Melatonin induction of HSP90 expression exerts cryoprotective effect on ovarian tissue

Cheng, Lu; Sun, Tie; Liu, Xiao Chao; Yu, Haiyang; Zhou, Shan; Tian, Li; Yang, Song; Liu, Bei Xing

doi:10.1016/j.cryobiol.2020.12.002

Cited by 7 publications

(4 citation statements)

References 16 publications

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“…Cryopreservation can damage mRNA and protein in oocytes, such as Hsp90 in mice (Cheng et al., 2021); SOD1, Bax and Bcl‐2 expression were all markedly downregulated in pig ovaries that had been cryopreserved (Dai et al., 2015). The recovery of ovarian function involves numerous signalling molecules, receptors and pathways (Celik et al., 2020; Lee, Cho, et al., 2021).…”

Section: Discussionmentioning

confidence: 99%

Melatonin activates Nrf2/HO‐1 signalling pathway to antagonizes oxidative stress‐induced injury via melatonin receptor 1 (MT1) in cryopreserved mice ovarian tissue

Wei,

Zhang,

et al. 2024

Reprod Domestic Animals

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Our previous research has shown that melatonin (MLT) can reduce cryopreserved ovarian damage in mice. Yet, the molecular mechanism of MLT protection is still unclear. Some studies have shown that melatonin receptor 1 (MT1) is very important for animal reproductive system. To evaluate whether MLT exerts its protective effect on cryopreserved mice ovarian tissue via MT1, we added antagonist of MT1/MT2 (Luzindor) or antagonist of MT2 (4P‐PDOT) to the freezing solution, followed by cryopreservation and thawing of ovarian tissue. The levels of total superoxide dismutase (T‐SOD), catalase (CAT), nitric oxide (NO) and malondialdehyde (MDA) were detected. Besides, by using RT‐PCR and Western blotting, the expression of Bcl‐2, Bax and Nrf2/HO‐1 signalling pathway‐related proteins was detected. These findings demonstrated that compared with the melatonin group, the addition of Luzindor increased apoptosis, NO and MDA activities, decreased CAT and T‐SOD activities and inhibited Nrf2/HO‐1 signalling pathway. In conclusion, melatonin can play a protective role in cryopreserved ovarian tissue of mice through MT1 receptor.

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Section: Discussionmentioning

confidence: 99%

Melatonin activates Nrf2/HO‐1 signalling pathway to antagonizes oxidative stress‐induced injury via melatonin receptor 1 (MT1) in cryopreserved mice ovarian tissue

Wei,

Zhang,

et al. 2024

Reprod Domestic Animals

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“…After measurement of the osmotic sensitivity of stromal cells, both suspended and adherent bovine OSCs were exposed to selected hypoosmotic (25 mOsm/kg and 150 mOsm/kg) and hyperosmotic (500 mOsm/kg and 1000 mOsm/kg) solutions containing concentrations of melatonin (MLT; 0 mM (control), 0.001 mM, 0.01 mM, 0.1 mM, 1 mM and 10 mM) (Sigma Aldrich, Burlington, MA, USA) and incubated for 15 min at 37 °C and 5% CO 2 , followed by a 5 min equilibration with isotonic culture media in the incubator. These concentrations of melatonin were selected based on previous studies [ 28 , 78 ]. To determine the optimal dose of melatonin, the cell viability and ROS production levels were evaluated in all groups.…”

Section: Methodsmentioning

confidence: 99%

Exogenous Melatonin Ameliorates the Negative Effect of Osmotic Stress in Human and Bovine Ovarian Stromal Cells

2022

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Ovarian tissue cryopreservation transplantation (OTCT) is the most flexible option to preserve fertility in women and children with cancer. However, OTCT is associated with follicle loss and an accompanying short lifespan of the grafts. Cryopreservation-induced damage could be due to cryoprotective agent (CPA) toxicity and osmotic shock. Therefore, one way to avoid this damage is to maintain the cell volume within osmotic tolerance limits (OTLs). Here, we aimed to determine, for the first time, the OTLs of ovarian stromal cells (OSCs) and their relationship with reactive oxygen species (ROS) and mitochondrial respiratory chain activity (MRCA) of OSCs. We evaluated the effect of an optimal dose of melatonin on OTLs, viability, MRCA, ROS and total antioxidant capacity (TAC) of both human and bovine OSCs in plated and suspended cells. The OTLs of OSCs were between 200 and 375 mOsm/kg in bovine and between 150 and 500 mOsm/kg in human. Melatonin expands OTLs of OSCs. Furthermore, melatonin significantly reduced ROS and improved TAC, MRCA and viability. Due to the narrow osmotic window of OSCs, it is important to optimize the current protocols of OTCT to maintain enough alive stromal cells, which are necessary for follicle development and graft longevity. The addition of melatonin is a promising strategy for improved cryopreservation media.

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“…Additionally, MT plays a central role in the reproductive system by upregulating LH receptor mRNA to suppress gonadotropin-releasing hormone (GnRH) and GnRH receptor expression as well [56]. As for the resource, MT in the ovary can be derived from sys-temic blood circulation or synthesized by granulosa cells, including the cumulus granulosa cells and oocytes [57][58][59][60]. MT1 and melatonin receptor type 2 (MT2) mRNAs can be detected in human granulosa cells and luteal cells [52].…”

Section: There Is a Strong Connection Between Melatonin (Mt) And Poimentioning

confidence: 99%

Therapeutic Effect of Melatonin in Premature Ovarian Insufficiency: Hippo Pathway Is Involved

Sun

Yang

et al. 2022

Oxidative Medicine and Cellular Longevity

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Objective. Premature ovarian insufficiency (POI) is a female reproductive disorder of unknown etiology with no definite pathogenesis. Melatonin (MT) is an endogenous hormone synthesized mainly by pineal cells and has strong endogenous effects in regulating ovarian function. To systematically explore the pharmacological mechanism of MT on POI therapy, a literature review approach was conducted at the signaling pathways level. Methods. Relevant literatures were searched and downloaded from databases, including PubMed and China National Knowledge Infrastructure, using the keywords “premature ovarian insufficiency,” “Hippo signaling pathways,” and “melatonin.” The search criteria were from 2010 to 2022. Text mining was also performed. Results. MT is involved in the regulation of Hippo signaling pathway in a variety of modes and has been correlated with ovarian function. Conclusions. The purpose of this review is to summarize the research progress of Hippo signaling pathways and significance of MT in POI, the potential crosstalk between MT and Hippo signaling pathways, and the prospective therapy.

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Melatonin induction of HSP90 expression exerts cryoprotective effect on ovarian tissue

Cited by 7 publications

References 16 publications

Melatonin activates Nrf2/HO‐1 signalling pathway to antagonizes oxidative stress‐induced injury via melatonin receptor 1 (MT1) in cryopreserved mice ovarian tissue

Melatonin activates Nrf2/HO‐1 signalling pathway to antagonizes oxidative stress‐induced injury via melatonin receptor 1 (MT1) in cryopreserved mice ovarian tissue

Exogenous Melatonin Ameliorates the Negative Effect of Osmotic Stress in Human and Bovine Ovarian Stromal Cells

Therapeutic Effect of Melatonin in Premature Ovarian Insufficiency: Hippo Pathway Is Involved

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