Melatonin in Sperm Biology: Breaking Paradigms

Cebrián‐Pérez, J. A.; Casao, A.; González-Arto, Marta; Hamilton, Thais Rose dos Santos; Pérez‐Pé, Rosaura; Muiño‐Blanco, T.

doi:10.1111/rda.12378

Cited by 40 publications

(28 citation statements)

References 182 publications

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“…either at 0 h or at 4 h), five different treatments were assayed: a positive control (C+), which consisted of spermatozoa incubated in CM containing bicarbonate and bovine serum albumin (BSA); three treatments with different concentrations of melatonin (0.5, 1, 5 lM) in CM; and a negative control (CÀ), which consisted of spermatozoa incubated in non-capacitating medium without bicarbonate or BSA (NCM). As aforementioned, the tested concentrations of melatonin were within the physiological range of the genital tract, as described in previous works (Luboshitzky et al, 2002;Casao et al, 2009Casao et al, , 2010Ashrafi et al, 2013;Cebri an-P erez et al, 2014;P erez-Patiño et al, 2016).…”

Section: In Vitro Capacitation and Progesterone-induced Acrosome Exocmentioning

confidence: 83%

“…The first one aimed at testing how melatonin affected the achievement of IVC (i.e. As aforementioned, the tested concentrations of melatonin were within the physiological range of the genital tract, as described in previous works (Luboshitzky et al, 2002;Casao et al, 2009Casao et al, , 2010Ashrafi et al, 2013;Cebri an-P erez et al, 2014;P erez-Patiño et al, 2016). The second part sought to determine the impact of melatonin upon triggering IVAE in fully in vitro capacitated boar spermatozoa (i.e.…”

Section: Seminal Samplesmentioning

confidence: 99%

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Melatonin affects the motility and adhesiveness of in vitro capacitated boar spermatozoa via a mechanism that does not depend on intracellular ROS levels

et al. 2018

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This work sought to address the effects of melatonin during in vitro capacitation (IVC) and progesterone-induced acrosome exocytosis (IVAE) in boar spermatozoa. With this purpose, two different experiments were set. In the first one, IVC and IVAE were induced in the absence or presence of melatonin, which was added either at the start of IVC or upon triggering the IVAE with progesterone. Different parameters were evaluated, including intracellular levels of peroxides and superoxides, free cysteine radicals and distribution of specific lectins. While melatonin neither affected most capacitation-associated parameters nor IVAE, it dramatically decreased sperm motility, with a maximal effect at 5 μm. This effect was accompanied by a significant increase in the percentage of agglutinated spermatozoa, which was independent from noticeable changes in the distribution of lectins. Levels of free cysteine radicals were significantly lower in melatonin treatments than in the control after 4 h of incubation in capacitating medium. The second experiment evaluated the effects of melatonin on in vitro fertilising ability of boar spermatozoa. Spermatozoa previously subjected to IVC in the presence of 1 μm melatonin and used for in vitro fertilisation exhibited less ability to bind the zona pellucida (ZP) and higher percentages of monospermy. In conclusion, melatonin affects sperm motility and the stability of nucleoprotein structure and also modulates the ability of in vitro capacitated boar spermatozoa to bind the oocyte ZP. However, such effects do not seem to be related to either its antioxidant properties or changes in the sperm glycocalix.

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Section: In Vitro Capacitation and Progesterone-induced Acrosome Exocmentioning

confidence: 83%

Section: Seminal Samplesmentioning

confidence: 99%

Melatonin affects the motility and adhesiveness of in vitro capacitated boar spermatozoa via a mechanism that does not depend on intracellular ROS levels

et al. 2018

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“…Melatonin (MLT) is a key regulator of the circadian wake–sleep cycle and reproduction in animals characterized by photoperiod (Chemineau et al, ); it has also a relevant role in sperm biology (Cebrián‐Pérez et al, ). In fact, MLT contrasts a variety of ROS species due to its well established antioxidant activity (Jang et al, ).…”

Section: Introductionmentioning

confidence: 99%

Effect of chelating and antioxidant agents on morphology and DNA methylation in freeze‐drying rabbit (Oryctolagus cuniculus) spermatozoa

Mercati

Domingo

Pasquariello

et al. 2019

Reprod Domestic Animals

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Freeze-drying (FD) has been exhaustively tried in several mammalian species as an alternative technique to sperm cryopreservation, but few studies have been done in rabbits (Oryctolagus cuniculus). The main objective of this study was to compare the protective effect of various antioxidants added to EDTA medium on structural and functional components of FD rabbit spermatozoa and on their status of global DNA methylation. FD media used were composed of basic FD medium (10 mM Tris-HCl buffer and 50 mM NaCl) supplemented with either 50 mM EDTA alone (EDTA) or added with 105 µM of rosmarinic acid (RA, EDTA-RA) or 10 µM of melatonin (MLT, EDTA-MLT). The effect of each medium on the preservation of FD spermatozoon structure was evaluated with light and scanning electron microscopy (SEM). Global DNA methylation was quantified in all FD sperm samples as well as in fresh spermatozoa. Morphologically, fracture points were evidenced in the neck, mid and principal piece of the spermatozoon tail. No differences in spermatozoon fracture pointswere evidenced among FD treatments: intact spermatozoa were the largest (p < .01) category, whereas the most frequent (p < .01) injury was the neck fracture, resulting in tailless heads. At SEM, the head of spermatozoa showed a well-conserved shape and intact membrane in all treatments. DNA methylation status was the same in all FD treatments. In conclusion, supplementation of EDTA, EDTA-RA and EDTA-MLT during FD preserved rabbit sperm morphological integrity and methylation status as well. Therefore, the difficulty of getting viable offspring using FD semen is likely unrelated to the impact of the lyophilization process on DNA methylation and morphology of lyophilized spermatozoa.

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“…On the other hand, melatonin is a key hormone in ovine reproduction. The melatonin main function is the regulation of seasonal sheep reproduction through the hypothalamus‐pituitary‐gonadal axis (Malpaux et al, ), although recent studies also suggest a direct action on sheep gametes and embryos, increasing their survival and quality (Abecia, Forcada, Casao, & Palacin, ; Cebrian‐Perez et al, ). Furthermore, we have detected the presence of melatonin‐synthesizing enzymes (Gonzalez‐Arto et al, ) and the melatonin receptors MT1 and MT2 in the testis and other organs of the ram reproductive tract (González‐Arto et al, ), as well as MT1 and MT2 receptors in ram spermatozoa (Casao et al, ).…”

Section: Introductionmentioning

confidence: 99%

Presence of melatonin‐catabolizing non‐specific enzymes myeloperoxidase and indoleamine 2,3‐dioxygenase in the ram reproductive tract

Martínez‐Marcos

Carvajal‐Serna

Lázaro‐Gaspar

et al. 2019

Reprod Domestic Animals

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The melatonin catabolism is very complex and not completely understood. Melatonin can be metabolized by free radical interaction, but also pseudo‐enzymatically or by enzymatic pathways. We have previously detected the existence of melatonin‐synthesizing enzymes and melatonin receptors MT1 and MT2 in the ram reproductive tract; thus, in order to start to elucidate melatonin catabolism in these organs, we have investigated the presence of the melatonin‐catabolizing enzymes indoleamine 2,3‐dioxygenase (IDO, both IDO1 and IDO2 isoforms) and myeloperoxidase (MPO) in testis, epididymis and accessory glands. Gene expression analyses by real‐time PCR showed the presence of MPO, IDO1 and IDO2 in all the organs of the ram reproductive tract and revealed that MPO is the main melatonin‐catabolizing enzyme, which is mainly expressed in the testis and the bulbourethral glands (p < .05). These results were further corroborated by immunohistochemical staining, and by Western blot. Likewise, MPO was also evidenced in epididymal and ejaculated spermatozoa by indirect immunofluorescence and Western blot. In conclusion, melatonin‐catabolizing enzymes MPO, IDO1 and IDO2 are expressed in the ram reproductive tract, and MPO is the most expressed one, mainly in the testis and the bulbourethral glands. The presented results warrant further studies on the function of these enzymes and their melatonin‐metabolizing activity.

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Melatonin in Sperm Biology: Breaking Paradigms

Cited by 40 publications

References 182 publications

Melatonin affects the motility and adhesiveness of in vitro capacitated boar spermatozoa via a mechanism that does not depend on intracellular ROS levels

Melatonin affects the motility and adhesiveness of in vitro capacitated boar spermatozoa via a mechanism that does not depend on intracellular ROS levels

Effect of chelating and antioxidant agents on morphology and DNA methylation in freeze‐drying rabbit (Oryctolagus cuniculus) spermatozoa

Presence of melatonin‐catabolizing non‐specific enzymes myeloperoxidase and indoleamine 2,3‐dioxygenase in the ram reproductive tract

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