Melatonin has dose-dependent effects on folliculogenesis, oocyte maturation capacity and steroidogenesis

Adriaens, I.; Jacquet, P.; Cortvrindt, R.; Janssen, Katharina; Smitz, Johan

doi:10.1016/j.tox.2006.09.018

Cited by 132 publications

(107 citation statements)

References 55 publications

(78 reference statements)

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“…In these studies, optimal blastocyst rates were achieved when physiological melatonin rates (Cos and Sanchez-Barceló, 2000), which varied from 10 -9 M to 10 -6 M, were added to culture media. In the present study, a concentration of melatonin close to physiological levels (10 -9 M) was tested because elevated concentrations of melatonin negatively affect follicle survival in culture (Adriaens et al, 2006).…”

Section: Resultsmentioning

confidence: 99%

“…Melatonin acts as a potent scavenger of free radicals (Tan et al, 2007) and a direct gonadal response to melatonin has been also reported, including stimulation of progesterone (Adriaens et al, 2006) and estradiol synthesis (Sirotkin and Schaeffer, 1997), and improvement of oocyte quality and maturation (Chattorag et al, 2005;Kang et al, 2009). Although there are some studies describing the beneficial effect of melatonin on in vitro embryo development in different species (Ishizuka et al, 2000;Papis et al, 2007;Rodriguez-Osorio et al, 2007), most of them report the addition of melatonin in embryo culture medium and in those which study the effect of melatonin during in vitro maturation, the maturation media were also supplemented with gonadotropins Kang et al, 2009;Manjunatha et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Melatonin in maturation media fails to improve oocyte maturation, embryo development rates and DNA damage of bovine embryos

Takada

Martins

Mingoti

et al. 2010

Sci. agric. (Piracicaba, Braz.)

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Melatonin (MEL) acts as a powerful scavenger of free radicals and direct gonadal responses to melatonin have been reported in the literature. Few studies, however, have evaluated the effect of MEL during in vitro maturation (IVM) on bovine embryos. This study tested the addition of MEL to maturation medium (MM) with no gonadotropins on nuclear maturation and embryo development rates and the incidence of DNA damage in resulting embryos. Cumulus-oocyte complexes were aspirated from abattoir ovaries and cultured in MM (TCM-199 medium supplemented with 10% fetal calf serum -FCS) at 39°C and 5% CO 2 in air. After 24 hours of culture in MM with 0.5 μg mL -1 FSH and 5.0 μg mL -1 LH; 10 -9 M MEL) or 10 -9 M MEL, 0.5 μg mL -1 FSH and 5.0 μg mL -1 LH, the oocytes were stained with Hoechst 33342 to evaluate nuclear maturation rate. After in vitro fertilization and embryo culture, development rates were evaluated and the blastocysts were assessed for DNA damage by Comet assay. There was no effect of melatonin added to the MM, alone or in combination with gonadotropins, on nuclear maturation, cleavage and blastocyst rates. These rates ranged between 88% to 90%, 85% to 88% and 42% to 46%, respectively. The extent of DNA damage in embryos was also not affected by MEL supplementation during IVM. The addition of 10 -9 M MEL to the MM failed to improve nuclear maturation and embryo development rates and the incidence of DNA damage in resulting embryos, but was able to properly substitute for gonadotropins during IVM. Key words: comet assay, embryos, gonadotropins, in vitro fertilization, melatonin Melatonina no meio de maturação não melhorou as taxas de maturação dos ovócitos, de desenvolvimento embrionário e a fragmentação do DNA dos embriões bovinos RESUMO: Melatonin (MEL) atua como um potente redutor de radicais livres. Efeito direto da MEL na função gonadal também foi observado. Existem poucos estudos relacionados ao efeito da MEL durante a maturação no desenvolvimento embrionário in vitro. Avaliou-se a adição de MEL no meio de maturação (sem gonadotrofinas) nas taxas de maturação nuclear e de desenvolvimento embrionário e na incidência de fragmentação do DNA nos embriões produzidos in vitro. Complexos cumulus-ovócitos foram aspirados de ovários provenientes de matadouros e cultivados em meio de maturação (Meio TCM-199 suplementado com 10% de soro fetal bovino) a 39°C e 5% CO 2 em ar. Após 24 horas de cultivo em meio de maturação com 0,5 μg mL -1 de FSH e 5,0 μg mL -1 de LH; 10 -9 M de MEL ou 10 -9 M de MEL, 0,5 μg mL -1 de FSH e 5,0 μg mL -1 de LH, os ovócitos foram corados com Hoechst 33342 para avaliação da taxa de maturação nuclear. A fragmentação do DNA dos blastocistos foi avaliada pela técnica do Cometa. Não houve efeito da MEL adicionada ao meio de maturação, com ou sem gonadotrofinas, nas taxas de maturação nuclear, clivagem e blastocistos. Os percentuais variaram entre 88% a 90%, 85% a 88% e 42% a 46%, respectivamente. O grau de fragmentação do DNA também não foi afetado pela adição de MEL ao meio de matur...

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Melatonin in maturation media fails to improve oocyte maturation, embryo development rates and DNA damage of bovine embryos

Takada

Martins

Mingoti

et al. 2010

Sci. agric. (Piracicaba, Braz.)

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“…A balance between the ROS production and the antioxidant levels is important for oocyte maturation and fertilization [21]. Melatonin, being one of the free radical scavengers and antioxidant agents, has been found to have a positive effect on the oocyte quality of IVF-ET patients [23,24,36,37]. Hemadi et al concluded that melatonin has a promoting effect on the quality of the cumulus-oocyte complex [23].…”

Section: Discussionmentioning

confidence: 99%

Melatonin improves the oocyte and the embryo in IVF patients with sleep disturbances, but does not improve the sleeping problems

Eryılmaz

Devran

Sarıkaya

et al. 2011

J Assist Reprod Genet

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Purpose We aimed to analyse the in vitro fertilizationembryo transfer (IVF-ET) outcomes of the patients with sleep disturbances who were administered melatonin. Methods A total of 60 patients with sleep disturbances were divided into two groups. The study group (group A, n=30) had underwent the IVF-ET with melatonin administration and the control group (group B, n=30) without melatonin. Sleeping status after melatonin administration and the IVF outcomes were compared between the two groups. Results Sleeping status change was not significant (p>0.05). The mean number of the retrieved oocytes, the mean MII oocyte counts, the G1 embryo ratio were significantly higher in the melatonin administered group (group A) than that the non-administered group (group B); p=0.0001; p=0.0001; p<0.05 respectively. Conclusion IVF patients with sleep disorders may benefit from melatonin administration in improving the oocyte and the embryo quality, but the sleeping problem itself may not be fixed.

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“…More recent studies had demonstrated that beside its multiple actions on different physiological processes, melatonin as well as its metabolites are indirect antioxidants and powerful direct scavengers that protected the cells from the free radicals brought about by their metabolism (Ahn and Bae, 2004b;2004a;Adriaens et al, 2006;Kang et al, 2009). Melatonin exerts its effects via two specific receptors termed as MT 1 and MT 2 (Reppert et al, 1994;.…”

Section: Introductionmentioning

confidence: 99%

Evidences for the Role of Melatonin as a Protective Additive During Buffalo Semen Freezing

Raey¹,

Badr²,

Rawash³

et al. 2014

American Journal of Animal and Veterinary Sciences

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Till now, the exact role of melatonin in male infertility hasn't been fully discovered. Moreover, the intracellular signaling pathways activated by melatonin in buffalo spermatozoa especially on the level of cryopreservation process haven't been reported. The current study aimed simply to clarify the precise role of this hormone during buffalo semen cryopreservation. Semen samples were obtained randomly from 6 fertile buffalo bulls (aged 3 to 5 years). Weekly, two consecutive ejaculates were collected from each bull for successive six weeks duration using an artificial vagina. The ejaculates were pooled to eliminate variability between the evaluated samples. After that, semen samples were subjected to extension with Tris-based extender supplemented with different concentrations of melatonin (0.1, 0.25, 0.5, 0.75 and 1 mM) Vs. Trisbased extender only (control). Then they were processed to cryopreservation and thawing to assess the different semen characteristics. It had been found that 0.1 mM of melatonin significantly (p<0.05) improved post thawing motility, viability index, acrosomal integrity, total antioxidant capacity, superoxide dismutase and glutathione reductase activity. On the other hand, it significantly (p<0.05) decreased the rate of lipid peroxidation, aspartate amino transferase, alanine aminotransferase, alkaline phosphatase and DNA fragmentation. All of these previously enhanced semen characteristics were reflected positively on its in vitro fertilizing capacity, as well as the percent of harvested embryos. In conclusion melatonin supplementation to the extension media of buffalo semen during processing significantly enhanced its characteristics. Moreover, the preceding results focusing more light on the potential roles of melatonin in regulating male reproduction.

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Melatonin has dose-dependent effects on folliculogenesis, oocyte maturation capacity and steroidogenesis

Cited by 132 publications

References 55 publications

Melatonin in maturation media fails to improve oocyte maturation, embryo development rates and DNA damage of bovine embryos

Melatonin in maturation media fails to improve oocyte maturation, embryo development rates and DNA damage of bovine embryos

Melatonin improves the oocyte and the embryo in IVF patients with sleep disturbances, but does not improve the sleeping problems

Evidences for the Role of Melatonin as a Protective Additive During Buffalo Semen Freezing

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