization Of Functional Nachrs In Retsupporting

confidence: 92%

“…The data obtained with the embryo retinal cells in culture are somewhat similar to those observed in rat sympathetic nerve terminals and in the cerebellum (11,12). In all cases melatonin only interferes with nAChRs sensitive to α-bungarotoxin.…”

Section: Pharmacological Characterization Of Functional Nachrs In Retsupporting

confidence: 76%

“…Therefore, it was not a surprise that the reduction in the number of α-bungarotoxin binding sites was accompanied by the appearance of the functional response between DIV4 and DIV5 retinal cultures. Melatonin has been shown to modulate the expression and the activity of α7 nAChRs in sympathetic nerve terminals of rat vas deferens (10,12,13) and cerebellum (11). The release of noradrenaline in the vas deferens or of glutamate in the cerebellum is increased by melatonin.…”

Section: Pharmacological Characterization Of Functional Nachrs In Retmentioning

confidence: 99%

“…Acetylcholine-induced release of neurotransmitter from sympathetic nerve terminals of the vas deferens and cerebellum slices of the rat is higher in the dark phase than in the light phase of the day. In both cases the effect of acetylcholine is mediated by nAChRs sensitive to α-bungarotoxin (11,12). Melatonin, the hormone released by the pineal gland during the dark phase of the day, is responsible for the diurnal variation of nAChR-mediated responses.…”

Section: Introductionmentioning

confidence: 99%

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Influence of melatonin on the development of functional nicotinic acetylcholine receptors in cultured chick retinal cells

Sampaio

Hamassaki‐Britto

Markus

2005

Braz J Med Biol Res

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The influence of melatonin on the developmental pattern of functional nicotinic acetylcholine receptors was investigated in embryonic 8-day-old chick retinal cells in culture. The functional response to acetylcholine was measured in cultured retina cells by microphysiometry. The maximal functional response to acetylcholine increased 2.7 times between the 4th and 5th day in vitro (DIV4, DIV5), while the Bmax value for [ 125 I]-α-bungarotoxin was reduced. Despite the presence of α8-like immunoreactivity at DIV4, functional responses mediated by α-bungarotoxin-sensitive nicotinic acetylcholine receptors were observed only at DIV5. Mecamylamine (100 µM) was essentially without effect at DIV4 and DIV5, while dihydro-ß-erythroidine (10-100 µM) blocked the response to acetylcholine (3.0 nM-2.0 µM) only at DIV4, with no effect at DIV5. Inhibition of melatonin receptors with the antagonist luzindole, or melatonin synthesis by stimulation of D4 dopamine receptors blocked the appearance of the α-bungarotoxin-sensitive response at DIV5. Therefore, α-bungarotoxin-sensitive receptors were expressed in retinal cells as early as at DIV4, but they reacted to acetylcholine only after DIV5. The development of an α-bungarotoxin-sensitive response is dependent on the production of melatonin by the retinal culture. Melatonin, which is produced in a tonic manner by this culture, and is a key hormone in the temporal organization of vertebrates, also potentiates responses mediated by α-bungarotoxin-sensitive receptors in rat vas deferens and cerebellum. This common pattern of action on different cell models that express α-bungarotoxin-sensitive receptors probably reflects a more general mechanism of regulation of these receptors. Correspondence

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Section: Pharmacological Characterization Of Functional Nachrs In Retsupporting

confidence: 92%

Section: Pharmacological Characterization Of Functional Nachrs In Retsupporting

confidence: 76%

Section: Pharmacological Characterization Of Functional Nachrs In Retmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Influence of melatonin on the development of functional nicotinic acetylcholine receptors in cultured chick retinal cells

Sampaio

Hamassaki‐Britto

Markus

2005

Braz J Med Biol Res

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“…The subunits α3, α4, and β2 are located in granule cells, while deep cerebellar nuclei express α4 and α2 subunits and Purkinje cells express α4, α7, and β2 subunits (Nakayama 1997). In the cerebellum, α7 nAChRs induce the release of glutamate (Reno 2004;De Fillipi 2001), which varies throughout the course of the day under melatonin control (Markus et al 2003). …”

Section: Introductionmentioning

confidence: 97%

Poster Abstracts

Gozes

2009

J Mol Neurosci

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Melatonin administration alters nicotine preference consumption via signaling through high-affinity melatonin receptors

et al. 2015

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Rationale While it is known that tobacco use varies across the 24-hour day, the time-of-day effects are poorly understood. Findings from several previous studies indicate a potential role for melatonin in these time-of-day effects; however the specific underlying mechanisms have not been well characterized. Understanding of these mechanisms may lead to potential novel smoking cessation treatments. Objective Examine the role of melatonin and melatonin receptors in nicotine free choice consumption Methods A two-bottle oral nicotine choice paradigm was utilized with melatonin supplementation in melatonin deficient mice (C57BL/6J) or without melatonin supplementation in mice proficient at melatonin synthesis (C3H/Ibg) compared to melatonin proficient mice lacking both or one of the high affinity melatonin receptors (MT1 and MT2; double null mutant DM, or MT1 or MT2). Preference for bitter and sweet tastants also was assessed in wild type and MT1 and MT2 DM mice. Finally, home cage locomotor monitoring was performed to determine the effect of melatonin administration on activity patterns. Results Supplemental melatonin in drinking water significantly reduced free-choice nicotine consumption in C57BL/6J mice, which do not produce endogenous melatonin, while not altering activity patterns. Independently, genetic deletion of both MT1 and MT2 receptors in a melatonin proficient mouse strain (C3H) resulted in significantly more nicotine consumption than controls. However single genetic deletion of either the MT1 or MT2 receptor alone did not result in increased nicotine consumption. Deletion of MT1 and MT2 did not impact taste preference. Conclusions This study demonstrates that nicotine consumption can be affected by exogenous or endogenous melatonin and requires at least one of the high-affinity melatonin receptors. The fact that expression of either the MT1 or MT2 melatonin receptor is sufficient to maintain lower nicotine consumption suggests functional overlap and potential mechanistic explanations. Keywords: Melatonin, Nicotine, Mice, Preference Drinking

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Melatonin Nocturnal Surge Modulates Nicotinic Receptors and Nicotine-Induced [³H]Glutamate Release in Rat Cerebellum Slices

Cited by 28 publications

References 34 publications

Influence of melatonin on the development of functional nicotinic acetylcholine receptors in cultured chick retinal cells

Influence of melatonin on the development of functional nicotinic acetylcholine receptors in cultured chick retinal cells

Poster Abstracts

Melatonin administration alters nicotine preference consumption via signaling through high-affinity melatonin receptors

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Melatonin Nocturnal Surge Modulates Nicotinic Receptors and Nicotine-Induced [3H]Glutamate Release in Rat Cerebellum Slices

Cited by 28 publications

References 34 publications

Influence of melatonin on the development of functional nicotinic acetylcholine receptors in cultured chick retinal cells

Influence of melatonin on the development of functional nicotinic acetylcholine receptors in cultured chick retinal cells

Poster Abstracts

Melatonin administration alters nicotine preference consumption via signaling through high-affinity melatonin receptors

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Melatonin Nocturnal Surge Modulates Nicotinic Receptors and Nicotine-Induced [³H]Glutamate Release in Rat Cerebellum Slices