Melatonin maintains calcium-binding calretinin-positive neurons in the dentate gyrus during aging of Balb/C mice

Ramírez-Rodríguez, Gerardo; Gómez-Sánchez, Ariadna; Ortíz-López, Leonardo

doi:10.1016/j.exger.2014.10.014

Cited by 13 publications

(13 citation statements)

References 77 publications

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“…The therapy persisted either of daily intraperitoneal melatonin injections (8 mg/kg)—dissolved in 0.9% saline—(JL‐Mel) or of environmental enrichment as a lifestyle approach (JL‐ENR). We considered 8 mg/kg as appropriate as several studies reported that this melatonin dose has beneficial effects on adult hippocampal neurogenesis . Environmental enrichment consisted of an enlarged cage (0.74 m×0.3 m×0.74 m) containing a rearrangeable set of plastic tubes, colourful cartons, cardboard tubes, grids, varying shelters, and alternating food and drinking spots .…”

Section: Methodsmentioning

confidence: 99%

Melatonin restores hippocampal neural precursor cell proliferation and prevents cognitive deficits induced by jet lag simulation in adult mice

Iggena

Winter

Steiner

2017

Journal of Pineal Research

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Frequent flyers and shift workers undergo circadian dysrhythmia with adverse impact on body and mind. The circadian rhythm disorder "jet lag" disturbs hippocampal neurogenesis and spatial cognition, which represent morphological and functional adult brain plasticity. This raises the question if pro-neurogenic stimuli might prevent those consequences. However, suitable measures to mitigate jet lag-induced adverse effects on brain plasticity have been neglected so far. Here, we used adult C57Bl6 mice to investigate the pro-neurogenic stimuli melatonin (8 mg/kg i.p.) as well as environmental enrichment as potential measures. We applied photoperiod alterations to simulate "jet lag" by shortening the dark period every third day by 6 hours for 3 weeks. We found that "jet lag" simulation reduced hippocampal neural precursor cell proliferation by 24% and impaired spatial memory performance in the water maze indicated by a prolonged swim path to the target (~23%). While melatonin prevented both the cellular (~1%) as well as the cognitive deficits (~5%), environmental enrichment only preserved precursor cell proliferation (~12%). Our results indicate that lifestyle interventions are insufficient to completely compensate jet lag-induced consequences. Instead, melatonin is required to prevent cognitive impairment caused by the same environmental factors to which frequent flyers and shift workers are typically exposed to.

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Section: Methodsmentioning

confidence: 99%

Melatonin restores hippocampal neural precursor cell proliferation and prevents cognitive deficits induced by jet lag simulation in adult mice

Iggena

Winter

Steiner

2017

Journal of Pineal Research

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“…Golgi-Cox impregnation was revealed with 18.7% ammonia solution and fixed in Kodak rapid fix (Kodak, Rochester, NY, USA). Brain slices were dehydrated and clarified in Neoclear before they were coverslipped with Neomount medium (Merck, Whitehouse Station, NJ, USA) [ 35 , 36 ]. Twenty Golgi-stained neurons in the granule cell layer (GCL) of the dentate gyrus were analyzed to determine dendritic spine density in every 10 μm of secondary dendrites of rodents exposed to either control- or RVTL (40 mg/kg).…”

Section: Methodsmentioning

confidence: 99%

“…Hippocampi from control- or RVTL-treated mice were dissected and lysed as reported previously [ 35 , 36 ]. Control- and RVTL-treated mice were housed under normal conditions in groups of 4–5 animals.…”

Section: Methodsmentioning

confidence: 99%

Resveratrol Enhances Neuroplastic Changes, Including Hippocampal Neurogenesis, and Memory in Balb/C Mice at Six Months of Age

Torres-Pérez¹,

Tellez-Ballesteros²,

Ortíz-López³

et al. 2015

PLoS ONE

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Resveratrol (RVTL) is a flavonoid found in red wine and has been publicized heavily as an anti-aging compound. Indeed, basic research confirms that although there is much hype in the promotion of RVTL, flavonoids such as RVTL have a wide range of biological effects. We here investigated the effects of RVTL treatment on hippocampal plasticity and memory performance in female Balb/C mice, a strain with low baseline levels of adult neurogenesis. Two weeks of treatment with RVTL (40 mg/kg) induced the production of new neurons in vivo by increasing cell survival and possibly precursor cell proliferation. In addition, RVTL decreased the number of apoptotic cells. The number of doublecortin (DCX)-expressing intermediate cells was increased. RVTL stimulated neuronal differentiation in vitro without effects on proliferation. In the dentate gyrus, RVTL promoted the formation and maturation of spines on granule cell dendrites. RVTL also improved performance in the step down passive avoidance test. The RVTL-treated mice showed increase in the levels of two key signaling proteins, phospho-Akt and phospho-PKC, suggesting the involvement of these signaling pathways. Our results support the vision that flavonoids such as resveratrol deserve further examination as plasticity-inducing compounds in the context of successful cognitive aging.

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“…The beneficial effects of melatonin on SGZ precursors may rely on its capacity to increase the levels of phospho-c-Raf and phosphoextracellular signal-regulated kinase 1/2 (ERK1/2) through melatonin receptor [77]. Other studies have reported neuroprotective effect of melatonin in different animal models, including irradiation, aging, spinal cord injury, ischemia, hypoxia, and pinealectomy [34–37, 54, 77–80]. …”

Section: Discussionmentioning

confidence: 99%

“…This hormone mediates its effects through the cell membrane receptors MT1 and MT2 [23, 24] and regulates antioxidant enzymes [25, 26] by switching on/off intracellular signaling cascades [27, 28], as well as by scavenging oxygen free radical [29–33]. In addition, melatonin promotes neurogenesis under diverse conditions, such as ovariectomy, pinealectomy, aging, or circadian disruption [17, 34–40]. In this study, we used a prophylactic administration of melatonin for 14 days (before and during 96 hours of REMSD) to determine if melatonin exerted a protective effect in neural precursor cells.…”

Section: Introductionmentioning

confidence: 99%

Prophylactic Role of Oral Melatonin Administration on Neurogenesis in Adult Balb/C Mice during REM Sleep Deprivation

López-Armas

Flores-Soto

Chaparro-Huerta

et al. 2016

Oxidative Medicine and Cellular Longevity

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Purpose. The aim of this study was to assess the effect of melatonin in the proliferation of neural progenitors, melatonin concentration, and antiapoptotic proteins in the hippocampus of adult mice exposed to 96 h REM sleep deprivation (REMSD) prophylactic administration of melatonin for 14 days. Material and Methods. Five groups of Balb/C mice were used: (1) control, (2) REMSD, (3) melatonin (10 mg/kg) plus REMSD, (4) melatonin and intraperitoneal luzindole (once a day at 5 mg/kg) plus REMSD, and (5) luzindole plus REMSD. To measure melatonin content in hippocampal tissue we used HPLC. Bcl-2 and Bcl-xL proteins were measured by Western Blot and neurogenesis was determined by injecting 5-bromo-2-deoxyuridine (BrdU) and BrdU/nestin expressing cells in the subgranular zone of the dentate gyrus were quantified by epifluorescence. Results. The melatonin-treated REMSD group showed an increased neural precursor in 44% with respect to the REMSD group and in 28% when contrasted with the control group (P < 0.021). The melatonin-treated REMSD group also showed the highest expression of Bcl-2 and Bcl-xL as compared to the rest of the groups. Conclusion. The exogenous administration of melatonin restores the tissue levels of sleep-deprived group and appears to be an efficient neuroprotective agent against the deleterious effects of REMSD.

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Melatonin maintains calcium-binding calretinin-positive neurons in the dentate gyrus during aging of Balb/C mice

Cited by 13 publications

References 77 publications

Melatonin restores hippocampal neural precursor cell proliferation and prevents cognitive deficits induced by jet lag simulation in adult mice

Melatonin restores hippocampal neural precursor cell proliferation and prevents cognitive deficits induced by jet lag simulation in adult mice

Resveratrol Enhances Neuroplastic Changes, Including Hippocampal Neurogenesis, and Memory in Balb/C Mice at Six Months of Age

Prophylactic Role of Oral Melatonin Administration on Neurogenesis in Adult Balb/C Mice during REM Sleep Deprivation

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