Melatonin inhibits osteosarcoma stem cells by suppressing SOX9-mediated signaling

Qu, Hao; Xue, Yue; Lian, Wenwen; He, Jiawei; Fu, Qihong; Zhong, Lijia; Lin, Nong; Lai, Lihua; Ye, Zhaoming; Wang, Qingqing

doi:10.1016/j.lfs.2018.04.030

Cited by 38 publications

(39 citation statements)

References 51 publications

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“…CSCs, a small subpopulation of cells within a tumor, are considered to possess the capacity to self‐renew and maintain the tumor phenotype, which may annul the clinical treatment . Here we investigated whether BD could inhibit sphere‐forming ability of osteosarcoma cells and self‐renewal capacity of OSCs using sphere‐forming assay . Osteosarcoma cells treated with BD formed much smaller and significantly fewer spheres (Figure A).…”

Section: Resultsmentioning

confidence: 99%

“…The advent of adjuvant therapies and advances in surgical techniques, have caused the overall survival rates of osteosarcoma patients to reach 60%‐75% . However, the 5‐year survival rate for those with metastasis remains less than 30% and is almost unchanged over the past three decades . Current chemo‐drugs are limited due to their side effects and development of resistance .…”

Section: Discussionmentioning

confidence: 99%

“…Sphere-forming assay was performed accordingly as described previously. 5,11 MNNG/HOS cells (2000 cells/well) were inoculated in six-well ultralow attachment plates (Corning Inc) in serum-free α-MEM containing 1% B27 Supplement (Invitrogen), 10 ng/mL human EGF (Pepro Tech), and 10 ng/mL human bFGF (Pepro Tech). After 1 week, spheres were observed and photographed through a microscope, and sphere quantity was determined manually.…”

Section: Sphere-forming Assaymentioning

confidence: 99%

“…Cancer stem cells (CSCs) are a small population of cells within the tumor that exhibit properties similar to normal stem cells but have tumor initiating ability in appropriate animal models . Some research findings suggest that CSCs are responsible for tumor initiation, self‐renewal, propagation, and metastasis . Since the CSC hypothesis was proposed, many studies have been conducted to identify osteosarcoma stem cells (OSCs) .…”

Section: Introductionmentioning

confidence: 99%

“…4,5 Some research findings suggest that CSCs are responsible for tumor initiation, self-renewal, propagation, and metastasis. [5][6][7][8] Since the CSC hypothesis was proposed, many studies have been conducted to identify osteosarcoma stem cells (OSCs). 7 OSCs are reported to contribute to tumor relapse, metastasis, and resistance to chemotherapy.…”

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confidence: 99%

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Bruceine D inhibits tumor growth and stem cell‐like traits of osteosarcoma through inhibition of STAT3 signaling pathway

Wang

Zhong

et al. 2019

Cancer Medicine

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Patients with osteosarcoma exhibiting resistance to chemotherapy or presenting with metastasis usually have a poor prognosis. Osteosarcoma stem cells (OSCs) are a potential cause of tumor metastasis, relapse, and chemotherapy resistance. Therefore, it is necessary to develop novel therapeutic drugs, which not only kill osteosarcoma cells but also target OSCs. This study aims to explore the anti‐osteosarcoma effects of Bruceine D (BD), a natural compound derived from Brucea javanica, and investigate its underlying mechanisms. Results demonstrated that BD could significantly inhibit cell proliferation and migration, induce cell cycle arrest, and promote apoptosis in osteosarcoma cells. Besides, BD could also suppress the sphere‐forming and self‐renewal ability of OSCs. Mechanistically, the inhibitory role of BD on osteosarcoma cell growth and migration including OSC stemness was partially executed through the inhibition of STAT3 signaling pathway. More importantly, BD showed significant anti‐osteosarcoma activity without obvious side effects in vivo. Collectively, the results of this study demonstrated that BD exerts a strong inhibitory effect on tumor growth and stem cell like traits of osteosarcoma which may be partially due to STAT3 inhibition, suggesting that BD maybe a promising therapeutic candidate against osteosarcoma.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Sphere-forming Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Bruceine D inhibits tumor growth and stem cell‐like traits of osteosarcoma through inhibition of STAT3 signaling pathway

Wang

Zhong

et al. 2019

Cancer Medicine

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Advances in the research of osteosarcoma stem cells and its related genes

Zhang

Wei

Weng

et al. 2022

Cell Biology International

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Osteosarcoma is a malignant tumor that often occurs in adolescents. There is an urgent need for new treatment options for osteosarcoma due to its poor prognosis after metastasis. Cancer stem cell (CSC) theory states that CSCs represent a small proportion of cancer cells. These CSC have self‐renewal ability and are closely associated with cancer growth and metastasis as well as chemotherapy resistance. Similarly, osteosarcoma stem cells (OSCs) play an important role in the growth, metastasis, and chemotherapy resistance of osteosarcoma cells. Targeting OSCs may represent a future treatment of osteosarcoma. Furthermore, some genes have been shown to regulate the growth, metastasis, and chemotherapy resistance of osteosarcoma cells by altering the stemness of OSCs. Targeting these genes may help in the treatment of osteosarcoma. This review mainly discusses recent advances in the research of OSCs and their related genes.

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The Heterogeneity of Osteosarcoma: The Role Played by Cancer Stem Cells

Schiavone

Garnier

Heymann

et al. 2019

Advances in Experimental Medicine and Biology

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Osteosarcoma is the most common bone sarcoma and is one of the cancer entities characterized by the highest level of heterogeneity in humans. This heterogeneity takes place not only at the macroscopic and microscopic levels, with heterogeneous micro-environmental components, but also at the genomic, transcriptomic and epigenetic levels. Recent investigations have revealed the existence in osteosarcoma of cancer cells with stemness properties. Cancer stem cells are characterized by their specific phenotype and low cycling capacity, and are linked to drug resistance, tumour growth and the metastatic process. In addition, cancer stem cells contribute to the enrichment of tumour heterogeneity. The present manuscript will describe the main characteristic features of cancer stem cells in osteosarcoma and will discuss their impact on maintaining tumour heterogeneity. Their clinical implications will also be briefly addressed.

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Melatonin inhibits osteosarcoma stem cells by suppressing SOX9-mediated signaling

Cited by 38 publications

References 51 publications

Bruceine D inhibits tumor growth and stem cell‐like traits of osteosarcoma through inhibition of STAT3 signaling pathway

Bruceine D inhibits tumor growth and stem cell‐like traits of osteosarcoma through inhibition of STAT3 signaling pathway

Advances in the research of osteosarcoma stem cells and its related genes

The Heterogeneity of Osteosarcoma: The Role Played by Cancer Stem Cells

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