Melatonin enhances the in vitro and in vivo repair of severed rat sciatic axons

Stavisky, Ronda C.; Britt, Joshua M.; Zuzek, Aleksej; Truong, Elizabeth; Bittner, George D.

doi:10.1016/j.neulet.2004.11.033

Cited by 36 publications

(18 citation statements)

References 15 publications

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“…Most recently, PEG has been used to rapidly restore axonal morphological and physiological continuity of cut- or crushed- severed mammalian sciatic nerves in vitro , ex vivo and/or in vivo [3–6,11,12]. Recent studies have also demonstrated that PEG has a neuroprotective effect after acute spinal cord injury in the rat model [5,13].…”

Section: Introductionmentioning

confidence: 99%

Hydrophilic polymers enhance early functional outcomes after nerve autografting

Sexton

Pollins

Cardwell

et al. 2012

Journal of Surgical Research

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Background Approximately 12% of operations for traumatic neuropathy are for patients with segmental nerve loss and less than 50% of these injuries obtain meaningful functional recovery. Polyethylene glycol (PEG) therapy has been shown to improve functional outcomes after nerve severance and we hypothesized this therapy could also benefit nerve autografting. Methods A segmental rat sciatic nerve injury model was used, whereby a 0.5 cm defect was repaired with an autograft using microsurgery. Experimental animals were treated with solutions containing methylene blue (MB) and PEG; control animals did not receive PEG. Compound Actions Potentials (CAPs) were recorded before nerve transection, after solution therapy, and at 72 hours postoperatively. The animals underwent behavioral testing at 24 and 72 hours postoperatively. After sacrifice, nerves were fixed, sectioned, and immunostained to allow for quantitative morphometric analysis. Results The introduction of hydrophilic polymers greatly improved morphological and functional recovery of rat sciatic axons at 1–3 days following nerve autografting. PEG therapy restored CAPs in all animals and CAPs were still present 72 hours postoperatively. No CAPS were detectable in control animals. Footfall asymmetry scores and sciatic functional index scores were significantly improved for PEG therapy group at all time points (p <0.05 and p<0.001; p <0.001 and p <0.01). Sensory and motor axon counts were increased distally in nerves treated with PEG compared to control (p = 0.0189 and p = 0.0032). Conclusions PEG therapy improves early physiologic function, behavioral outcomes, and distal axonal density after nerve autografting.

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Section: Introductionmentioning

confidence: 99%

Hydrophilic polymers enhance early functional outcomes after nerve autografting

Sexton

Pollins

Cardwell

et al. 2012

Journal of Surgical Research

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“…They are used mainly in neurosurgery. 8,9 Albumin and glutaraldehyde-based products These mixed-origin products (natural albumin and synthetic glutaraldehyde) have excellent bonding ability in a mere 2-3 min. 10 These products are used in cardiac surgery.…”

Section: Polyethylene Glycol Polymersmentioning

confidence: 99%

Fibrin sealants in cardiac surgery: The last five years of their development and application

Novotný

Hlubocký²,

Mitáš³

et al. 2018

Adv Clin Exp Med

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This review article describes the use of fibrin glue or fibrin sealants and their development over the past 5 years, with a focus on cardiac surgery. The roles of various types of sealants that are available in hemostasis control are reviewed briefly, together with the various potential risks and side effects of their use. The results of experimental work reported during the last 5 years, clinical data from the same period and the safety aspects of fibrin-based glues and sealants are summarized, showing many advantages of their clinical application over the use of synthetic glues or sealants that may be stronger in some cases, but less safe. It can be concluded that the widespread use of fibrin sealants is fully justified, as it benefits the patient as well as the surgeon through the improved control of hemostasis without increasing any adverse effects or complications during surgical procedures.

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“…37 Stavisky et al found that a significantly higher percentage of crushed rat sciatic axons can be repaired in vitro and/or in vivo by plasmalemma fusion following exposure to MLT. 38 Erol et al observed that both MLT and octreotide gave benefits in an spinal cord injury (SCI) model, and MLT had the more pronounced beneficial effect. 39 Their biochemical and histopathological findings were correlated and significant.…”

Section: Free Radical Scavenging Effects Of Melatonin In Peripheral Nmentioning

confidence: 99%

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2017

J Brachial Plex Peripher Nerve Inj

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Melatonin enhances the in vitro and in vivo repair of severed rat sciatic axons

Cited by 36 publications

References 15 publications

Hydrophilic polymers enhance early functional outcomes after nerve autografting

Hydrophilic polymers enhance early functional outcomes after nerve autografting

Fibrin sealants in cardiac surgery: The last five years of their development and application

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